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. 2020 Jul 10;12(7):650. doi: 10.3390/pharmaceutics12070650

Figure 7.

Figure 7

Detection and quantification of vimentin and phospho-paxillin (red) by immunofluorescence confocal microscopy in fixed CoLo-205 (A–C) and CaCo-2 cells (D–F), after cells incubation with free 5-FU, 5-FU/LPs or anti-FZD10/5-FU/LPs at 24 and 48 h. 5-FU concentration: 2 µM. CTR: untreated cells. Green channel: labeled vimentin, red channel: labeled phospho-paxillin, blue channel: labeled nuclei (DAPI), and corresponding overlay (Merge). Scale bar: 50 µm. (*) p < 0.001 versus control (CRT).