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. 2020 Jun 29;12(7):1932. doi: 10.3390/nu12071932

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of the high-fat and high-cholesterol diet and metformin supplementation on (a) testicular histology, (b) thickness of the germinal epithelium (GE), (c) mean testicular biopsy score (MTBS) and (d) mean seminiferous tubule diameter (MSTD) in the male mice: Testicular sections were stained with hematoxylin and eosin. Data are expressed as means ± SD (n = 5 per group). * p < 0.05 as determined by one-way ANOVA followed by LSD post hoc test. NC, normal diet; HFC, high-fat and high-cholesterol diet; HFC + Met, HFC with metformin supplementation. Scale bar: 50 μm.