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. 2020 Jul 12;25(14):3180. doi: 10.3390/molecules25143180

Figure 3.

Figure 3

Topo II inhibiting activity of BDHs in Topo II-mediated DNA relaxation assay. lane D: pBR322 DNA; lane T: pBR322 DNA + Topo II; lane E: pBR322 DNA + Topo II + etoposide (100 μM); other lanes: pBR322 DNA + Topo II + compounds. (A,B) BCHs at concentration of 50 μM; (C,D) BCHs at concentration of 20 μM; (E) BCHs at concentration of 10 μM; (F–H) semi-quantification of Topo II inhibiting activity of BCHs at 50, 20, 10 μM, respectively, with positive control (lane D: pBR322 DNA) as 100%, negative control (lane T: pBR322 DNA + Topo II) as 0% and comparative control (lane E: pBR322 DNA + Topo II + etoposide 100 μM).