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. 2020 Aug 13;11(8):617. doi: 10.1038/s41419-020-02845-8

Costello syndrome model mice with a HrasG12S/+ mutation are susceptible to develop house dust mite-induced atopic dermatitis

Yu Katata 1,2, Shin-ichi Inoue 1, Atsuko Asao 3, Shuhei Kobayashi 3,4, Hitoshi Terui 5, Aya Inoue-Shibui 1, Taiki Abe 1, Tetsuya Niihori 1, Setsuya Aiba 5, Naoto Ishii 3, Shigeo Kure 2, Yoko Aoki 1,✉
PMCID: PMC7426869  PMID: 32792500

Abstract

Costello syndrome is an autosomal dominant disorder that is caused by germline HRAS mutations. Patients with Costello syndrome present craniofacial abnormalities, cardiac defects, and cancer predisposition, as well as skin abnormalities, including papillomas, keratosis pilaris, and eczematous dermatitis. However, the mechanisms underlying the dermatological abnormalities remain unclear. Here, we demonstrated that knock-in mice expressing an Hras G12S mutation (HrasG12S/+ mice) are susceptible to develop atopic dermatitis (AD)-like skin lesions, including eczema, pruritus, elevated serum IgE levels, acanthosis, and the infiltration of mast cells, basophils, and type-2 innate lymphoid cells in the dermis, after stimulation with house dust mite allergens (Dermatophagoides farinae, Dfb). Reduced skin barrier function, increased proliferation of phosphorylated ERK (p-ERK)-positive epidermal cells, and increased Th2-type cytokines as well as epithelial cell-derived cytokines, including IL-33, were observed in the skin tissue of HrasG12S/+ mice compared with Hras+/+ mice. Cultured HrasG12S/+ keratinocytes exhibited increased IL-33 expression after Dfb stimulation. PD0325901, an MEK inhibitor, ameliorated AD-like symptoms in HrasG12S/+ mice, showing decreased proliferation of p-ERK-positive epidermal cells and decreased expression of IL-33. Our findings indicate that the epidermis of HrasG12S/+ mice stimulated by Dfb strongly induced IL-33 expression and type-2 innate lymphoid cells, resulting in AD-like skin lesions. These results suggest that the epidermis of HrasG12S/+ mice are prone to development of eczematous dermatitis stimulated with house dust mite allergens.

Subject terms: Interleukins, Atopic dermatitis

Introduction

The skin is a stratified epithelium consisting of several layers of cells in various stages of differentiation. In order to maintain normal skin homeostasis, the proliferation, differentiation, and response of epidermal cells to external stimuli must be tightly regulated1. The RAS/MAPK signaling pathway plays a crucial role in cell proliferation, differentiation, and apoptosis2,3. A strong activation of the RAS/MAPK pathway in skin is known to result in epithelial cancers and melanoma4,5. Pigmented lesions, hyperkeratosis, pruritus, curly hair, and hyperplasia have also been observed in vemurafenib (a BRAF inhibitor)-treated patients6. The balance of the RAS/MAPK signaling pathway could be particularly important for epidermal homeostasis.

Noonan syndrome, Costello syndrome, and cardio-facio-cutaneous (CFC) syndrome are phenotypically overlapping genetic disorders, characterized by craniofacial dysmorphia, congenital heart defects, and psychomotor retardation7. These syndromes are commonly caused by germline mutations in components of the RAS/MAPK pathway, termed RASopathies, which constitutively activate the RAS/MAPK pathway8,9. Of these syndromes, Costello syndrome is characterized by short stature, craniofacial abnormalities, congenital heart diseases, hypertrophic cardiomyopathy, and intellectual disability10. Approximately 15% patients with Costello syndrome develop malignant tumors, including rhabdomyosarcoma and bladder carcinoma. In 2005, we identified germline HRAS mutations in patients with Costello syndrome11. A nucleotide change that cause the substitution of glycine at codon 12 to serine (p.G12S) in one allele of HRAS has been observed in 80% of Costello syndrome patients. The G12S mutation, which has been identified in somatic cancer, is an oncogenic mutation that activates the downstream pathway. Patients with Costello syndrome develop a variety of skin abnormalities, including palmoplantar keratoderma, acanthosis nigricans, eczema, loose skin (cutis laxa), darker skin color, and papillomata around nose and anus. However, the pathogenesis of dermatological abnormalities remains unclear.

