Skip to main content
NCBI home page
Experimental and Therapeutic Medicine logoLink to Experimental and Therapeutic Medicine
. 2020 Jun 19;20(3):2227–2235. doi: 10.3892/etm.2020.8918

Mechanism underlying the significant role of the miR-4262/SIRT1 axis in children with inflammatory bowel disease

Xiaozhi Deng 1, Lihong Shang 1, Min Du 1, Lan Yuan 1, Lijing Xiong 1, Xiaoli Xie 1,✉
PMCID: PMC7401569  PMID: 32765699

Abstract

Inflammatory bowel disease (IBD) is a term used to describe chronic and recurrent gastrointestinal disease. In total, >2 million individuals worldwide have been diagnosed with IBD, including ulcerative colitis (UC), Crohn's disease (CD) and indeterminate colitis. There is accumulating evidence to indicate that microRNAs (miRNAs or miRs) are involved in the development and progression of IBD. miR-4262, an underlying promoter in tumor diseases, has been reported to regulate inflammatory responses. However, the potential mechanisms underlying the role of miR-4262 in IBD remain unknown. The present study attempted to explore the role and mechanisms of miR-4262 in IBD. Firstly, reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of miR-4262 in 30 IBD colonic mucosa tissues, 30 normal tissues, 2% dextran sulfate sodium (DSS)-treated Caco-2 cells and normal cells. It was demonstrated that the expression levels of miR-4262 in IBD colonic mucosa tissues and 2% DSS-stimulated Caco-2 cells were markedly higher compared with those in the control groups. Target gene prediction databases and dual-luciferase reporter assays were then used, and sirtuin 1 (SIRT1) was identified as a target gene of miR-4262. Furthermore, the levels of SIRT1 in 2% DSS-stimulated Caco-2 cells and IBD colonic mucosa tissues were suppressed compared with the corresponding control groups. In addition, it was observed that miR-4262 negatively regulated SIRT1 expression in Caco-2 cells. Thereafter, Caco-2 cells were treated with inhibitor control, miR-4262 inhibitor, control-siRNA or SIRT1-siRNA for 48 h, followed by 2% DSS treatment for 4 days. The secretion of inflammatory factors was analyzed via ELISA and RT-qPCR. MTT assay, flow cytometry and western blot analysis were performed to assess cell viability, apoptosis and NF-κB signaling pathway-related protein levels, respectively. The results indicated that DSS enhanced the inflammatory response, suppressed cell viability and promoted cell apoptosis, and this was decreased following transfection with an miR-4262 inhibitor. In addition, 2% DSS upregulated p-p65 expression and enhanced the ratio of p-p65/p65, while the miR-4246 inhibitor exerted an opposite effect. All the effects of miR-4262 inhibitor on Caco-2 cells were eliminated following transfection with SIRT1-siRNA. It was thus concluded that miR-4262 may serve a role in the progression of IBD via targeting SIRT1, and miR-4262/SIRT1 may represent a potential target for the diagnosis and treatment of IBD.

Keywords: inflammatory bowel disease, microRNA, miR-4262, SIRT1

Introduction

Inflammatory bowel disease (IBD), a gastrointestinal tract disease, is regulated by the inflammatory response and is associated with symptoms such as gastrointestinal bleeding, colonic shortening, abdominal pain, diarrhea and colonic chronic inflammation (1,2). Ulcerative colitis (UC) and Crohn's disease (CD) are the two main types of IBD (3). Recently, the inflammatory response has been demonstrated to accelerate the pathogenesis of IBD (4). Although numerous efforts have been made to elucidate the mechanisms underlying IBD, the exact pathogenesis and mechanisms of IBD remain unclear. Therefore, anti-inflammatory drugs and the recognition of specific IBD agents may serve as novel therapeutic approaches for IBD.

MicroRNAs (miRNAs or miRs) are small non-coding RNA molecules that can regulate target gene levels or cell behaviors by degrading mRNAs or restricting translational levels (5). Multiple reports have confirmed that the abnormal expression of miRNAs is highly associated with various diseases, including IBD (6-8). Moreover, certain miRNAs exhibit anti-inflammatory effects and can be used as potential therapeutic targets. For instance, Huang et al (9) reported that the inhibition of miRNA-210 suppresses the pro-inflammatory response and reduces acute brain injury due to ischemic stroke in mice. In addition, Kumar et al (10) demonstrated that miRNA-26a modulates the inflammatory response induced by Toll-like receptor 4 stimulation in microglia. miR-4262, a recently discovered miRNA, has been identified as a biomarker and has been demonstrated to serve a promotive role in various diseases, including breast cancer (11), colon cancer (12) and cutaneous malignant melanoma (13). However, whether miR-4262 influences the development of IBD has not yet been reported, to the best of our knowledge.

Sirtuin 1 (SIRT1) serves a role in a number of biological functions, such as the inflammatory responses, cell apoptosis and signaling pathway regulations (14,15). However, whether SIRT1 serves a role in the progression of IBD and the underlying molecular mechanisms requires further elucidation. It has been indicated that dextran sodium sulfate (DSS) can be used to establish an intestinal in vitro barrier model due to its rapidity, simplicity and controllability (16,17).

The present study attempted to explore the role and mechanisms of miR-4262 in IBD. The expression of miR-4262 in IBD colonic mucosa tissues, normal tissues, DSS-treated Caco-2 cells and normal Caco-2 cells was determined. The role of miR-4262 in DSS-induced inflammation was investigated in the intestinal epithelial cell line, Caco-2, and the possible mechanisms of action of miR-4262 in IBD were analyzed.

