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. 2020 Jul 23;10(20):9303–9314. doi: 10.7150/thno.46568

Figure 3.

Figure 3

Identification of the five proteins on HepG2 Exos by immunoblotting assays. (A) Representative TEM images of HepG2 Exos labeled with different immuno-gold antibodies: Anti-CD63, anti-CEA, anti-GPC-3, anti-PD-L1, and anti-HER2. A mixture of Exos and pure AuNPs was used as a control. The labeled AuNPs had a mean size of 8 nm. Scale bar: 50 nm. (B) Determination of the five exosomal biomarkers by Western-blot analysis. Each group was loaded with the same quantity of total denatured proteins (15 µg). (C) Dot blotting analysis of the tested biomarkers on native Exos and Exo@Au nanozymes without protein denaturation. PEG-stabilized AuNPs (2 nm) were used as a control.