We have recently generated a strain of knock-in mice expressing an Hras G12S mutation, the most frequent mutation in Costello syndrome12, which exhibited facial dysmorphia, cardiomyocyte hypertrophy, and kidney anomalies. Impaired mitochondrial fatty acid oxidation was observed in HrasG12S/+ mice fed a high-fat diet13. Skin abnormalities, including papillomas, have not been observed in young HrasG12S/+ mice (<30 weeks old) under specific pathogen-free conditions. In contrast, HrasG12S/+ mice over 30 weeks of age or high-fat diet fed-HrasG12S/+ mice had cutaneous lesions due to scratching (Supplementary Fig. 1a) under the same pathogenic-free condition. Although we have not analyzed the histology of skin of HrasG12S/+ mice over 30 weeks of age, gross appearances of the skin lesions and scratching behavior suggest that they are atopic dermatitis-like. In the current study, we tested to generate experimentally induced dermatitis in HrasG12S/+ mice and found that HrasG12S/+ mice developed more severe atopic dermatitis (AD)-like lesions than Hras+/+ mice after treatment with house dust mite allergens (Dermatophagoides farinae, Dfb). Furthermore, these AD-like skin lesions in HrasG12S/+ mice were reversed by treatment with an MEK inhibitor, PD0325901.

Results

Dfb ointment induces AD-like skin lesions in HrasG12S/+ mice

We first tested the effect of picryl chloride, which induce contact dermatitis, and imiquimod, which induce psoriasis on the skin of Hras+/+ and HrasG12S/+ mice (Supplementary Fig. 1b, c), but no difference in skin lesions was observed between them (Supplementary Fig. 1d). In contrast, the treatment with Dfb ointment developed severe dermatitis, including severe erythema, hemorrhage, scarring, and eczema, in the dorsal skin of HrasG12S/+ mice, but not in Hras+/+ mice (Fig. 1a and Supplementary Fig. 2a). The ears of HrasG12S/+ mice became thick with edema, erosion, and excoriation (Fig. 1b). The dermatitis score was significantly higher in Dfb-treated HrasG12S/+ mice than in any other group of mice (4% SDS-treated control Hras+/+ mice, Dfb-treated Hras+/+ mice, and 4% SDS-treated control HrasG12S/+ mice) on day 11 (Fig. 1c and Supplementary Table 1). Other dermatitis parameters, including the ear swelling (Fig. 1d) and the scratching behavior (Fig. 1e), increased significantly in Dfb-treated HrasG12S/+ mice compared with Dfb-treated Hras+/+ mice. Serum IgE levels were significantly higher in HrasG12S/+ mice compared to Hras+/+ in nontreated baseline (261 ± 152.2 ng/mL vs 654 ± 348 ng/mL, P = 0.039, Supplementary Fig. 3). Although the difference was not statistically significant, in 4% SDS treatment groups, IgE levels were higher in HrasG12S/+ mice compared to Hras+/+ (875 ± 596 ng/mL vs 2500 ± 2412 ng/mL, P = 0.164, Fig. 1f) as well as in the Dfb treatment groups (6930 ± 5348 ng/mL vs 14,013 ± 13,951 ng/mL, P = 0.243, Fig. 1f). These symptoms were also seen in Hras+/+ mice, but skin lesions are more severe in HrasG12/+ mice. In both groups of mice, the IgE elevations were triggered by Dfb ointment.

Fig. 1. Dfb treatment induces atopic dermatitis-like skin lesions in HrasG12S/+ mice.

