Figure 2.

LJ000328 inhibits hepcidin expression and improves the anemia of Tmprss6^−/− mice. Tmprss6^−/− male mice injected with LJ000328 (blue boxes) (n=3) were sacrificed 2 hours after injection and compared to mock-injected mice (grey boxes) (n=4). Mice were analyzed for liver (A) P-SMAD5 relative to total SMAD5 protein expression and (B) Hamp mRNA expression. Tmprss6^−/− male mice were injected with LJ000328 (pink boxes) (n=5) for seven weeks and compared with mock-injected mice (purple boxes) (n=5). Mice were analyzed for (C) liver Hamp mRNA expression, (D) serum iron content, (E) transferrin saturation, (F) hemoglobin, (G) hematocrit, and (H) mean corpuscular volume. For comparison, black boxes indicate levels measured in wild-type (WT) mice. Means ± standard error of the mean (SEM) are shown for hematological parameters, and box-and-whisker plots for Hamp ΔCt values. Mean comparisons were made by Student’s t-tests. **P<0.01.