Figure 2.

Categorization of methods for preparing protein nanoparticles. (a) The chemical method produces protein nanoparticles using a chemical reaction and includes emulsion and complex coacervation. (b) The physical method includes physically aggregating after separating proteins into nanosized particles and includes an electrospray technique and a nano spray drying method. (c) The self-assembly method is a method of making nanoparticles by agglutinating proteins by themselves and it includes the desolvation method.