Determination of the impact of SS-derived fractions on IAV proteins. (A–F) DMSO control and the SS-derived fractions were added to solutions containing purified IAV; reactions proceeded at 25 °C for 48 h. Aqu: Aqueous extract. (A) The viral titer of each reaction was evaluated. Error bars indicate mean ± SD; n = 3 per group. (B) The images to the left and right are the results of CBB staining of SDS-samples without 2-Me and with 2-Me, respectively. M: Marker. (C) The images to the left and right are the results of WB to detect IAV HA proteins. (D) The HA titer was evaluated. Error bars indicate mean ± SD; n = 3 per group. (E) The image is the result of WB to detect IAV NA protein. (F) The NA activity was evaluated. Error bars indicate mean of triplicate measurement of same sample ± SD. (A,D) Student’s t test was performed to analyze statistical difference; ** p < 0.01, *** p < 0.001. (B,C,E,F) The results are representatives of two individual experiments.