Fig. 1

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a, b Skin and ear features of mice on day 28 after treatment of Dfb ointment. HrasG12S/+ mice showed dermatitis by repeated application of Dfb (a). The severity of ear swelling responses to Dfb was stronger in HrasG12S/+ than Hras+/+ mice (b). c Dermatitis scores of only 4% SDS-treated (control) Hras+/+ and HrasG12S/+, and Dfb-treated Hras+/+ and HrasG12S/+ mice (n = 8 per group). d Time course of ear thickness from Hras+/+ and HrasG12S/+ mice after treatment with 4% SDS or Dfb (n = 8 per group). e The number of scratching bouts per 30 min assessed by the video (n = 8 per group). f Serum IgE levels in Hras+/+ and HrasG12S/+ mice after treatment with 4% SDS or Dfb (n = 6 per group). Data are presented as mean ± SD. Significance in (c), (d), and (f) was analyzed by one-way ANOVA and the Tukey−Kramer method. *P < 0.05, **P < 0.001, ***P < 0.001, and ****P < 0.0001 HrasG12S/+ Dfb vs Hras+/+ Dfb; #P < 0.05, ##P < 0.01, ###P < 0.001, and ####P < 0.0001 HrasG12S/+ Dfb vs HrasG12S/+ 4% SDS; †P < 0.05, ††P < 0.01, †††P < 0.001, and ††††P < 0.0001 Hras+/+ Dfb vs Hras+/+ 4% SDS. Significance in (e) was analyzed by two-tailed Student’s t test, *P < 0.05.

We next examined if Dfb-induced dermatitis in HrasG12S/+ mice is caused by the same pathology as in Hras+/+ mice.

Histological analysis revealed hyperkeratosis and epidermal hyperplasia in the dorsal skin of Dfb-treated HrasG12S/+ mice (Fig. 2a). The epidermis of HrasG12S/+ mice became thicker than that of Hras+/+ mice, although Dfb treatment increased the epidermal thickness in both Hras+/+ and HrasG12S/+ mice (Supplementary Fig. 2b). In the AD-like skin lesions, Dfb-treated HrasG12S/+ mice displayed increased number of mast cells (toluidine blue+ and tryptase β1+), a marked increase in the numbers of T cells (CD4+) and dendritic cells (MHC class II+) (Fig. 2a, b and Supplementary Fig. 2c, e). Western blotting analysis revealed that the levels of CD4 protein were significantly increased in Dfb-treated mice compared with control mice (Supplementary Fig. 2d). In line with the acanthosis of Dfb-treated HrasG12S/+ mice, an increased number of phosphohistone H3-positive cells were observed in the suprabasal epidermis layers of HrasG12S/+ mice (Fig. 2b). Although phosphorylated ERK (p-ERK)-positive cells were also increased in the epidermis of Dfb-treated Hras+/+ and HrasG12S/+ mice, the immunostained area in HrasG12S/+ mice was significantly larger than that in Hras+/+ mice. (Fig. 2b and Supplementary Fig. 2f, g). In addition, we examined the expression of filaggrin and claudin-1 as epidermal barrier markers in AD14–16. A decreased expression of claudin-1 was observed in Dfb-treated HrasG12S/+ mice compared with control HrasG12S/+ mice (Fig. 2c, d). Together, these results indicate that Dfb-applied HrasG12S/+ mice exhibited more severe AD-like skin lesions than Hras+/+ mice, including acanthosis with hyperproliferation of p-ERK-positive cells in the epidermis, as well as increased inflammatory cells and reduced claudin-1 expression.

Fig. 2. Histological analysis reveals acanthosis with hyperproliferation of p-ERK-positive epidermal cells, increased inflammatory cells, and reduced claudin-1 expression in the dorsal skin of Dfb-treated HrasG12S/+ mice.