Materials and methods

Clinical tissue samples

Between December 2016 and December 2018, colonic mucosa tissues were collected from 30 children with IBD (15 males; 15 females; age range: 7-11 years; mean age: 9.4 years) and 30 children without IBD (15 males; 15 females; age range: 6-12 years; mean age: 8.8 years) at Chengdu Women's and Children's Central Hospital (Chengdu, China). Patients with infectious colitis and colorectal cancer were excluded. Liquid nitrogen was employed to preserve the specimens at -80˚C until use. All specimens were obtained with written informed consent and the present study was approved by the Ethics Committee of Chengdu Women's and Children's Central Hospital.

Caco-2 cell culture and DSS treatment

The normal intestinal epithelial cell line Caco-2 was purchased from American Type Culture Collection. The cells were cultured in Dulbecco's modified Eagle medium (DMEM)/F12 (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal calf serum (FBS; HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin and were maintained at 37˚C with 5% CO2 in an incubator. Caco-2 cells were treated with 2% DSS at 37˚C for 4 days to construct the inflammatory model (18).

Reverse transcription-quantitative PCR (RT-qPCR)

SIRT1, inflammatory cytokines [interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α] and miR-4262 levels were measured via RT-qPCR. The isolation of RNA from colonic mucosa tissues and Caco-2 cells was performed using the RNA-isolation kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. cDNA was obtained using a PrimeScript RT kit (Takara Bio, Inc.) according to the manufacturer's protocol, followed by PCR amplification on an ABI Prism 7500 system (Applied Biosystems; Thermo Fisher Scientific, Inc.) with SYBR Premix Ex-Taq (Takara Bio, Inc.). miRNA and mRNA expression were normalized to U6 and GAPDH, respectively. The reaction conditions were as follows: Initial denaturation at 95˚C for 5 min; 30 cycles of 94˚C for 30 sec, 60˚C for 30 sec and 72˚C for 1 min; and a final extension at 72˚C for 5 min. Primers were produced by Sangon Biotech Co., Ltd. and the sequences were as follows: miR-4262 forward, 5'-TGCGGGACATTCAGA-3' and reverse, 5'-CCAGTGCAGGGTCCGAGGT-3'; SIRT1 forward, 5'-AATCCAGTCATTAAAGGTCTACAA-3' and reverse, 5'-TAGGACCATTACTGCCAGAGG-3'; TNF-α forward, 5'-GAACTGGCAGAAGAGGCACT-3' and reverse 5'-GGTCTGGGCCATAGAACTGA-3'; IL-1β forward, 5'-TGTGAAATGCCACCTTTTGA-3' and reverse, 5'-TGAGTGATACTGCCTGCCTG-3'; IL-6 forward, 5'-CCGGAGAGGAGACTTCACAG-3' and reverse, 5'-CAGAATTGCCATTGCACA-3'; U6 forward, 5'-GCTTCGGCAGCACATATACTAAAAT-3' and reverse, 5'-CGCTTCACGAATTTGCGTGTCAT-3'; and GAPDH forward, 5'-CTTTGGTATCGTGGAAGGACTC-3' and reverse, 5'-GTAGAGGCAGGGATGATGTTCT-3'. The relative results were analyzed using the 2-ΔΔCq method (19).

Cell transfection

The miR-4262 inhibitor and inhibitor control were synthesized by Shanghai Genepharma Co. Ltd. Caco-2 cells were cultured to 70-80% confluency and transiently transfected with 100 nM miR-4262 inhibitor (cat no. HSTUD1216; Sigma-Aldrich; Merck KGaA), 100 nM inhibitor control (cat no. HMC0002; Sigma-Aldrich; Merck KGaA), 0.2 µM control-siRNA (cat no. sc-36869; Santa Cruz Biotechnology, Inc.) or 0.2 µM SIRT1-siRNA (cat no. sc-40986; Santa Cruz Biotechnology, Inc.) using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's instructions. Following transfection, the cells were stimulated with 2% DSS at 37˚C for 4 days for further analysis.

Dual-luciferase reporter assay

TargetScan 7.2 (http://www.targetscan.org/vert_72/) was adopted to investigate the putative binding sites. The data suggested that SIRT1 was a potential target of miR-4262. Subsequently, the miR-4262 binding sites on SIRT1 3'-UTR, including wild-type or mutant, were inserted into the pGL-control vector (Promega Corporation) to construct a wild-type SIRT1 plasmid (SIRT1-WT) or SIRT1 mutation plasmid (SIRT1-MUT), respectively. The mimic control or miR-4262 mimic (Shanghai GenePharma Co., Ltd.) were then co-transfected with luciferase reporter vectors into Caco-2 cells using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's protocol. The luciferase activity was assessed using the Dual-Luciferase Reporter assay system (Promega Corporation) following the manufacturer's protocol. All luciferase activities were normalized to Renilla luciferase activity.

Enzyme-linked immunosorbent assay (ELISA)

ELISAs (all Beyotime Institute of Biotechnology) was adopted to measure the levels of TNF-α (cat. no. PT518), IL-6 (cat. no. PI330) and IL-1β (cat. no. PI305) in the cell supernatant following treatment. The optical density (OD) value of each well at 450 nm was detected, according to the manufacturer's instructions.

MTT assay

Cell viability was evaluated via MTT assay. The Caco-2 cells were cultured in 96-well plates at 37˚C, and the cells were then transfected with miR-4262 inhibitor, inhibitor control, control-siRNA and SIRT1-siRNA for 48 h and treated with 2% DSS (18) at 37˚C for a further 4 days. Following treatment, 10 µl MTT solution (Amresco LLC) was added to the cells, followed by incubation at 37˚C for 4 h, and DMSO (Sangon Biotech Co., Ltd.) was then used to dissolve the formazan crystals. Finally, the OD values at 490 nm were detected using a multifunctional plate reader (BD Biosciences).

