High expression of GPNMB indicates an unfavorable prognosis in glioma: Combination of data from the GEO and CGGA databases and validation in tissue microarray

Abstract

Glycoprotein non-metastatic melanoma protein B (GPNMB), a transmembrane glycoprotein, has been reported to be involved in tumor progression, but its prognostic value for glioma and the mechanistic effects on glioma progression have not been clearly explored. The present study aimed to investigate the prognostic role of GPNMB in glioma and the potential mechanisms of how GPNMB mediates glioma progression. Differentially expressed genes between the four highest and four lowest GPNMB expression samples in the GSE53733 dataset were first determined. Gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene set enrichment analysis results demonstrated that the significantly enriched pathways in samples with high GPNMB expression compared with those with low GPNMB expression were associated with hypoxia, angiogenesis, migration and invasion. Pearson correlation analysis was conducted to investigate the correlations between GPNMB expression and the markers of hypoxia, angiogenesis, migration and invasion in GSE53733, which were further validated using another mRNA microarray dataset from the Chinese Glioma Genome Atlas (CGGA). In addition, using the CGGA dataset, high GPNMB expression was demonstrated to be significantly associated with advanced WHO grade and short survival time in patients with glioma. Of note, based on the immunohistochemical staining of the tissue microarrays, Kaplan-Meier analysis with the Renyi test and a Cox proportional hazards model were used to validate the unfavorable prognostic role of high GPNMB expression in glioma. In conclusion, high GPNMB expression may be associated with high tumor grade and unfavorable prognosis in glioma. GPNMB expression was demonstrated to correlate with the markers of hypoxia, angiogenesis, migration and invasion, which may be potential mechanisms through which GPNMB mediates glioma progression.

Introduction

Glioma arises from glial or precursor cells of the central nervous system (CNS) and accounts for ~26% of all primary CNS tumors and >80% of malignant CNS tumors in the USA (1). Glioma has several histological subtypes, such as astrocytoma (including glioblastoma), oligodendroglioma and ependymoma among others (1,2). Despite continuing progress in treatments (such as surgery, chemotherapy, radiotherapy, targeted therapy and immunotherapy), the prognosis of glioma, particularly that of glioblastoma, remains unfavorable. For example, the 1- and 5-year survival rates of glioblastoma in the USA from 2000–2014 were estimated to be 41.4 and 5.4%, respectively (3). To date, although certain molecular alterations [e.g. isocitrate dehydrogenase 1 and 2 (IDH1/2) mutations] have been identified as prognostic markers for glioma (4,5), more prognostic biomarkers are required due to the complex genomic alterations (6) and biological heterogeneity (7) of glioma.

Glycoprotein non-metastatic melanoma protein B (GPNMB), a type I transmembrane glycoprotein, was originally cloned from metastatic human melanoma cell lines (8). GPNMB is not only expressed in normal tissues (e.g. skin, bone, urinary system and CNS tissues), but also abnormally expressed in pathological tissues such as glaucoma, colitis, liver injury and a variety of carcinoma tissues (9), such as breast (10), gastric (11) or pancreatic cancer (12). GPNMB is located on the cell membrane and intracellular organelles, such as melanosomes and lysosomes, and can also be secreted into the extracellular compartment (13–15).

GPNMB serves multiple roles in both normal and tumor tissues. In normal tissues, GPNMB modulates various physiological processes, such as melanosome maturation (14), intercellular adhesion between keratinocytes and melanocytes (16), osteoclast and osteoblast differentiation (15,17,18), and the regulation of immune responses (19,20). Regarding the role of GPNMB in cancer, a variety of studies have demonstrated pro-tumorigenic roles of GPNMB in breast (10,21), gastric (11), lung (22) and pancreatic cancer (12).

GPNMB has been demonstrated to be highly expressed in glioblastoma tissues (23) and to mediate glioma progression. Ono et al (24) have proposed that GPNMB prompts glioma progression by interacting with Na⁺/K⁺-ATPase α subunits. Using in vitro assays, Bao et al (25) demonstrated that GPNMB mediated the proliferation and migration of glioma cells and tube formation of endothelial cells. These studies attributed the mechanisms of GPNMB-mediated glioma progression to one single molecule; however, the molecular mechanisms underlying the GPNMB-induced glioma progression may involve numerous pathways or complicated networks and remain insufficiently characterized. To date, the prognostic role of GPNMB in glioma has been inadequately studied, although an early study from Kuan et al (23) suggested that GPNMB was associated with increasing survival risk for patients with glioblastoma. However, due to the limited sample size, specific ethnicity and other confounding factors in their study, the prognostic role of GPNMB in glioma requires further investigation.

