Skip to main content
. 2020 Jul 13;10(20):9132–9152. doi: 10.7150/thno.46076

Figure 6.

Figure 6

(A) Confocal microscope images of macrophages with M0 (monocyte), M1, and M2 phenotype upon IL-4 and LPS/IFN-γ stimulation, and immunostaining with FITC-anti-CD86 or PE-anti-CD206. Green: FITC (Ex: 488 nm, Em: 510 nm); red: PE (Ex: 543 nm, Em: 580 nm). Scale bars: 50 µm; (B) FCM analysis of the reprogramed M1-type macrophages from M2-type (TAMs) after incubation with CC and MCC; (C) Scheme of inhibition of 4T1 tumor cells after incubation with TAMs upon CC or MCC stimulation; (D) Cell viability of 4T1 tumor cells co-cultured with M1-type TAMs or M2-type TAMs; (E) Cell viability of 4T1 tumor cells during post-PDT period co-cultured with TAMs incubated with CC or MCC. Quantitative cytokine analysis of pro-tumor IL-10 (F), anti-tumor IL-6 (G), and TNF-α (H) in different groups.