Flow cytometric analysis

Caco-2 cell apoptosis was detected via flow cytometric analysis. In the present study, the cells were transfected with miR-4262 inhibitor, inhibitor control, control-siRNA and SIRT1-siRNA for 48 h and treated with 2% DSS for 4 days. Subsequently, the cells were trypsinized at room temperature for 1 min and stained with Annexin V-FITC/propidium iodide (PI; Beyotime Institute of Biotechnology), according to the manufacturer's protocol. The results were analyzed using a FACSCalibur flow cytometer (BD Biosciences) and FlowJo software (version 7.6.1; Tree Star, Inc.).

Western blot analysis

Total proteins from Caco-2 cells were extracted using RIPA buffer (Invitrogen; Thermo Fisher Scientific, Inc.) and quantified using a BCA Protein assay kit (Invitrogen; Thermo Fisher Scientific, Inc.). The denatured proteins were loaded on 10% SDS-PAGE, and transferred to PVDF membranes (Bio-Rad Laboratories, Inc.). After blocking with 5% skim milk in PBST at room temperature for 90 min, the membranes were cultured with primary antibodies against GAPDH (cat no. G9545), SIRT1 (cat no. S5447), phosphorylated (p)-p65 (cat no. SAB4504488) and p65 (cat no. SAB4502609; all from Sigma-Aldrich; Merck KGaA; 1:1,000) overnight at 4˚C. Horesradish peroxidase-conjugated oat anti-rabbit immunoglobulin G secondary antibody (cat no. ab7090; Abcam; 1:2,000) was incubated with the membranes at room temperature for 90 min. Finally, the results were quantified using Enhanced Chemiluminescence detection system reagents (EMD Millipore), according to the manufacturer's instructions.

Statistical analysis

GraphPad Prism 6 software (GraphPad Software, Inc.) was used for statistical analyses. All results are expressed as the mean ± standard deviation from three independent experiments. Group comparisons between multiple groups were performed by one-way ANOVA followed by Tukey's post hoc test. Unpaired Student's t-test was used to make comparisons between two groups. P<0.05 was considered to indicate a statistically significant difference.

Results

miR-4262 is upregulated in 2% DSS-stimulated Caco-2 cells and IBD colonic mucosa tissues

To analyze the role of miR-4262 in IBD, the expression of miR-4262 in 30 IBD colonic mucosa tissues and 30 normal colonic mucosa tissues was determined via RT-qPCR. As presented in Fig. 1A, miR-4262 expression was significantly increased in the colonic mucosa tissues from children with IBD compared with the normal tissues. Furthermore, a higher miR-4262 expression was found in the 2% DSS-induced Caco-2 cells compared with the control (Fig. 1B). These data demonstrated that miR-4262 was upregulated in colonic mucosa tissues from patients with IBD and in 2% DSS-stimulated Caco-2 cells and may thus be a regulator of IBD.

Figure 1.

Figure 1

Open in a new tab

miR-4262 is significantly upregulated in colonic mucosa tissues from patients with IBD and DSS-treated Caco-2 cells. (A) The level of miR-4262 in IBD colonic mucosa tissues and normal colonic mucosa tissues was measured via RT-qPCR. (B) RT-qPCR analysis was performed to detect the miR-4262 mRNA levels in 2% DSS-treated Caco-2 cells and a normal intestinal epithelial cell line. The data are presented as the mean ± SD. **P<0.01 vs. non-IBD; ##P<0.01 vs. control. RT-qPCR, reverse transcription-quantitative PCR; miR, microRNA; IBD, inflammatory bowel disease; DSS, dextran sulfate sodium.

Prediction of SIRT1 as a direct target of miR-4262

miRNAs perform biological functions by mediating targets levels (5). Based on TargetScan 7.2 analysis, the role of miR-4262 in IBD was identified. As presented in Fig. 2A, SIRT1 was identified as a potential target of miR-4262. To further verify the hypothesis, a dual luciferase reporter system was conducted to explore the relevance between miR-4262 and SIRT1. Primarily, it was confirmed that miR-4262 mimic significantly enhanced miR-4262 levels in Caco-2 cells (Fig. 2B). A significant suppression in SIRT1 3'-UTR wild-type luciferase activity was observed compared with the mimic control, while SIRT1-MUT luciferase activity exhibited no significant differences (Fig. 2C). In summary, these data confirmed that SIRT1 represents a direct target of miR-4262.

Figure 2.

Figure 2

Open in a new tab

SIRT1 is a target gene of miR-4262. (A) A schematic of miR-4262 target site in SIRT1 3'untranslated region. (B) Expression level of miR-4262 in Caco-2 cells transfected with mimic control or miR-4262 mimic. (C) The dual luciferase reporter assay revealed the combination of miR-4262 mimics/control and WT/MUT SIRT1. The data are presented as the mean ± SD. **P<0.01 vs. mimic control. WT, wild-type; MUT, mutant; miR, micro RNA; SIRT 1, sirtuin 1.

Expression of SIRT1 is suppressed in 2% DSS-stimulated Caco-2 cells and colonic mucosa tissues from patients with IBD

According to the aforementioned resukts, it was speculated that SIRT1 may participate in the progression of IBD. To confirm this assumption, RT-qPCR analysis was performed to detect the SIRT1 levels in colonic mucosa tissues from patients with IBD and in normal colonic mucosa tissues. The results suggested that SIRT1 mRNA expression was markedly decreased in tissues from patients with IBD compared with normal tissues (Fig. 3A). Moreover, the results from RT-qPCR and western blot analysis revealed that SIRT1 was downregulated in the 2% DSS-treated Caco-2 cells compared with the control cells (Fig. 3B and C). Thus, from these data, it can be concluded that SIRT1 participates in the development of IBD.

Figure 3.

