Table 7.
Statistical test | # cell property | Cell property | ANOVA | Tukey’s posthoc t test | ||||
---|---|---|---|---|---|---|---|---|
F(2,76) | p value | R2 | PV vs SST | PV vs VIP | SST vs VIP | |||
ANOVA and Tukey’spost hoc t test | 1 | RMP (mV) | 9.00 | 3.2E-04 | 0.20 | 2.5E-02 | 1.9E-04 | 2.1E-01 |
4 | ct: AHP amplitude (mV) | 28.1 | 8.3E-10 | 0.43 | 4.6E-01 | 2.0E-08 | 1.8E-07 | |
9 | ct: AP threshold (mV) | 7.76 | 8.8E-04 | 0.17 | 2.6E-03 | 1.7E-03 | 9.9E-01 | |
10 | ct: AP amplitude (mV) | 2.75 | 7.0E-02 | 0.07 | 6.4E-01 | 6.6E-02 | 2.9E-01 | |
12 | ct: AP threshold/AP amp | 28.1 | 8.2E-10 | 0.43 | 1.5E-06 | 5.7E-09 | 1.2E-01 | |
16 | 2xct: AHP amplitude (mV) | 11.4 | 5.0E-05 | 0.23 | 4.3E-01 | 1.3E-02 | 4.0E-05 | |
17 | 2xct: AHP latency (ms) | 62.4 | 1.3E-16 | 0.63 | 5.3E-09 | 5.1E-09 | 8.8E-04 | |
20 | 2xct: AP half-width (ms) | 81.5 | 2.0E-19 | 0.69 | 7.5E-08 | 5.1E-09 | 7.6E-09 | |
21 | 2xct: AP threshold (mV) | 25.2 | 4.6E-09 | 0.40 | 1.4E-07 | 2.3E-08 | 8.9E-01 | |
24 | 2xct: AP thresh/AP ampl | 44.2 | 2.3E-13 | 0.54 | 7.7E-09 | 5.1E-09 | 5.6E-02 | |
41 | Max AP: AHP amplitude (mV) | 5.70 | 5.0E-03 | 0.13 | 1.1E-01 | 6.2E-01 | 3.9E-03 | |
42 | Max AP: AHP latency (ms) | 70.6 | 7.1E-18 | 0.66 | 1.9E-08 | 5.1E-09 | 6.2E-07 | |
53 | Max AP: amp 1st /last AP | 2.97 | 5.7E-02 | 0.07 | 5.5E-01 | 5.1E-02 | 3.1E-01 | |
55 | Max AP: adaptation (Hz) | 3.18 | 4.8E-02 | 0.08 | 1.1E-01 | 4.9E-02 | 9.3E-01 | |
Statistical test | # cell property | Cell property | Kruskal–Wallistest | Dunn’s multiplecomparison | ||||
H value | p value | η2 | PV vs SST | PV vs VIP | SST vs VIP | |||
2 | Rm (MΩ) | 51.8 | 5.8E-12 | 0.68 | 7.4E-05 | 1.9E-12 | 3.4E-03 | |
3 | ct (pA) | 53.5 | 2.4E-12 | 0.70 | 6.8E-05 | 8.0E-13 | 2.3E-03 | |
5 | ct: AHP latency (ms) | 43.9 | 2.9E-10 | 0.58 | 1.2E-06 | 3.1E-10 | 4.3E-01 | |
6 | ct: max AP rise (mV/ms) | 25.5 | 2.9E-06 | 0.34 | 1.1E-03 | 1.7E-06 | 3.6E-01 | |
Kruskal–Wallis:Dunn's multiplecomparison test | 7 | ct: max AP decay (mV/ms) | 54.2 | 1.7E-12 | 0.71 | 2.0E-04 | 6.5E-13 | 7.2E-04 |
8 | ct: AP half-width (ms) | 56.6 | 5.1E-13 | 0.74 | 3.8E-04 | 2.3E-13 | 1.8E-04 | |
