Table 2.

RT-qPCR thermal cycling conditions.

Step	CDC10	Georgia Tech
(1) UNG incubation	25°C, 2 min	----
(2) Reverse transcription	50°C, 15 min	50°C, 15 min
(3) RT inactivation and/or DNA polymerase activation	95°C, 2 min	95°C, 5 min
(4) Denaturation	95°C, 3 s	95°C, 15 s
(5) Annealing and extension (fluorescence collection) Number cycles of steps 4 and 5	55°C, 30 s 45	55°C, 30 s 45