Thus, the present study aimed to comprehensively elucidate the potential mechanisms of GPNMB-induced glioma progression and identify multiple pathways through which GPNMB may mediate glioma progression via systemic bioinformatics analysis.

Materials and methods

Publicly available datasets

The GSE53733 dataset (26), which comprises the data of Affymetrix gene chip analyses from 70 German patients with glioblastoma, was downloaded from the GEO database (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53733). Another publicly available dataset, which was originally used in a study by Yan et al (27), contained mRNA microarray data and clinical information from 220 Chinese patients with glioma and was downloaded from the CGGA database (http://www.cgga.org.cn/). The glioma patients in CGGA were classified into high and low GPNMB expression groups based on the median expression value of GPNMB.

Screening differentially expressed genes (DEGs)

To preliminarily explore the disparity in transcriptome profiles between patients with high and low GPNMB expression, the four highest (GSM1299519, GSM1299555, GSM1299571, GSM1299574) and four lowest GPNMB expression samples (GSM1299575, GSM1299580, GSM1299583, GSM1299584) in GSE53733 were compared. The raw data from the GSE53733 dataset were processed using the R Project version 3.5.3 (https://www.r-project.org/) (28). The DEGs between the top and bottom 4 GPNMB expression samples were identified using the limma package in R Project (29). The screening thresholds for DEGs were set at adjusted P-value=0.05 and log₂ fold-change=2.

Gene ontology (GO) term and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis

The upregulated DEGs in the four highest GPNMB expression samples compared with the four lowest samples were subjected to GO analysis using the Database for Annotation, Visualization and Integrated Discovery (https://david.ncifcrf.gov/). The enriched GO terms derived from the DEGs were categorized into three groups: Cell components (CC), molecular functions (MF) and biological processes (BP).

The enriched KEGG pathways of the DEGs were identified using the clusterProfiler package (30) and visualized using the pathview package (31) in R. The network diagram depicting complex interactions between significantly enriched KEGG pathways and DEGs was constructed using Cytoscape version 3.3.0 (https://cytoscape.org/).

Gene set enrichment analysis (GSEA)

GSEA between the top and bottom four samples was conducted using GSEA (version 2.2.3; http://software.broadinstitute.org/gsea/downloads.jsp). Enrichment scores of 0–1 and nominal P-values for enriched gene sets were calculated using this software.

Correlation analysis

Correlation analysis was performed between GPNMB and markers of angiogenesis, migration and invasion, including cluster of differentiation 31 (CD31), endoglin (ENG), C-X-C motif chemokine receptor 4 (CXCR4), transforming growth factor β1 (TGFB1), plasminogen activator, urokinase (PLAU), PLAU receptor (PLAUR) and matrix metalloproteinase 2 (MMP-2), MMP-7 and MMP-9. Pearson correlation analysis was used for parametric tests; Spearman correlation analysis was used for non-parametric tests. P-values for correlation analysis were determined using SPSS software (version 20.0.0; IBM Corp.).

