Table 1. Sequences of CDC primers and probes.
FAM was substituted for HEX in RP probe.
| Gene target | Primer Name | Sequence and probe/quencher label (bold)1 |
|---|---|---|
| N (viral) | 2019-nCoV_N1-P | FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 |
| 2019-nCoV_N1-F | GACCCCAAAATCAGCGAAAT | |
| 2019-nCoV_N1-R | TCTGGTTACTGCCAGTTGAATCTG | |
| 2019-nCoV_N2-P | FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1 | |
| 2019-nCoV_N2-F | TTACAAACATTGGCCGCAAA | |
| 2019-nCoV_N2-R | GCGCGACATTCCGAAGAA | |
| RNase P (human) | RP-P-FAM | FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ1 |
| RP-P-HEX2 | HEX-TTCTGACCTGAAGGCTCTGCGCG-BHQ1 | |
| RP-F | AGATTTGGACCTGCGAGCG | |
| RP-R | GAGCGGCTGTCTCCACAAGT |
1
FAM= fluorescein phosphoramidite, BHQ1= Black Hole Quencher-1, HEX = 5’-hexachloro-fluorescein phosphoramidite
2
Substitute RP-P-HEX for RP-P-FAM in the multiplex reaction.