Table 1.
Strains and plasmids used in this study. P3M, Proteus vulgaris strain 3M.
| Strains or Plasmids a | Relevant Characteristics | Source or Reference |
|---|---|---|
| Strains | ||
| P3M | Wild-type strain containing p3M-2A and p3M-2B plasmids | This study |
| P3M-Δ2A | p3M-2A-deficient strain | This study |
| P3M-Δ2B | p3M-2B-deficient strain | This study |
| P3M-Δ2AΔ2B | p3M-2A- and p3M-2B-deficient strain | This study |
| P3M-Δ2A/2A* | p3M-2A-deficient strain complemented with p3M-2A* | This study |
| P3M-Δ2B/2B* | p3M-2B-deficient strain complemented with p3M-2B* | This study |
| Escherichia coli DH5α | Competent cell for cloning b | CWBIO Company (CW0808S) |
| DH5α-2A | DH5α complemented with p3M-2A | This study |
| DH5α-2B | DH5α complemented with p3M-2B | This study |
| DH5α-2A2B | DH5α complemented with p3M-2A and p3M-2B | This study |
| DH5α-2B/2A* | DH5α-2B complemented with p3M-2A* | This study |
| DH5α-2A/2B* | DH5α-2A complemented with p3M-2B* | This study |
| E. coli S17-1 | Mobilizing donor strain with streptomycin resistance | [30] |
| Plasmids | ||
| p3M-2A | 2656-bp plasmid isolated from P3M | This study |
| p3M-2B | 5903-bp plasmid carrying qnrD, isolated from P3M | This study |
| p3M-2A* | p3M-2A without ORF1 | This study |
| p3M-2B* | p3M-2B without ORF5 | This study |
a The asterisks (*) denote deletion of ORF1 from p3M-2A or deletion of ORF5 from p3M-2B. b The gyrA mutation in E. coli DH5α does not increase the minimum inhibitory concentration (MIC) for ciprofloxacin.