Tissue microarray staining and scoring

The glioma tissue microarrays (G6042-3 and G6042-4) were purchased from Wuhan Servicebio Technology Co., Ltd. The tissue microarrays were subjected to immunohistochemical staining. Specifically, slides with tissue microarray were deparaffinized and rehydrated. Heat-induced antigen retrieval was conducted by immersing the slides into boiling Tris-EDTA buffer for 3 min. Slides were incubated with 3% hydrogen peroxide for 30 min to block endogenous peroxidase and then blocked with blocking buffer (cat. no. P0102; Beyotime Institute of Biotechnology) for 1 h at room temperature. Slides were incubated with an anti-GPNMB primary antibody (1:500; cat. no. 38313; Cell Signaling Technology, Inc.) overnight at 4°C. After washing the slides 3 times with Tris-Buffered Saline Tween-20 (TBST) for 5 min each time, the Real Envision Detection kit (cat. no. GK500710; GeneTech Biotechnology, Co., Ltd.) was used for signal visualization through the diaminobenzidine reaction. Nuclei were counterstained with hematoxylin. GPNMB immunoreactivity was scored by observing 3 random fields under a light microscope (Leica Microsystems, Inc.; magnification, ×200). GPNMB immunoreactivity was scored based on staining distribution and intensity as previously described (23). According to the percentage of immunopositive cells, the staining distribution was categorized as follows: 0 points, 0%; 1 point, 1–25%; 2 points, 26–50%; 3 points, 51–75%; and 4 points, 76–100%. The staining intensity was assessed and graded from 0 to 3 (0 points, negative; 1 point, weak staining; 2 points, moderate staining; and 3 points, strong staining). These two values were multiplied to achieve a maximum score of 12. Scores of 0–6 were considered low GPNMB expression, and scores of 7–12 were considered high GPNMB expression. The slides were evaluated by two independent observers. A total number of 74 Chinese patients with glioma were incorporated into the following analysis, after excluding those with incomplete follow-up information.

Cell culture and siRNA transfection

U-87MG (U87) glioblastoma cell line of unknown origin was obtained from the American Type Culture Collection (cat. no. HTB-14). U87 cells were cultured in Dulbecco's modified Eagle's medium (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.). Small interfering RNA (siRNA) targeting human GPNMB (5′-GGATAATACTGGCCTGTTT-3′) and a negative control siRNA (5′-GGCTCTAGAAAAGCCTATGC-3′) were purchased from Guangzhou RiboBio, Co., Ltd. A total of 5×10⁵ U87 cells were seeded in 6-well plates overnight and then transfected with 50 nM siRNA using Lipofectamine^® 2000 (cat. no. 11668-019; Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 6 h according to the manufacturer's instructions. Subsequent experiments were conducted 24 h post-transfection.

Western blotting

Cells were lysed for protein extraction with RIPA lysis buffer (cat. no. P0013B; Beyotime Institute of Biotechnology). Protein concentration was determined using an Enhanced BCA Protein Assay kit (cat. no. P0010; Beyotime Institute of Biotechnology). A total of 30 µg protein for each sample was loaded per lane for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using a 10% stacking gel to separate the proteins, which were subsequently transferred to 0.22-µm PVDF membranes. The PVDF membranes were blocked with 5% non-fat dry milk in Tris-buffered saline at room temperature for 1 h, washed three times with Tris-Buffered Saline Tween-20 and incubated with primary antibodies against GPNMB (dilution, 1:1,000; cat. no. 38313; Cell Signaling Technology, Inc.) or GAPDH (dilution, 1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.) overnight at 4°C. The membranes were then washed three times and incubated with horseradish peroxidase-conjugated secondary antibodies (1:5,000; cat. no. 111-035-003; Jackson ImmunoResearch, Inc.) at room temperature for 1 h. Finally, Immobilon^™ Western Chemiluminescent HRP Substrate (cat. no. P90719; EMD Millipore) was used to visualize signals.

Cell counting Kit-8 (CCK-8) assay

The CCK-8 assay (cat. no. CK04; Dojindo Molecular Technologies, Inc.) was performed in 96-well plates according to the manufacturer's instructions. A total of 3×10³ U87 cell were plated in each well of a 96-well plate. Prior to measuring, 10 µl of CCK-8 solution was added to each well and the plate was incubated for 2 h at 37°C. The absorbance at 450 nm was measured on days 1–3, and 4, respectively.

Cell migration and invasion assay

U87 migration and invasion assays were performed using 24-well Transwell plate inserts with 8-µm pores (cat. no. 3422; Corning, Inc.). For the cell invasion assay, the inserts were pre-coated with Matrigel (cat. no. 356234; Corning, Inc.) at 4°C and incubated at 37°C for 2 h. A total of 600 µl DMEM supplemented with 10% FBS was added into the lower chamber, and 200 µl serum-free medium containing 5×10⁵ U87 cells was added into the upper chamber. Following 24-h incubation, U87 cells in the upper chamber were removed with cotton swabs. Migratory or invasive U87 cells were fixed with 4% paraformaldehyde at room temperature for 1 h and stained with 0.1% crystal violet at room temperature for 15 min. The number of migratory or invasive U87 cells was determined by counting cells from five random fields under a light microscope (Leica Microsystems, Inc.) with ×200 magnification.

