Table 1.
The concentration of coumestrol and 4′-methoxycoumestrol (mg kg−1) at physiological maturity in selected annual pasture legume species.
| Tissue Type | Pasture Species | Coumestrol | 4′-Methoxycoumestrol | Total Coumestans |
|---|---|---|---|---|
| Leaf | Arrowleaf clover | 14.7 ± 2.2 | 5.9 ± 1.5 | 20.6 ± 5.1 |
| Biserrula cv.* Casbah | 13.1 ± 2.7 | 5.2 ± 1.7 | 18.2 ± 6.5 | |
| Biserrula cv. Mauro | 14.6 ± 2.4 | 7.4 ± 5.5 | 22.0 ± 4.2 | |
| Bladder clover | 48.4 ± 10.6 | 24.8 ± 17.7 | 73.2 ± 13.6 | |
| French serradella | ND | ND | ND | |
| Gland clover | 27.1 ± 11.1 | 21.1 ± 5.6 | 48.2 ± 3.5 | |
| Lucerne | 31.8 ± 14.4 | 20.2 ± 7.5 | 52.0 ± 6.7 | |
| Subterranean clover | 25.1 ± 8.6 | 12.5 ± 4.5 | 37.6 ± 7.3 | |
| Yellow serradella | ND | ND | ND | |
| LSD (p < 0.05) | 9.3 | 5.4 | 13.1 | |
| Stem | Arrowleaf clover | 24.2 ± 5.3 | 6.1 ± 4.6 | 30.3 ± 10.5 |
| Biserrula cv. Casbah | ND | ND | ND | |
| Biserrula cv. Mauro | ND | ND | ND | |
| Bladder clover | 39.6 ± 7.7 | 18.7 ± 3.6 | 58.3 ± 12.1 | |
| French serradella | ND | ND | ND | |
| Gland clover | 26.2 ± 14.4 | 3.6 ± 1.9 | 29.8 ± 13.0 | |
| Lucerne | 36.6 ± 16.5 | 27.7 ± 12.8 | 64.3 ± 5.1 | |
| Subterranean clover | 25.4 ± 7.5 | 7.1 ± 1.6 | 32.5 ± 10.6 | |
| Yellow serradella | ND | ND | ND | |
| LSD (p < 0.05) | 9.4 | 14.5 | 17.5 | |
| Inflorescence | Arrowleaf clover | ND | 0.1 ± 0.1 | <0.1 |
| Biserrula cv. Casbah | ND | ND | ND | |
| Biserrula cv. Mauro | ND | 0.2 ± 0.1 | <0.1 | |
| Bladder clover | 0.2 ± 0.2 | <0.1 | <0.1 | |
| French serradella | ND | ND | ND | |
| Gland clover | 0.1 | ND | <0.1 | |
| Lucerne | 0.5 ± 0.1 | 0.2 ± 0.1 | 0.7 ± 0.1 | |
| Subterranean clover | 0.3 ± 0.1 | ND | 0.3 ± 0.1 | |
| Yellow serradella | ND | ND | ND | |
| LSD (p < 0.05) | 0.2 | 0.1 | 0.2 |
ND denotes compounds not detected. Least significant difference (LSD) differentiated between concentration means. Values represent the arithmetic mean of five replicates ± standard deviation. *cv. is acronym for cultivar.