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. 2020 Jul 20;9(7):1733. doi: 10.3390/cells9071733

Figure 3.

Figure 3

Modelling Adult Human Heart Organoids. (A) Generation of contractile cardiac organoids (Cor-Oids) containing Cor.4U-CMs, HCMECs and HCF at 3:5:2 cell ratio using Terasaki plates showed the formation of compact ball-like shapes after 21 days of culture. (B) Immunofluorescence staining images of Cor-Oids after 14 days of culture, labelled with cardiac-specific troponin (TNNT2), vascular cadherin (VeCADH) and vimentin (VIM), showing organoids with a lack of defined cardiac structures (T-tubules and sarcomeres). (C) Immunofluorescence staining images of Cor-Oids after 21 days of culture, labelled with cardiac-specific troponin (TNNT2), vascular cadherin (VeCADH) and vimentin (VIM), showing CMs migration toward the organoid core, promoting the formation of cardiac filament-like structures. Scale bars = 200 µm.