Statistical analysis

Data are presented as the mean ± standard error of the mean. In CCK-8 assay, 6 independent repeats for each group were performed and in the cell migration and invasion assays, 5 random fields were observed for cell counting. Statistical differences were calculated using SPSS Statistics (version 20; IBM, Inc.) and GraphPad Prism (version 6.02; GraphPad Software, Inc.). For parametric tests, unpaired Student's t-test was used for two-group comparisons, and one-way ANOVA followed by Tukey's post hoc test was conducted for multi-group comparisons. For non-parametric tests, Mann-Whitney U test was performed for two-group comparisons, and Kruskal-Wallis test followed by Dunn's multiple comparisons test was used. The Kaplan-Meier method followed by log-rank test was used for survival analysis. The Renyi test was performed to detect differences when survival curves crossed over with the survMisc package in R. The Cox proportional hazards model was used for univariate and multivariate analysis. P<0.05 was considered to indicate a statistically significant difference.

Results

Screening DEGs between samples with high and low GPNMB expression, and hierarchical clustering analysis

The four highest and four lowest GPNMB expression samples in the GSE53733 dataset were compared to screen for DEGs. Based on the aforementioned screening thresholds, 254 up- and 79 downregulated DEGs were identified and are presented in the volcano plot (Fig. 1A). Hierarchical clustering analysis was performed using the top 50 up- and downregulated DEGs between the four highest and four lowest GPNMB expression samples in GSE53733. The top 50 up- (Fig. 1B) and downregulated (Fig. 1C) DEGs are presented in the heat maps.

Figure 1.

Hierarchical clustering and GO analysis of the DEGs between the four highest and four lowest GPNMB expression samples. (A) Volcano plot of the DEGs (screening thresholds: Adj.P.Val=0.05; log₂ fold-change=2). Red spots represent the upregulated DEGs and green spots represent the downregulated DEGs in the high GPNMB expression group. (B and C) Heat maps of (B) the top 50 up- and (C) top 50 downregulated DEGs. (D-F) GO analysis revealed significantly enriched pathways of the upregulated DEGs in (D) BP, (E) CC and (F) MF. DEGs, differentially expressed genes; Adj.P.Val, adjusted P-value; GPNMB, glycoprotein non-metastatic melanoma protein B; GO, gene ontology; BP, biological process; CC, cellular component; MF, molecular function.

GO and KEGG analysis of DEGs

GO pathways in which the 79 downregulated DEGs were enriched were irrelevant to tumor biological behaviors and were therefore not studied (data not shown). The 254 upregulated DEGs were subjected to GO analysis. The significantly enriched GO terms in BP comprised ‘extracellular matrix organization’, ‘collagen catabolic process’, ‘collagen fibril organization’, ‘inflammatory response’ etc. (Fig. 1D). The significantly enriched GO terms in CC included ‘extracellular space’, ‘extracellular region’, extracellular matrix’, ‘extracellular exosome'etc. (Fig. 1E). The significantly enriched GO terms in MF contained ‘extracellular matrix structural constituent’, ‘integrin binding’, ‘heparin binding’, ‘collagen binding'etc. (Fig. 1F).

KEGG analysis using DEGs identified 38 significantly enriched pathways (Fig. 2A). To illustrate the interactions between the DEGs and the enriched KEGG pathways, a network diagram was constructed (Fig. 2B). The ‘ECM-receptor interaction’ and the ‘regulation of actin cytoskeleton’ are vital pathways mediating cell migration and invasion and are presented in detail in Fig. 2C and D.

Figure 2.

KEGG pathway enrichment analysis of DEGs. (A) Significantly enriched KEGG pathways of the DEGs. (B) Network diagram of the complex interactions among the DEGs and the significantly enriched KEGG pathways. (C) KEGG pathway of ‘ECM-receptor interaction’. (D) KEGG pathway of ‘actin cytoskeleton’. KEGG, Kyoto Encyclopedia of Genes and Genomes; DEGs, screening differentially expressed genes; ECM, extracellular matrix.

