Figure 3.

Coxsackieviral RNA in the T1D pancreas. Representative images of T1D donors 6070 and 6211 from the nPOD cohort. Viral RNA was found within the endocrine area (A) and outside the islets (B,C) shown by co-staining of viral RNA probes (red), insulin (green) and DAPI (nuclei; blue). Tissues were first probed for viral RNA, and then stained for insulin after a previously established protocol ([77]; Busse et al.). Scale bar depicts 10 µm.