Figure 3

Open in a new tab

The expression of SIRT1 is suppressed in colonic mucosa tissues from patients with IBD and in DSS-treated Caco-2 cells. (A) RT-qPCR revealed the SIRT1 mRNA levels in IBD colonic mucosa tissues and normal colonic mucosa tissues. The mRNA levels and protein expression of SIRT1 were determined via (B) RT-qPCR and (C) western blot analysis in normal cells and 2% DSS treated Caco-2 cells. Three independent experiment were conducted, and the data are presented as the mean ± SD. **P<0.01 vs. non-IBD; ##P<0.01 vs. control. RT-qPCR, reverse transcription-quantitative PCR; miR, microRNA; IBD, inflammatory bowel disease; DSS, dextran sulfate sodium; SIRT1, sirtuin 1.

SIRT1-siRNA reverses the effects of miR-4262 inhibitor on SIRT1 expression in Caco-2 cells

Subsequently, it was determined whether miR-4262 affects SIRT1 expression in IBD. Inhibitor control, miR-4262 inhibitor, control-siRNA or SIRT1-siRNA were transfected into Caco-2 cells for 48 h. RT-qPCR analysis suggested that compared with the inhibitor control group, miR-4262 was downregulated in Caco-2 cells following transfection with miR-4262 inhibitor (Fig. 4A). Moreover, as presented in Fig. 4B, compared with the control-siRNA group, SIRT1-siRNA significantly decreased the SIRT1 levels. To further explore the association between miR-4262 and SIRT1, RT-qPCR and western blot analysis were performed to measure SIRT1 expression in the different groups following transfection. Higher mRNA and protein expression levels of SIRT1 were identified in the miR-4262 inhibitor group; however, these effects were partially restored in the miR-4262 inhibitor + SIRT1-siRNA group compared with the miR-4262 inhibitor + control-siRNA group (Fig. 4C and D). These results further confirmed that SIRT1 represents a target gene of miR-4262 and that miR-4262 negatively regulates SIRT1 in IBD cells.

Figure 4.

Figure 4

Open in a new tab

SIRT1-siRNA reverses the effects of miR-4262 inhibitor on SIRT1 expression. Control-siRNA, SIRT1-siRNA, inhibitor control or miR-4262 inhibitor were transfected into Caco-2 cells for 48 h. Following transfection, RT-qPCR was performed to verify the transfection efficiency. (A) mRNA levels of miR-4262 following the transfection of miR-4262 inhibitor and inhibitor control in Caco-2 cells. (B) mRNA level of SIRT1 in Caco-2 cells following the transfection of control-siRNA and SIRT1-siRNA. The (C) mRNA and (D) protein level of SIRT1 were detected via RT-qPCR and western blot analysis, respectively, in Caco-2 cells following transfection with miR-4262 inhibitor + control-siRNA and miR-4262 inhibitor + SIRT1-siRNA. Three independent experiment were conducted, and the data are presented as the mean ± SD. **P<0.01 vs. inhibitor control; ##P<0.01 vs. control-siRNA; &&P<0.01 vs. miR-4262 inhibitor + control-siRNA. RT-qPCR, reverse transcription-quantitative PCR; miR, microRNA; siRNA, small interfering RNA; DSS, dextran sulfate sodium; SIRT1, sirtuin 1.

miR-4262 inhibitor decreases the secretion of inflammatory factors in DSS-stimulated Caco-2 cells via regulating SIRT1

Multiple reports have confirmed that IBD is associated with inflammatory factor secretion (20,21). Thus, the levels of inflammatory cytokines were measured in DSS-treated Caco-2 cells following transfection for 48 h. The results were verified using ELISAs. It was revealed that DSS promoted the release of TNF-α, IL-1β and IL-6 in Caco-2 cells compared with the control group (Fig. 5A-C). In addition, cells cultured with DSS and miR-4262 inhibitor exhibited a lower inflammatory response, verified by the decreased TNF-α, IL-1β and IL-6 expression levels compared with the DSS + inhibitor control group. However, these effects were partially reversed following transfection with SIRT1-siRNA, and it was revealed that the levels of TNF-α, IL-1β and IL-6 were significantly enhanced in the DSS + miR-4246 inhibitor + SIRT1-siRNA group (Fig. 5A-C). The same trends were observed at the mRNA level via RT-qPCR (Fig. 5D-F). The aforementioned results demonstrated that SIRT1-siRNA reversed the inhibitory function of the miR-4262 inhibitor on the expression of inflammatory factors in DSS-treated Caco-2 cells, suggesting that the silencing of SIRT1 may prevent miR-4262 inhibitor from inhibiting the inflammatory response in DSS-induced Caco-2 cells.

Figure 5.

Figure 5

Open in a new tab

SIRT1-siRNA reverses the effects of miR-4262 inhibitor on the secretion of inflammatory factors. Inhibitor control, miR-4262 inhibitor, control-siRNA and SIRT1-siRNA were transfected into Caco-2 cells for 48 h. Then, the cells were stimulated with DSS for 4 days. Cells were divided into 6 groups: Control, DSS, DSS + inhibitor control, DSS + miR-4246 inhibitor, DSS + miR-4246 inhibitor + control-siRNA, DSS + miR-4246 inhibitor + SIRT1-siRNA. (The release of the inflammatory factors (A) TNF-α, (B) IL-1β and (C) IL-6 were detected via ELISAs. The (D) TNF-α, (E) IL-1β and (F) IL-6 mRNA levels were evaluated via reverse transcription-quantitative PCR in 6 groups. Three independent experiment were conducted, and the data are presented as the mean ± SD. *P<0.05, **P<0.01 vs. control; #P<0.05, ##P<0.01 vs. DSS + inhibitor control; &P<0.05, &&P<0.01 vs. DSS + miR-4246 inhibitor + control-siRNA. miR, microRNA; siRNA, small interfering RNA; DSS, dextran sulfate sodium; SIRT1, sirtuin 1; IL, interleukin; TNF, tumor necrosis factor.

miR-4262 inhibitor promotes cell viability and decreases cell apoptosis in DSS-stimulated Caco-2 by regulating SIRT1