GSEA analysis

GSEA analysis between the four highest and four lowest GPNMB expression samples in GSE53733 identified the following significantly enriched pathways: ‘Hypoxia’ (Fig. 3A), ‘angiogenesis’ (Fig. 3B), ‘epithelial mesenchymal transition’ (Fig. 3C), ‘focal adhesion’ (Fig. 3D), ‘chemokine receptors bind chemokines’ (Fig. 3E), ‘chemokine receptor binding’ (Fig. 3F), ‘ECM component’ (Fig. 3G), ‘ECM binding’ (Fig. 3H) and ‘ECM receptor interaction’ (Fig. 3I).

Figure 3.

Gene Set Enrichment Analysis between the four highest and four lowest GPNMB expression samples in GSE53733. (A-I) Enriched pathways of (A) ‘hypoxia’, (B) ‘angiogenesis’, (C) ‘epithelial mesenchymal transition’, (D) ‘focal adhesion’, (E) ‘chemokine receptors bind chemokines’, (F) ‘chemokine receptor binding’, (G) ‘extracellular matrix component’, (H) ‘extracellular matrix binding’ and (I) ‘extracellular matrix receptor interaction’. GPNMB, glycoprotein non-metastatic melanoma protein B.

Correlation analysis between GPNMB and markers of angiogenesis, migration and invasion using the GSE53733 dataset and validation in the CGGA dataset

The GO and KEGG pathway analysis, as well as GSEA results revealed that DEGs between high and low GPNMB expression samples were generally enriched in pathways associated with angiogenesis, migration and invasion. Therefore, the present study subsequently investigated whether GPNMB expression correlated with the expression of the known markers of angiogenesis, migration and invasion. In the GSE53733 dataset, Pearson correlation analysis determined that GPNMB expression was positively correlated with the expression of CD31 (r=0.548; P<0.001), ENG (r=0.465; P<0.001; weak positive correlation), CXCR4 (r=0.584; P<0.001), TGFB1 (r=0.390; P<0.001; weak positive correlation), PLAU (r=0.557; P<0.001), PLAUR (r=0.657; P<0.001), MMP-2 (r=0.396; P<0.001; weak positive correlation), MMP-7 (r=0.341; P<0.001; weak positive correlation) and MMP-9 (r=0.496; P<0.001; weak positive correlation) (Fig. 4). Using a validation dataset from CGGA, Spearman correlation analysis revealed significant correlations between the expression of GPNMB and CD31 (rho=0.684; P<0.001), ENG (rho=0.502; P<0.001), CXCR4 (rho=0.661; P<0.001), TGFB1 (rho=0.334; P<0.001), PLAU (rho=0.631; P<0.001), PLAUR (rho=0.619; P<0.001), MMP-2 (rho=0.409; P<0.001), MMP-7 (rho=0.455; P<0.001) and MMP-9 (rho=0.518; P<0.001) (Fig. 5).

Figure 4.

Pearson correlation analysis between GPNMB and markers of angiogenesis, migration and invasion in GSE53733. (A-I) Correlations between GPNMB and (A) CD31, (B) ENG, (C) CXCR4, (D) TGFB1, (E) PLAU, (F) PLAUR, (G) MMP-2, (H) MMP-7 and (I) MMP-9. GPNMB, glycoprotein non-metastatic melanoma protein B; CD31, cluster of differentiation 31; ENG, endoglin; CXCR4, C-X-C motif chemokine receptor 4; TGFB1, transforming growth factor β1; PLAU, urokinase; PLAUR, PLAU receptor; MMP, matrix metalloproteinase.

Figure 5.

Spearman correlation analysis between GPNMB and markers of angiogenesis, migration and invasion in the mRNA microarray dataset in Chinese Glioma Genome Atlas. (A-I) Correlations between GPNMB and (A) CD31, (B) ENG, (C) CXCR4, (D) TGFB1, (E) PLAU, (F) PLAUR, (G) MMP-2, (H) MMP-7 and (I) MMP-9. GPNMB, glycoprotein non-metastatic melanoma protein B; CD31, cluster of differentiation 31; ENG, endoglin; CXCR4, C-X-C motif chemokine receptor 4; TGFB1, transforming growth factor β1; PLAU, urokinase; PLAUR, PLAU receptor; MMP, matrix metalloproteinase.