Fig. 2

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a Skin tissue stained with H&E and TB. b, c Immunohistochemistry of CD4, tryptase β1, p-ERK, pHH3, and claudin-1 in the skin. a–c Scale bars: 100 μm. d Lysates from the skin were immunoblotted with anti-Claudin-1 antibody. Band intensities were quantified and compared among the four groups. The expression levels were normalized to GAPDH (n = 4 in each group). Data are presented as mean ± SD. Significance was analyzed by one-way ANOVA and the Tukey−Kramer method. *P < 0.05, #P < 0.05, two-tailed Student’s t test.

The skin of Dfb-applied HrasG12S/+ mice shows an increase of itch-associated factors and inflammatory cytokines

To further characterize the AD-like skin lesions, we evaluated the levels of the itch-associated factors and inflammatory cytokines in the skin of Dfb-treated HrasG12S/+ mice. Itch-related neuronal markers, including skin Tac1, Klk7 and Klk14, mRNA levels or PAR2, and Endothelin 1 proteins17–20, were significantly higher than those in control HrasG12S/+ and Dfb-treated Hras+/+ mice (Fig. 3a–c). Regarding inflammatory cytokines, the skin mRNA levels of Il1β (proinflammatory cytokine), Il4 (Th2-related cytokine), and epidermal-derived cytokines, Il33 and thymic stromal lymphopoietin (Tslp), were significantly elevated in Dfb-treated HrasG12S/+ mice compared with control HrasG12S/+ and Dfb-treated Hras+/+ mice (Fig. 3d). IL-33 leads to the activation of type-2 innate lymphoid cells (ILC2s) through ST2, IL-33 receptor21. In Dfb-treated HrasG12S/+ mice, the skin mRNA levels of St2 were also significantly increased (Fig. 3d). Immunohistochemistry analysis revealed that Dfb-treated HrasG12S/+ mice showed enhanced expression levels of IL-33 and TSLP in the basal epidermal layers and the surface of epidermis, respectively (Fig. 3e). Likewise, the IL-33 protein levels were significantly higher in the skin of Dfb-treated HrasG12S/+ mice than in Dfb-treated Hras+/+ mice (Fig. 3f).

Fig. 3. Expression of itch-associated factors and inflammatory cytokines is enhanced in Dfb-induced skin lesions in HrasG12S/+ mice.

Fig. 3

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a, c, d Relative mRNA expression related to neuronal factors (a: Tac1 and Ngf), skin proteases (c: Klk5, Klk7, and Klk14), and inflammation (d: Il1β, Il4, Tslp, Il33, and St2) in the dorsal skin. mRNA levels were normalized to those of 18s rRNA (n = 8 per group). b, f Protein extracts from dorsal skin were immunoblotted with anti-PGP9.5, anti-PAR2, anti-Endothelin 1, and anti-IL-33 antibody (n = 4 in each group). GAPDH, same data as in Fig. 2d. f Band intensities were quantified and compared among the four groups. Expression levels were normalized to GAPDH. e Immunohistochemistry of IL-33 and TSLP. Scale bars: 100 μm. Data are presented as mean ± SD. Significance was analyzed by one-way ANOVA and the Tukey−Kramer method. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. #P < 0.05, two-tailed Student’s t test.

Increased numbers of ILC2 and increased IL-33 expression were observed in the epidermis of HrasG12S/+ mice

To investigate when and how inflammatory cytokines are induced in HrasG12S/+ mice, we performed flow cytometry on skin and ear samples 12 days after Dfb application, that is, once the AD-like skin lesions had begun to appear (Fig. 1c). The accumulation of basophils and ILC2s was observed on skin samples of Dfb-treated HrasG12S/+ mice 12 days after Dfb application (Fig. 4a). A significant increase in mast cells, eosinophils, basophils, and ILC2s was also observed in the ears of HrasG12S/+ mice (Fig. 4b). On the other hand, these immune cells were hardly detected in Hras+/+ and HrasG12S/+ mice 12 days after 4% SDS application (Supplementary Fig. 4). The mRNA levels of inflammatory cytokines in the skin from HrasG12S/+ mice 15 days after Dfb application showed that Il1β and Il33 were significantly elevated in the skin of Dfb-treated HrasG12S/+ mice compared with Dfb-treated Hras+/+ mice (Fig. 5a).