11 | ct: AP rise/decay ratio | 45.8 | 1.2E-10 | 0.60 | 6.1E-05 | 4.2E-11 | 2.0E-02 | |
13 | ct: AP rise/AP half-width | 47.2 | 5.6E-11 | 0.62 | 2.8E-04 | 2.0E-11 | 3.6E-03 | |
14 | 2xct: # AP | 44.0 | 2.8E-10 | 0.58 | 8.9E-06 | 1.4E-10 | 1.2E-01 | |
15 | 2xct: latency to first AP | 38.9 | 3.5E-09 | 0.51 | 2.6E-07 | 2.1E-08 | 1.0E+00 | |
18 | 2xct: max AP rise (mV/ms) | 29.4 | 4.2E-07 | 0.39 | 5.6E-04 | 2.2E-07 | 2.3E-01 | |
19 | 2xct: max AP decay (mV/ms) | 52.7 | 3.7E-12 | 0.69 | 1.3E-04 | 1.3E-12 | 1.6E-03 | |
22 | 2xct: AP amplitude (mV) | 1.23 | 5.4E-01 | 0.02 | 1.0E+00 | 1.0E+00 | 1.0E+00 | |
23 | 2xct: AP rise/decay ratio | 46.0 | 1.0E-10 | 0.61 | 1.1E-04 | 3.5E-11 | 1.1E-02 | |
25 | 2xct: AP rise/AP half-width | 43.8 | 3.1E-10 | 0.58 | 5.9E-04 | 1.1E-10 | 4.6E-03 | |
26 | 2xct: amp 1st/2nd AP | 17.6 | 1.5E-04 | 0.23 | 1.0E+00 | 1.3E-02 | 1.9E-04 | |
27 | 2xct: amp 2nd/3rd AP | 2.20 | 3.3E-01 | 0.03 | 1.0E+00 | 1.0E+00 | 1.0E+00 | |
28 | 2xct: amp 1st /last AP | 7.31 | 2.6E-02 | 0.10 | 1.0E+00 | 6.9E-02 | 6.4E-02 | |
29 | 2xct: initial instantaneous frequency | 40.1 | 1.9E-09 | 0.53 | 3.6E-08 | 4.9E-08 | 1.0E+00 | |
30 | 2xct: adaptation (Hz) | 15.8 | 3.7E-04 | 0.21 | 2.2E-04 | 1.0E-01 | 1.0E-01 | |
31 | 2xct: ISI ratio | 19.2 | 6.7E-05 | 0.25 | 1.0E+00 | 2.5E-04 | 1.7E-03 | |
32 | 2xct: mean of first 2 ISI (ms) | 41.2 | 1.2E-09 | 0.54 | 3.7E-08 | 2.0E-08 | 1.0E+00 | |
33 | 2xct: SD of first 2 ISI (ms) | 23.2 | 9.1E-06 | 0.31 | 3.5E-03 | 4.8E-06 | 2.8E-01 | |
34 | 2xct: change of ISI duration from 1st to 2nd AP (ms) | 10.6 | 5.0E-03 | 0.14 | 7.3E-03 | 1.7E-02 | 1.0E+00 | |
35 | 2xct: change of instant frequency from 1st to 2nd AP pair (Hz) | 10.7 | 4.8E-03 | 0.14 | 4.3E-03 | 3.7E-02 | 1.0E+00 | |
36 | 2xct: Cv2:all | 34.5 | 3.2E-08 | 0.45 | 1.7E-01 | 7.0E-08 | 1.4E-04 | |
Kruskal–Wallis: Dunn's multiplecomparison test | 37 | 2xct: Cv2-1st AP | 35.0 | 2.5E-08 | 0.46 | 1.8E-01 | 6.1E-08 | 1.1E-04 |
38 | 2xct: Cv2-1st/2nd AP | 35.3 | 2.1E-08 | 0.46 | 2.3E-01 | 6.7E-08 | 6.9E-05 | |
39 | Max AP: # AP | 52.5 | 3.9E-12 | 0.69 | 7.9E-05 | 1.3E-12 | 2.6E-03 | |