In addition, correlation analysis revealed that GPNMB expression was positively associated with CD163 in the GSE53733 dataset (r=0.671; P<0.001; Fig. S1A) and the microarray dataset in CGGA (rho=0.670; P<0.001; Fig. S1B).

In vitro experiments investigating the effects of GPNMB on glioma cell proliferation, migration and invasion

Fig. S2A demonstrates the knockdown of GPNBM in U87 cells transfected with siRNA. Subsequent experiments revealed that knockdown of GPNBM significantly inhibited the proliferation, migration and invasion of U87 cells (Fig. S2B-D).

Clinical and prognostic role of GPNMB expression in glioma

Using the mRNA microarray data and clinical information of the 220 Chinese patients with glioma from the CGGA, the clinical and prognostic role of GPNMB expression in glioma was investigated. Compared with non-tumor brain tissues, higher levels of GPNMB expression were observed in glioma tissues (Fig. 6A). The four gene expression-based molecular subtypes (proneural, neural, classical and mesenchymal) of glioblastoma were previously identified and characterized (32). The highest levels of GPNMB expression were identified in patients with the mesenchymal subtype (Fig. 6B). Regarding the association between histological subtypes and GPNMB expression, the highest levels of GPNMB expression were detected in patients with glioblastoma (Fig. 6C). GPNMB expression was higher in high-grade glioma (WHO III or IV) compared with that in low-grade glioma (WHO II) (Fig. 6D). Lower Karnofsky performance scores were observed in patients with high GPNMB expression compared with those with low expression (Fig. 6E). Kaplan-Meier analysis with the log-rank test demonstrated that high GPNMB expression was associated with a shorter survival time (P<0.001; Fig. 6F). Survival analysis in sub-populations stratified by age revealed that high GPNMB expression was significantly associated with a lower survival rate in patients aged 31–50 years (P<0.001; Fig. 6H) and 51–70 years (P=0.017; Fig. 6I), but not in patients aged 10–30 years (P=0.954; Fig. 6G).

Figure 6.

The association between GPNMB expression and clinicopathological characteristics of 220 patients with glioma from the Chinese Glioma Genome Atlas dataset explored using the mRNA microarray data and clinical information. (A) Compared with non-tumor brain tissues, higher levels of GPNMB expression were observed in glioma tissues. (B and C) The GPNMB expression levels were compared in the (B) four gene expression-based and (C) four histological subtypes. (D) The levels of GPNMB expression in different WHO grades were compared. (E) Lower KPS were identified in patients with high GPNMB expression. (F) Kaplan-Meier analysis with the log-rank test demonstrated that high GPNMB expression was associated with shorter survival time compared with that of patients with low GPNMB expression. (G and H) Survival differences were compared between patients with high and low GPNMB expression in sub-populations aged (G) 10–30, (H) 31–50 and (I) 51–70 years. *P<0.05, **P<0.01 and ***P<0.001. GPNMB, glycoprotein non-metastatic melanoma protein B; KPS, Karnofsky performance scores.

To validate the prognostic role of GPNMB in glioma, tissue microarrays were immunohistochemically stained with an anti-GPNMB antibody. Microarrays from 74 Chinese patients with glioma with complete follow-up information were analyzed. Representative images of GPNMB staining with different intensity scores are presented in Fig. 7A. No significant differences in GPNMB expression distribution were observed between female and male patients (χ²=0.173; P=0.678; Table I). The proportion of patients with high GPNMB expression was higher in patients aged >40 years compared with that in patients aged ≤40 years (χ²=5.316; P=0.021; Table I). The GPNMB expression level was significantly correlated with the WHO grade (rho=0.353; P=0.002; Table I), and the GPNMB expression scores were higher in high-compared with low-grade glioma (P=0.018; Fig. 7B). Survival analysis using Renyi test demonstrated that high GPNMB expression was significantly associated with a lower survival rate (P=0.002; Fig. 7C). In addition, multivariate analysis based on the Cox proportional hazards model revealed that GPNMB expression was an independent prognostic factor for glioma (P=0.009, Table II).

