Figure 6.

Yeast strains with humanized cytoskeletal components exhibit distinct cellular morphologies. (A) Humanized strains show varying cell sizes. Cell areas of humanized strains (in square pixels) are plotted on the X-axis. Gray violins indicate cell size distributions. Orange dots indicate outliers. Humanized actin strains show reduced cell size while myosins and tubulins (except γ-tubulin) largely remain unchanged. However, humanized septin strains show drastically elongated cellular morphologies. Significance comparisons with wild type determined by standard t-test with ***P ≤ 0.001; **0.001 < P ≤ 0.01; *0.01 < P ≤ 0.05; NS, P > 0.05, not significant. Additional bright-field images are shown in Figure S8. (B) Magnified bright-field and DAPI-stained images of humanized septin strains exhibit elongated morphologies and are multinucleated as a consequence of defective cytokinesis. (Scale bar, 10 µm.)