40 | Max AP: Latency to first AP | 40.8 | 1.4E-09 | 0.54 | 1.6E-05 | 7.6E-10 | 1.7E-01 | |
43 | Max AP: max AP rise (mV/ms) | 29.9 | 3.2E-07 | 0.39 | 2.2E-04 | 2.0E-07 | 3.7E-01 | |
44 | max AP: max AP decay (mV/ms) | 52.1 | 4.8E-12 | 0.69 | 2.7E-04 | 1.8E-12 | 9.5E-04 | |
45 | Max AP: AP half-width (ms) | 55.2 | 1.0E-12 | 0.73 | 3.1E-03 | 9.0E-13 | 2.9E-05 | |
46 | Max AP: AP threshold (mV) | 29.7 | 3.5E-07 | 0.39 | 5.4E-05 | 4.0E-07 | 9.0E-01 | |
47 | Max AP: AP amplitude (mV) | 2.53 | 2.8E-01 | 0.03 | 1.0E+00 | 1.0E+00 | 1.0E+00 | |
48 | Max AP: AP rise/decay ratio | 48.2 | 3.3E-11 | 0.63 | 6.0E-04 | 1.3E-11 | 1.3E-03 | |
49 | Max AP: AP thresh/AP ampl | 39.0 | 3.4E-09 | 0.51 | 1.8E-04 | 1.4E-09 | 4.6E-02 | |
50 | Max AP: AP rise/ AP half-width | 42.7 | 5.3E-10 | 0.56 | 4.6E-04 | 2.0E-10 | 7.9E-03 | |
51 | Max AP: 1st/2nd AP ratio | 10.4 | 5.6E-03 | 0.14 | 8.1E-01 | 6.2E-03 | 8.5E-02 | |
52 | Max AP: 2nd/3rd AP ratio | 1.79 | 4.1E-01 | 0.02 | 1.0E+00 | 1.0E+00 | 1.0E+00 | |
54 | Max AP: initial instantaneous frequency | 38.8 | 3.7E-09 | 0.51 | 5.8E-06 | 3.6E-09 | 4.9E-01 | |
56 | Max AP: ISI ratio | 18.3 | 1.1E-04 | 0.24 | 5.5E-03 | 7.3E-05 | 7.1E-01 | |
57 | Max AP: Mean of first 2 ISI (ms) | 42.9 | 4.7E-10 | 0.57 | 2.9E-05 | 2.0E-10 | 6.8E-02 | |
58 | Max AP: SD of first 2 ISI (ms) | 49.2 | 2.1E-11 | 0.65 | 2.9E-03 | 1.3E-11 | 2.0E-04 | |
59 | Max AP: change of ISI duration from 1st to 2nd AP (ms) | 25.0 | 3.8E-06 | 0.33 | 1.3E-02 | 1.8E-06 | 5.9E-02 | |
60 | Max AP: change of instant frequency from 1st to 2nd AP pair (Hz) | 8.02 | 1.8E-02 | 0.11 | 2.0E-02 | 9.8E-01 | 1.5E-01 | |
61 | Max AP: Cv2:all | 42.0 | 7.5E-10 | 0.55 | 7.9E-04 | 2.9E-10 | 5.9E-03 | |
62 | Max AP: Cv2-1st AP | 39.7 | 2.4E-09 | 0.52 | 6.9E-04 | 9.1E-10 | 1.3E-02 | |
63 | Max AP: Cv2-1st/2nd AP | 38.5 | 4.3E-09 | 0.51 | 7.2E-04 | 1.6E-09 | 1.7E-02 | |
Sum of significant difference cell properties | 46 | 53 | 30 |
If a cell property showed a normal distribution, ANOVA and Tukey’s post hoc t test was used to compare group means. If a cell property showed a non-normal distribution, a Kruskal–Wallis and Dunn’s multiple comparison test was used to compare group medians.