Figure 7.

Prognostic role of GPNMB in glioma confirmed in tissue microarrays. (A) Representative images of immunohistochemical staining against GPNMB with different intensity scores. (B) The immunohistochemical scores of GPNMB expression were higher in high-grade (WHO III and IV) compared with low-grade (WHO I and II) glioma. (C) The Renyi test demonstrated that high GPNMB expression was significantly associated with a lower survival rate compared with that in the low GPNMB expression group (P=0.002). GPNMB, glycoprotein non-metastatic melanoma protein B; WHO, World Health Organization.

Table I.

Association of GPNMB expression with clinical parameters in the 74 glioma patients.

	GPNMB expression
Variable	Low	High	Statistics value	P-value
Sex
Female	17	7	0.173 (χ2)	0.678
Male	33	17
Age
≤40	22	4	5.316 (χ2)	0.021a
>40	28	20
WHO gradeb
I	1	0	0.353 (Spearman rho)	0.002a
II	20	4
III	18	6
IV	11	14

^aP<0.05. GPNMB, glycoprotein non-metastatic melanoma protein B; WHO, World Health Organization.

^bPerry A and Wesseling P: Histologic classification of gliomas. Handbook of clinical neurology 134: 71–95, 2016.

Table II.

Univariate and multivariate analysis based on Cox proportional hazards model explored prognostic role of GPNMB.

		Multivariate analysis
Variable	Univariate analysis P-value	P-value	RR	95% CI
Sex (female vs. male)	0.281	0.181	0.637	0.330–1.232
Age (≤40 vs. >40)	0.702	0.409	1.340	0.669–2.686
WHO grade (I and II vs. III and IV)b	0.043a	0.114	0.562	0.275–1.148
GPNMB expression (low vs. high)	0.005a	0.009a	0.413	0.213–0.803

^aP<0.05. RR, risk ratio; CI, confidence interval; GPNMB, glycoprotein non-metastatic melanoma protein B; WHO, World Health Organization.

^bPerry A and Wesseling P: Histologic classification of gliomas. Handbook of clinical neurology 134: 71–95, 2016.

Discussion

To date, although a number of predictive molecular markers (e.g., 1p/19q co-deletion and IDH1/2 mutations) have been identified as prognostic markers for glioma (33–35), more are urgently required, partly due to the great complexity of the molecular traits of glioma (36). GPNMB, a transmembrane glycoprotein, has been demonstrated to be involved in tumor progression (11,12,21,22), whereas its mechanistic effects on glioma progression and its prognostic role have not been thoroughly investigated. Therefore, the aim of the present study was to elucidate the prognostic role of GPNMB in glioma through data mining of publicly accessible datasets through validation by immunohistochemical staining of a tissue microarray. Systematic bioinformatics analysis was conducted to explore the molecular disparities between samples with high and low GPNMB expression; the results identified potential mechanisms through which GPNMB may mediate glioma progression.

In the present study, the upregulated DEGs in samples with high GPNMB expression compared with samples with low GPNMB expression were subjected to GO enrichment analysis. The processes of cell migration and invasion have been established to be inextricably linked with and characterized by the extracellular matrix (ECM), cell adhesion, cell cytoskeleton and chemotaxis (37–41). In the present study, the most significantly enriched GO pathways participated in migration and invasion. In addition, KEGG analysis results consistently revealed that the DEGs were significantly enriched in pathways associated with cell migration and invasion, such as ‘ECM-receptor interaction’, ‘focal adhesion’, ‘regulation of actin cytoskeleton’, ‘chemokine signaling pathway’ and ‘cell adhesion molecules’. The subsequent GSEA demonstrated that glioma samples with high GPNMB expression were characterized by enriched pathways involved in migration and invasion. Correlation analysis was performed to explore the relationship between GPNMB and markers of migration and invasion. GPNMB expression was positively correlated with that of CXCR4, TGFB1, PLAU, PLAUR, MMP-2, MMP-7 and MMP-9, which are considered to be markers of tumor cell migration and invasion (42–51). Taken together, the comprehensive bioinformatics analysis, which included GO, KEGG, GSEA and correlation analysis, indicated the potential pathways through which GPNMB promotes glioma migration and invasion.