To intensively explore the functional mechanism of miR-4262 in IBD, Caco-2 cells were transfected with inhibitor control, miR-4262 inhibitor, control-siRNA or SIRT1-siRNA for 48 h, followed by 2% DSS treatment for 4 days. MTT assays and flow cytometric analysis were conducted to evaluate the Caco-2 cell viability and apoptosis, respectively. As presented in Fig. 6A, DSS treatment markedly inhibited Caco-2 cell viability compared with the control. Moreover, in the DSS-induced cells, miR-4262 inhibitor stimulated cell viability compared to the inhibitor control. However, in the DSS + miR-4246 inhibitor + SIRT1-siRNA group, cell viability was significantly suppressed. As indicated in Fig. 6B and C, it was demonstrated that compared with the control group, DSS significantly induced Caco-2 cell apoptosis, while compared with the DSS + inhibitor control group, transfection with a miR-4246 inhibitor markedly inhibited cell apoptosis; however, this effect was reversed in the DSS + miR-4246 inhibitor + SIRT1-siRNA group compared with the DSS + miR-4246 inhibitor + control-siRNA group.

Figure 6.

Figure 6

Open in a new tab

SIRT1-siRNA eliminates the effects of miR-4262 inhibitor on Caco-2 cell viability and apoptosis. Caco-2 cells were transfected with inhibitor control, miR-4262 inhibitor, control-siRNA and SIRT1-siRNA for 48 h, and the cells were then treated with 2% DSS for 4 days. Cells were divided into 6 groups: Control, DSS, DSS + inhibitor control, DSS + miR-4246 inhibitor, DSS + miR-4246 inhibitor + control-siRNA, DSS + miR-4246 inhibitor + SIRT1-siRNA. (A) MTT was performed to detect Caco-2 cell viability in the 6 groups. (B) FCM was conducted to measure Caco-2 cell apoptosis in the 6 groups. (C) Results of FCM are displayed as a bar graph. Three independent experiment were conducted, and the data are presented as the mean ± SD. **P<0.01 vs. Control; ##P<0.01 vs. DSS + inhibitor control; &&P<0.01 vs. DSS + miR-4246 inhibitor + control-siRNA. miR, microRNA; siRNA, small interfering RNA; DSS, dextran sulfate sodium; SIRT1, sirtuin 1; PI, propidium iodide.

miR-4262 inhibitor suppresses NF-κB pathway activation in DSS-stimulated Caco-2 cells cia regulating SIRT1

The NF-κB pathway has been confirmed to participate in the progression of IBD (22). Finally, western blot analysis was performed to detect the associated protein expression levels of the NF-κB pathway in DSS-induced cells. The results revealed that p-p65 expression was increased (Fig. 7A) and the ratio of p-p65/p65 was enhanced (Fig. 7B) in the DSS-treated cells. However, it was then observed that p-p65 was downregulated and the ratio of p-p65/p65 was decreased in the DSS-treated Caco-2 cells following transfection with a miR-4262 inhibitor. However, in the DSS + miR-4246 inhibitor + SIRT1-siRNA group, the expression of p-p65 was enhanced and the ratio of p-p65/p65 was increased. Collectively, the current results demonstrate that transfection with a miR-4262 inhibitor exerts protective effects on IBD cells by inhibiting both the inflammatory response and Caco-2 cell apoptosis via the regulation of SIRT1, indicating that miR-4262 may represent a potential target for IBD treatment.

Figure 7.

Figure 7

Open in a new tab

miR-4262 inhibitor alleviates the activation of the NF-κB pathway in Caco-2 cells and SIRT1-siRNA reverses the effects. Caco-2 cells were transfected with inhibitor control, miR-4262 inhibitor, control-siRNA and SIRT1-siRNA for 48 h, and DSS was used to induce the cells for 4 days. Cells were divided into 6 groups: Control, DSS, DSS + inhibitor control, DSS + miR-4246 inhibitor, DSS + miR-4246 inhibitor + control-siRNA, DSS + miR-4246 inhibitor + SIRT1-siRNA. (A) Western blot analysis of p65 and p-p65 expression in different groups. (B) Relative protein expression of p-p65/p65 was measured. Three independent experiment were conducted, and the data are presented as the mean ± SD. **P<0.01 vs. control; ##P<0.01 vs. DSS + inhibitor control; &&P<0.01 vs. DSS + miR-4246 inhibitor + control-siRNA. miR, microRNA; siRNA, small interfering RNA; DSS, dextran sulfate sodium; SIRT1, sirtuin 1; p-, phosphorylated.

Discussion

IBD, a chronic and immune disease, is associated with hindering intestinal peristalsis, a shortening colon and inflammation (23). A previous associated study indicated that chronic inflammation is a main risk factor in the progression of colorectal cancer and is involved in the pathogenesis of IBD, while therapies for patients with IBD are limited, where many patients do not respond to or cannot sustain treatment with these drugs, which have various side effects (24). Therefore, the present study aimed to identify a novel target and to explore the underlying mechanisms for IBD treatment. DSS is widely adopted to stimulate colonic inflammation for in vivo and in vitro IBD models (25,26), and in a DSS mouse IBD model, DSS appears to be directly toxic to colonic epithelial cells in the basal crypt (26). Thus, in the present study, DSS was used to treat Caco-2 cells and the effects of DSS on Caco-2 cells and IBD tissues were examined.