Fig. 4. An increase in ILC2s and basophils observed in the skin and ear of Dfb-treated HrasG12S/+ mice.

Fig. 4

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a, b Flow cytometric analysis of skin (a) and ear (b) cells from Hras+/+ and HrasG12S/+ mice collected 12 days after Dfb application. Eosinophils: CD45+ SSChi, siglecF+, Basophils: CD45+ siglecF− FcεRIα+ DX5+, Mast cells: CD45+ siglecF− FcεRIα+ DX5−, ILC2; CD45+ Lin− (CD3ε, CD4, CD8a, CD11c, FcεRIα, NK1.1, CD19, Ter119, CD5, F4/80, Gr-1) Sca1+ GATA3+ (n = 4 in each group). Data are presented as mean ± SD. Significance was analyzed by two-tailed Student’s t test. *P < 0.05, **P < 0.01.

Fig. 5. Expression of inflammatory cytokines is enhanced in the skin of Dfb-treated HrasG12S/+ mice in the early stage of dermatitis.

Fig. 5

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a Relative mRNA expression related to inflammation, including Il1β, Il4, Il13, Tslp, Il33, and St2 in the dorsal skin of Hras+/+ and HrasG12S/+ mice on day 15 after treatment of Dfb. mRNA levels were normalized to those of 18s rRNA (n = 6 per group). b Relative mRNA expression of Il1β, Tslp, and Il33 in Dfb-stimulated (100 ng/μl, 6 h) or vehicle-treated (PBS, 6 h) keratinocyte from Hras+/+ and HrasG12S/+ mice. mRNA levels were normalized to those of Gapdh. Results represent five independent experiments. Data are presented as mean ± SD. Significance was analyzed by one-way ANOVA and the Tukey−Kramer method. *P < 0.05, **P < 0.01, and ***P < 0.001.

Atopic dermatitis is characterized by increased serum IgE, acanthosis, loss of skin barrier function, and infiltration of immune cells, including Th2 cells, dendritic cells, eosinophils, basophils, and mast cells22,23. On that account, we next examined the response of immune cells in these mice. HRAS is known to be highly expressed in the epidermal cells, but not in immune cells24,25. Indeed, no significant difference was found in the population of immune cells from the spleen and lymph node (LN). The proliferation of naive CD4+ T cells and Th2 immune response was comparable between Hras+/+ and HrasG12S/+ mice at 7 weeks of age, suggesting that AD-like dermatitis may not be caused by different response of immune cells (Fig. 6a, b). Then we examined whether an increased production of IL-33 and TSLP was observed in primary epidermal keratinocytes in response to Dfb. Six hours after Dfb stimulation, the mRNA level of Il1β, not Tslp, was significantly elevated in cultured epidermal keratinocytes of Hras+/+ and HrasG12S/+ mice (Fig. 5b). Notably, the mRNA levels of Il33 in the Dfb-stimulated keratinocytes of HrasG12S/+ mice were significantly increased compared with those of nonstimulated HrasG12S/+ and Dfb-stimulated Hras+/+ keratinocytes, suggesting that epidermal keratinocytes with Hras G12S mutation have increased IL-33 expression after stimulation with Dfb (Fig. 5b).

Fig. 6. No significant difference was found in Th2 cytokine responses and the proliferation of naive CD4+ T cells between Hras+/+ and HrasG12S/+ mice.

Fig. 6

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a Naive CD4+ T cells were isolated from the spleen of Hras+/+ and HrasG12S/+ mice at 7 weeks of age. After that, the cells were stained with CSFE and cultured with mouse anti-CD3 monoclonal, anti-CD28 monoclonal, recombinant mouse IL-2, recombinant mouse IIL-4, and anti-IFNγ antibodies for 5 days. After incubation, the cells were fixed, permeabilized, and stained with IL-5 and IL-13 antibodies. Flow cytometric analysis was performed (n = 4 in each group). b After 3 days culture of purified naive T cells from spleen of Hras+/+ and HrasG12S/+ mice, the proliferation index was measured by flow cytometry (n = 4 in each group). Data are presented as mean ± SD. Significance was analyzed by two-tailed Student’s t test.

