Figure 3.
Modulation of expression of sirtuins and transcription factors (PGC-1α and YY-1) by MIOX. Panels (A,B): immunoblotting data indicated a decreased expression of Sirt1, Sirt3 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and Ying-Yang1 (YY-1) following HFD administration in various strains of mice. The most notable reduction was seen in MIOX-TG mice prior to, and following, HFD treatment. The ob/ob mice also had reduced expression, and it was considerably restored in ob/MIOXKO mice. Panels (C–H): immunofluorescence microscopy revealed reduced expression of YY-1 in HK-2 cells (proximal tubular cell line) transfected with MIOX pcDNA, which was further decreased following treatment with palmitate-BSA (P-BSA). The exposure of MIOX-siRNA to P-BSA-treated HK-2 cells partially restored the expression of YY-1 (magnification: 200×). Panel (I): Western blot data indicated the changes in the expression of PGC-1α, which basically recapitulated the changes observed in YY-1 transcription factor in HK-2 cells after various treatments.