It has been previously characterized that miRNAs serve various roles in disease development (27). Moreover, several miRNAs, such as miR-135a have been confirmed to participate in the progression of IBD (28). miR-4262, a recently discovered miRNA, functions as either an oncogene or tumor-suppressor gene in cancers (29). Numerous studies have demonstrated that miR-4262 exhibits an abnormal expression in multiple pathological processes, such as non-small cell lung cancer. For example, Sun et al (30) reported that miR-4262 was involved in paclitaxel resistance via the regulation of PTEN in non-small cell lung cancer. However, the findings by Song et al (31) indicated that the levels of miR-4262 were significantly decreased in osteosarcoma tissues. All these findings suggest that miR-4262 serves a vital role in the development of cancers. However, as far as is known, the specific mechanisms of action of miR-4262 in IBD remain to be clarified. In the current study, the effects of miR-4262 on the progression of IBD were investigated.

In the present study, the level of miR-4262 in IBD colonic mucosa tissues and normal tissues, as well as in 2% DSS-stimulated Caco-2 cells and untreated Caco-2 cells was first determined. It was revealed that the expression of miR-4262 was significantly higher in IBD colonic mucosa tissues compared with normal tissues. Moreover, a significant downregulation of miR-4262 in 2% DSS-stimulated Caco-2 cells was observed, compared with normal cells. These results indicated that miR-4262 may represent a regulator of IBD. Bioinformatics tools and a dual-luciferase reporter assay were then adopted to predict the miR-4262 target gene. The results indicated that miR-4262 directly targeted the SIRT1 3'-UTR and negatively regulated SIRT1 expression levels. It was demonstrated that the level of SIRT1 in IBD colonic mucosa tissues was higher compared with in normal colonic mucosa tissues. The results suggested that SIRT1 mRNA expression was significantly reduced in IBD tissues compared with normal tissues. In addition, the results from RT-qPCR and western blot analysis indicated that SIRT1 was downregulated in the 2% DSS-treated Caco-2 cells compared with the control cells, suggesting that SIRT1 participated in the development of IBD. Based on these data, it was assumed that the altered expression of miR-4262 may affect the development of IBD. Furthermore, whether miR-4262 influences SIRT1 expression in IBD was determined. Inhibitor control, miR-4262 inhibitor, control-siRNA or SIRT1-siRNA were transfected into Caco-2 cells for 48 h. The data revealed that miR-4262 was downregulated in Caco-2 cells following transfection with miR-4262 inhibitor, and SIRT1-siRNA significantly decreased the SIRT1 levels. Moreover, higher mRNA and protein expression level of SIRT1 were obtained in the miR-4262 inhibitor group; however, these effects were abolished in the miR-4262 inhibitor + SIRT1-siRNA group. These results further confirmed that miR-4262 negatively regulates SIRT1 expression via targeting the 3'UTR of SIRT1 in IBD.

Various reports have confirmed that the inflammatory response is a main characteristic of IBD and is highly associated with the progression of IBD (20,21,32). Pro-inflammatory factors, including IL-1β, TNF-α and IL-6 have been confirmed as important regulators in the occurrence of IBD (25). DSS-induced murine and cell models of IBD are often adopted for the investigation of intestinal inflammatory diseases (18,33). To further investigate the underlying mechanisms of miR-4262 in IBD, in the present study, Caco-2 cells were transfected with inhibitor control, miR-4262 inhibitor, control-siRNA or SIRT1-siRNA for 48 h, followed by 2% DSS treatment for 4 days. It was demonstrated that DSS induced a greater production of pro-inflammatory factors in Caco-2 cells, while miR-4262 inhibitor alleviated the release of DSS-stimulated inflammatory factors and that these results were reversed by transfection with SIRT1-siRNA. DSS inhibited Caco-2 cell growth and induced cell apoptosis compared with the control. Meanwhile, miR-4262 inhibitor promoted cell growth and decreased cell apoptosis in DSS-induced Caco-2 cells, and this effect was reversed in the DSS + miR-4246 inhibitor + SIRT1-siRNA group compared with the DSS + miR-4246 inhibitor + control-siRNA group. The data revealed that miR-4246 functions as a marker in IBD progression. These results are consistent with those of previous studies. Sun et al (34) reported that miR-4262 regulated chondrocyte viability, apoptosis and autophagy by targeting SIRT1 and activating the PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Accumulating studies have confirmed the role of NF-κB in inflammatory diseases (35,36). Several reports have demonstrated that miRNAs activate the NF-κB pathway, which may be associated with the development of inflammatory diseases (37,38). Therefore, the present study further validated the effects of NF-κB activity in IBD. Notably, the present findings revealed that p-p65 expression was increased and the ratio of p-p65/p65 was enhanced in DSS-treated Caco-2 cells. Transfection with the miR-4262 inhibitor knocked down p-p65 expression and decreased the ratio of p-p65/p65 in DSS-treated Caco-2 cells following transfection. However, opposite effects were observed in the DSS + miR-4246 inhibitor + SIRT1-siRNA group; the expression of p65 were enhanced and the ratio of p-p65/p65 was increased. These data demonstrated that miR-4246 inhibitor serves a protective role in IBD by alleviating apoptosis and activating NF-κB pathways.

Taken together, the present study, to the best of our knowledge, was the first to demonstrate the role of miR-4262 in the development of IBD. The results of the present study demonstrated the protective effects of transfection with miR-4262 inhibitor on DSS-induced Caco-2 cells, evidenced by inhibiting the inflammatory response, increasing cell growth and decreasing cells apoptosis, via regulation of the miR-4262/SIRT1 axis. This indicates that miR-4262 may represent a potential target for IBD treatment.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

XD contributed to study design, data collection, statistical analysis, data interpretation and manuscript preparation. LS, MD, LY and LX contributed to data collection and statistical analysis. XX contributed to data collection, statistical analysis, and manuscript preparation. All authors read and approved the final manuscript.