PD0325901 reduces skin lesions in Dfb-stimulated HrasG12S/+ mice

Treatment with MEK inhibitors ameliorates the abnormalities observed in RASopathy-related model mice26–28. We investigated the effects of an MEK inhibitor, PD0325901, on skin lesions in Dfb-treated HrasG12S/+ mice (Supplementary Fig. 5a). Ten days of treatment with PD0325901 (or saline) resulted in a significant improvement of the dermatitis score and ear swelling in HrasG12S/+ mice (Fig. 7a, b). PD0325901 treatment resulted in a marked reduction of epidermal thickness, mast cell numbers, and p-ERK-positive epidermal cells, as well as recovered expression levels of claudin-1 (Fig. 7c, d and Supplementary Fig. 5b, c). The mRNA levels of Il1β, Il4, Il33, St2, and Klk14 were significantly lower in the skin of PD0325901-treated HrasG12S/+ mice than that of vehicle (saline)-treated HrasG12S/+ mice (Fig. 7e and Supplementary Fig. 5d). These results suggest that ERK inhibition partially ameliorates Dfb-induced skin lesions in HrasG12S/+ mice.

Fig. 7. MEK inhibitor reduces the clinical severity of dermatitis in Dfb-stimulated HrasG12S/+ mice.

Fig. 7

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a, b Gross appearances, dermatitis score, and ear thickness in vehicle (saline) or PD0325901-treated Hras+/+ and HrasG12S/+ mice after 10 days of daily injections (n = 5 per group). All of the mice in these figures were treated with Dfb. The open circle and open square show vehicle group (without MEKi, with same amount of saline). c Skin tissue stained with H&E and TB in Hras+/+ and HrasG12S/+ mice with PD0325901 or vehicle treatment (n = 5 per group). d Immunohistochemistry of p-ERK and claudin-1 in skin. c, d Scale bars: 100 μm. e Relative mRNA expression of Il1β, Il4, and Il33 in dorsal skin. mRNA levels were normalized to those of 18s rRNA (vehicle group: n = 5; PD0325901 group: n = 4). Data are presented as mean ± SD. Significance was analyzed by one-way ANOVA and the Tukey−Kramer method. *P < 0.05, **P < 0.01, and ***P < 0.001 HrasG12S/+ PD0325901 vs HrasG12S/+ vehicle; ##P < 0.01, HrasG12S/+ vehicle vs Hras+/+ vehicle. †P < 0.05, one-tailed Student’s t test.

Discussion

Here, we demonstrated that mice expressing a germline Hras G12S mutation, but not Hras+/+ mice, developed AD-like skin lesions under conditions of Dfb exposure. The levels of IL-1β and epithelial cell-derived cytokines, IL-33 and TSLP, were also increased in Dfb-treated HrasG12S/+ mice. In addition, an increased production of IL-1β, IL-4, and IL-33, as well as inflammatory cells, basophils, and ILC2s, was observed in the dorsal skin of HrasG12S/+ mice before the development of AD-like skin lesions. Analysis of the underlying mechanism revealed that Dfb-stimulated keratinocytes in HrasG12S/+ mice induced IL-33 production, while, in naive CD4+ T cells from the spleen, the Th2 immune response was comparable between Hras+/+ and HrasG12S/+ mice. Finally, the inhibition of ERK activation by PD0325901 treatment ameliorated the AD-like skin lesions and IL-33 production. Together, these data indicate that germline Hras G12S activating mutation causes AD-like skin lesions via the ERK/IL-33 axis (Fig. 8).

Fig. 8. Germline HrasG12S mutation causes AD-like skin lesions via ERK/IL-33 axis. Exposure to Dfb allergen induces AD-like skin lesions, including eczema, acanthosis, and pruritus in HrasG12S/+ mice.