To the best of our knowledge, studies concerning the effects of GPNMB on glioma angiogenesis are rare. Using in vitro assays, Bao et al (25) demonstrated that GPNMB knockdown in glioma cells suppressed the tube formation of endothelial cells induced by conditioned medium from glioma cells. Considering that glioma angiogenesis is a highly complicated in vivo process (52), evidence from Bao et al (25) for the role of GPNMB in glioma angiogenesis may be reasonable but inadequate. In the present study, GO analysis revealed that the upregulated DEGs in samples with high GPNMB expression were enriched in ‘response to hypoxia’ and ‘positive regulation of angiogenesis’. Consistently, GSEA revealed that samples with high GPNMB expression were characterized by hypoxia and angiogenesis. In addition, GPNMB was positively correlated with CD31 and ENG, which are markers of angiogenesis (53,54). Taken together, based on systemic bioinformatics analysis, the results of the present study suggested that the effects of GPNMB on angiogenesis may be crucial mechanisms of glioma progression.

In addition to the effects of GPNMB on glioma angiogenesis, migration and invasion, several recent studies have suggested other potential mechanisms of GPNMB-mediated glioma progression which involve glioma-associated microglia/macrophage (GAM) polarization towards the M2 phenotype (55–57). GAM with the M2 phenotype has protumoral and immunosuppressive properties (58,59). Thus, the present study explored the correlation between GPNMB and CD163 (a known marker for GAM with the M2 phenotype) expression (60). In the present study, the correlation analysis revealed that GPNMB expression was positively associated with CD163 in the GSE53733 dataset and the microarray dataset in CGGA. In addition, GPNMB expression was significantly correlated with that of TGFB1, which is another known marker for GAM polarization towards the M2 phenotype (60). Although the correlation analysis indicated positive associations between GPNMB and markers of M2-phenotype GAM, the specific roles of GPNMB in GAM polarization require more thorough investigation and solid evidence.

To further validate the effects of GPNMB on glioma progression, in vitro experiments were performed to determine the role of GPNMB in glioma cell proliferation, migration and invasion. Fig. S2 demonstrates that knockdown of GPNBM significantly inhibited the proliferation, migration and invasion of U87 cells. These in vitro experiments consolidated the tumor-promoting role of GPNBM in glioma progression.

The prognostic role of GPNMB in glioma is not fully determined, although an early study from Kuan et al (23) suggested that GPNMB was associated with higher risk of death. Due to the limited sample size in survival analysis (only 39 cases), ethnicity, age, sex, lifestyle, other factors and the prognostic role of GPNMB in glioma requires further investigation. In the present study, it was revealed through data mining of the dataset from CGGA and subsequent validation with immunohistochemical staining of tissue microarray that high GPNMB expression, both at the mRNA and protein level, was associated with poor survival. Of note, multivariate analysis demonstrated that high GPNMB expression was an independent risk factor for patient survival. The difference in survival outcomes between patients with high and low GPNMB expression was not statistically significant in sub-populations aged <30 years, which may be due to the low percentage of patients with high GPNMB expression among young patients. In the present study, the population included in the survival analysis comprised Chinese patients, whereas the previous survival analysis was performed in American population (23), suggesting that the unfavorable prognostic effect of GPNMB on glioma may not be associated with ethnicity.

In conclusion, the results of the present study demonstrated that high GPNMB expression was associated with high malignancy and denoted unfavorable prognosis in patients with glioma. In addition, systematic bioinformatics analyses revealed that GPNMB expression was correlated with that of genes involved in hypoxia, angiogenesis, migration and invasion, which may be the potential mechanisms through which GPNMB mediates glioma progression.

Funding

This study was funded by the National Natural Science Foundation of China (grant nos. 81803048 to XF and 81602663 to WT) and the Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University (grant nos. ZC20170016 and ZC20180135 to XF and ZC23456049 to LZ).

Availability of data and materials

The datasets used and/or analyzed in the present study are available from the corresponding author upon reasonable request.

Authors' contributions

XF, WT and SC conceived and designed the present study. XF, LZ, SK, TL, LH and PZ performed the research, collected and analyzed the data, interpreted the results and wrote the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

The present study was approved by the Ethics Committee of Henan Provincial People's Hospital.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.