Ethics approval and consent to participate

All specimens were obtained with written informed consent and the present study was approved by the Ethics Committee of Chengdu Women's and Children's Central Hospital.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References

  • 1.Mogilevski T, Burgell R, Aziz Q, Gibson PR. Review article: The role of the autonomic nervous system in the pathogenesis and therapy of IBD. Aliment Pharmacol Ther. 2019;50:720–737. doi: 10.1111/apt.15433. [DOI] [PubMed] [Google Scholar]
  • 2.Hammer T, Lophaven SN, Nielsen KR, von Euler-Chelpin M, Weihe P, Munkholm P, Burisch J, Lynge E. Inflammatory bowel diseases in Faroese-born Danish residents and their offspring: Further evidence of the dominant role of environmental factors in IBD development. Aliment Pharmacol Ther. 2017;45:1107–1114. doi: 10.1111/apt.13975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Wolf DC, Abraham BP, Afzali A, Allegretti PD, Arai R. Community perspectives: Combining serology, genetics, and inflammation markers for the diagnosis of IBD and differentiation between CD and UC. Gastroenterol Hepatol (N Y) 2012;8:S1–S16. [PMC free article] [PubMed] [Google Scholar]
  • 4.Liu R, Tang A, Wang X, Chen X, Zhao L, Xiao Z, Shen S. Inhibition of lncRNA NEAT1 suppresses the inflammatory response in IBD by modulating the intestinal epithelial barrier and by exosome-mediated polarization of macrophages. Int J Mol Med. 2018;42:2903–2913. doi: 10.3892/ijmm.2018.3829. [DOI] [PubMed] [Google Scholar]
  • 5.Pereira TDSF, Brito JAR, Guimarães ALS, Gomes CC, de Lacerda JCT, de Castro WH, Coimbra RS, Diniz MG, Gomez RS. MicroRNA profiling reveals dysregulated microRNAs and their target gene regulatory networks in cemento-ossifying fibroma. J Oral Pathol Med. 2018;47:78–85. doi: 10.1111/jop.12650. [DOI] [PubMed] [Google Scholar]
  • 6.Wu ZW, Liu YF, Wang S, Li B. miRNA-146a induces vascular smooth muscle cell apoptosis in a rat model of coronary heart disease via NF-κB pathway. Genet Mol Res. 2015;14:18703–18712. doi: 10.4238/2015.December.28.19. [DOI] [PubMed] [Google Scholar]
  • 7.Wang J, Yin K, Lv X, Yang Q, Shao M, Liu X, Sun H. MicroRNA-24-3p regulates Hodgkin's lymphoma cell proliferation, migration and invasion by targeting DEDD. Oncol Lett. 2019;17:365–371. doi: 10.3892/ol.2018.9599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Chen Y, Du J, Zhang Z, Liu T, Shi Y, Ge X, Li YC. MicroRNA-346 mediates tumor necrosis factor α-induced downregulation of gut epithelial vitamin D receptor in inflammatory bowel diseases. Inflamm Bowel Dis. 2014;20:1910–1918. doi: 10.1097/MIB.0000000000000158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Huang L, Ma Q, Li Y, Li B, Zhang L. Inhibition of microRNA-210 suppresses pro-inflammatory response and reduces acute brain injury of ischemic stroke in mice. Exp Neurol. 2018;300:41–50. doi: 10.1016/j.expneurol.2017.10.024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Kumar A, Bhatia HS, de Oliveira AC, Fiebich BL. microRNA-26a modulates inflammatory response induced by toll-like receptor 4 stimulation in microglia. J Neurochem. 2015;135:1189–1202. doi: 10.1111/jnc.13364. [DOI] [PubMed] [Google Scholar]
  • 11.Wang K, Ren Y, Liu Y, Zhang J, He JJ. miR-4262 promotes proliferation and invasion of human breast cancer cells through directly targeting KLF6 and KLF15. Oncol Res. 2017;25:277–283. doi: 10.3727/096504016X14732514133203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Weng L, Ma J, Jia YP, Wu SQ, Liu BY, Cao Y, Yin X, Shang MY, Mao AW. miR-4262 promotes cell apoptosis and inhibits proliferation of colon cancer cells: Involvement of GALNT4. Am J Transl Res. 2018;10:3969–3977. [PMC free article] [PubMed] [Google Scholar]
  • 13.Zhang D, Li Z, Zhang Y, Tu C, Huo J, Liu Y. miR-4262 promotes the proliferation of human cutaneous malignant melanoma cells through KLF6-mediated EGFR inactivation and p21 upregulation. Oncol Rep. 2016;36:3657–3663. doi: 10.3892/or.2016.5190. [DOI] [PubMed] [Google Scholar]
  • 14.Yang L, Duan Z, Liu X, Yuan Y. N-acetyl-l-cysteine ameliorates the PM2.5-induced oxidative stress by regulating SIRT-1 in rats. Environ Toxicol Pharmacol. 2018;57:70–75. doi: 10.1016/j.etap.2017.11.011. [DOI] [PubMed] [Google Scholar]
  • 15.Sim WC, Kim DG, Lee W, Sim H, Choi YJ, Lee BH. Activation of SIRT1 by L-serine increases fatty acid oxidation and reverses insulin resistance in C2C12 myotubes. Cell Biol Toxicol. 2019;35:457–470. doi: 10.1007/s10565-019-09463-x. [DOI] [PubMed] [Google Scholar]
  • 16.Pan Q, Lou X, Zhang J, Zhu Y, Li F, Shan Q, Chen X, Xie Y, Su S, Wei H, et al. Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer. Sci Rep. 2017;7(25) doi: 10.1038/s41598-017-00057-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Menconi A, Hernandez-Velasco X, Vicuña EA, Kuttappan VA, Faulkner OB, Tellez G, Hargis BM, Bielke LR. Histopathological and morphometric changes induced by a dextran sodium sulfate (DSS) model in broilers. Poult Sci. 2015;94:906–911. doi: 10.3382/ps/pev054. [DOI] [PubMed] [Google Scholar]
  • 18.Nighot P, Young K, Nighot M, Rawat M, Sung EJ, Maharshak N, Plevy SE, Ma T, Blikslager A. Chloride channel ClC-2 is a key factor in the development of DSS-induced murine colitis. Inflamm Bowel Dis. 2013;19:2867–2877. doi: 10.1097/MIB.0b013e3182a82ae9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25:402–408. doi: 10.1006/meth.2001.1262. [DOI] [PubMed] [Google Scholar]
  • 20.Friedrich M, Pohin M, Powrie F. Cytokine networks in the pathophysiology of inflammatory bowel disease. Immunity. 2019;50:992–1006. doi: 10.1016/j.immuni.2019.03.017. [DOI] [PubMed] [Google Scholar]
  • 21.Rogler G. Resolution of inflammation in inflammatory bowel disease. Lancet Gastroenterol Hepatol. 2017;2:521–530. doi: 10.1016/S2468-1253(17)30031-6. [DOI] [PubMed] [Google Scholar]
  • 22.McDaniel DK, Eden K, Ringel VM, Allen IC. Emerging roles for noncanonical NF-κB signaling in the modulation of inflammatory bowel disease pathobiology. Inflamm Bowel Dis. 2016;22:2265–2279. doi: 10.1097/MIB.0000000000000858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Soufflet F, Biraud M, Rolli-Derkinderen M, Lardeux B, Trang C, Coron E, Bruley des Varannes S, Bourreille A, Neunlist M. Modulation of VIPergic phenotype of enteric neurons by colonic biopsy supernatants from patients with inflammatory bowel diseases: Involvement of IL-6 in Crohn's disease. Neurogastroenterol Motil. 2018;30 doi: 10.1111/nmo.13198. [DOI] [PubMed] [Google Scholar]
  • 24.Schenk M, Bouchon A, Seibold F, Mueller C. TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J Clin Invest. 2007;117:3097–3106. doi: 10.1172/JCI30602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Zhao Q, Liu Y, Tan L, Yan L, Zuo X. Adiponectin administration alleviates DSS-induced colonic inflammation in Caco-2 cells and mice. Inflamm Res. 2018;67:663–670. doi: 10.1007/s00011-018-1155-6. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  • 26.Wirtz S, Neufert C, Weigmann B, Neurath MF. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007;2:541–546. doi: 10.1038/nprot.2007.41. [DOI] [PubMed] [Google Scholar]
  • 27.Liu X, Chen J, Guan T, Yao H, Zhang W, Guan Z, Wang Y. Correction to: miRNAs and target genes in the blood as biomarkers for the early diagnosis of Parkinson's disease. BMC Syst Biol. 2019;13(20) doi: 10.1186/s12918-019-0680-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Zhang J, Lian B, Shang Y, Li C, Meng Q. miR-135a protects dextran sodium sulfate-induced inflammation in human colorectal cell lines by activating STAT3 signal. Cell Physiol Biochem. 2018;51:1001–1012. doi: 10.1159/000495481. [DOI] [PubMed] [Google Scholar]
  • 29.Zhang H, Jiang H, Zhang H, Liu J, Hu X, Chen L. miR-4262, low level of which predicts poor prognosis, targets proto-oncogene CD163 to suppress cell proliferation and invasion in gastric cancer. Onco Targets Ther. 2019;12:599–607. doi: 10.2147/OTT.S187881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Sun H, Zhou X, Bao Y, Xiong G, Cui Y, Zhou H. Involvement of miR-4262 in paclitaxel resistance through the regulation of PTEN in non-small cell lung cancer. Open Biol. 2019;9(180227) doi: 10.1098/rsob.180227. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  • 31.Song K, Liu N, Yang Y, Qiu X. Regulation of osteosarcoma cell invasion through osteopontin modification by miR-4262. Tumour Biol. 2016;37:6493–6499. doi: 10.1007/s13277-015-4530-8. [DOI] [PubMed] [Google Scholar]
  • 32.Gerasimidis K, Edwards C, Stefanowicz F, Galloway P, McGrogan P, Duncan A, Talwar D. Micronutrient status in children with IBD: True deficiencies or epiphenomenon of the systemic inflammatory response. J Pediatr Gastroenterol Nutr. 2013;56:e50–e51. doi: 10.1097/MPG.0b013e31828f1e86. [DOI] [PubMed] [Google Scholar]
  • 33.Eichele DD, Kharbanda KK. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol. 2017;23:6016–6029. doi: 10.3748/wjg.v23.i33.6016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Sun W, Li Y, Wei S. miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Exp Ther Med. 2018;15:1119–1128. doi: 10.3892/etm.2017.5444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Schuliga M. NF-kappaB signaling in chronic inflammatory airway disease. Biomolecules. 2015;5:1266–1283. doi: 10.3390/biom5031266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Lin TH, Pajarinen J, Lu L, Nabeshima A, Cordova LA, Yao Z, Goodman SB. NF-κB as a therapeutic target in inflammatory-associated bone diseases. Adv Protein Chem Struct Biol. 2017;107:117–154. doi: 10.1016/bs.apcsb.2016.11.002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Hill JM, Clement C, Zhao Y, Lukiw WJ. Induction of the pro-inflammatory NF-kB-sensitive miRNA-146a by human neurotrophic viruses. Front Microbiol. 2015;6(43) doi: 10.3389/fmicb.2015.00043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Pogue AI, Percy ME, Cui JG, Li YY, Bhattacharjee S, Hill JM, Kruck TP, Zhao Y, Lukiw WJ. Up-regulation of NF-kB-sensitive miRNA-125b and miRNA-146a in metal sulfate-stressed human astroglial (HAG) primary cell cultures. J Inorg Biochem. 2011;105:1434–1437. doi: 10.1016/j.jinorgbio.2011.05.012. [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Articles from Experimental and Therapeutic Medicine are provided here courtesy of Spandidos Publications

RESOURCES