Fig. 8

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Dfb-treated HrasG12S/+ keratinocytes show increased IL-33 expression through hyperproliferation of p-ERK-positive epidermal cells. Excess IL-33 activates basophil- and ILC2-containing ST2 receptors. These activated immune cells induce the production of type-2 inflammatory cytokines, such as IL-4. Furthermore, excess IL-33 can activate ERK signaling, resulting in reduced claudin-1 expression and skin barrier dysfunction. DC dendritic cells.

In the present study, after repeated stimulation with Dfb, HrasG12S/+ mice showed hyperproliferation of p-ERK-positive epidermal cells and increased IL-33 expression in the dorsal skin. Increased IL-33 expression was also observed in primary epidermal keratinocytes from HrasG12S/+ mice after Dfb stimulation, which is similar to the reports that the activation of RAS/MAPK signaling was associated with increased IL-33 expression in cancer cells29,30. Recently, IL-33 was found to induce the Th2 inflammatory response in allergic diseases, especially AD31. Excess IL-33 is also associated with skin barrier dysfunction and ILC2 functions, which is partially regulated by the RAS/MAPK signaling pathway32,33. Epithelial-specific IL-33 transgenic mice have been found to develop AD-like dermatitis, including acanthosis, pruritus, increased IgE serum levels, reduced claudin-1 expression, and increased production of eosinophils, mast cells, and ILCs33,34. Of note, IL-33 and its receptor, ST-2, are highly expressed in the skin-derived ILC2s of AD patients and lung ILC2s of patients with allergic airway diseases, respectively35,36. Importantly, consistent with the phenotype of IL-33 transgenic mice and AD patients, Dfb-treated HrasG12S/+ mice showed AD-like skin lesions, reduced claudin-1 expression, increased IL-33 expression, hyperproliferation of p-ERK-positive epidermal cells, and increased ILC2 production. Collectively, excess IL-33 could lead to ERK activation, resulting in an increase of ILC2s and impaired skin barrier (Fig. 8).

The pathogenesis of skin lesions observed in Costello syndrome includes dermal connective tissue abnormalities (cutis laxa and deep palmer and plantar creases), hyperproliferative skin disease (palmoplantar keratoderma, cutaneous papilloma, and acanthosis nigricans), and inflammatory skin abnormalities (sensitive skin, eczema, and pruritus). A previous study showed that, in the skin fibroblasts of Costello syndrome, elastic fibers were not assembled due to a functional deficiency of the elastin-binding protein as a result of an unusual accumulation of chondroitin sulfate-bearing proteoglycans37,38. Regarding the hyperproliferative skin lesions, it has been reported that the root cause of papillomas, hyperkeratosis, and epidermal hyperplasia, such as psoriasis, is the activation of the RAS-MAPK pathway39–42. However, the pathophysiological mechanism of inflammatory skin abnormalities in Costello syndrome remains unclear. In the present study, Dfb-treated HrasG12S/+ mice displayed pruritus and eczema. Recently, mice with epidermis-specific BRAF/RAF1 deficient also showed AD-like dermatitis, which is characterized by increased serum IgE levels and a Th2 response43. The elevated IgE levels have not been systematically examined or reported in Costello syndrome patients. However, it is possible that the allergic reaction stimulated by house dust mites could be involved in the development of inflammatory skin abnormalities in Costello syndrome patients, including sensitive skin, pruritus, and eczema.

At present, acitretin has been reported to treat palmoplantar keratosis in patients with Costello and CFC syndrome44,45. Several reports have demonstrated that MEK inhibitors improve HRAS-driven tumorigenesis46, impaired enamel formation in the teeth27, and long-term depression in the hippocampus in Hras G12V knock-in mice28, as well as hyperkeratosis and hyperplasia in the forestomach of BrafQ241R/+ mice26. In the present study, PD0325901 treatment of the AD-like skin lesions in HrasG12S/+ mice was found to reverse these lesions by reducing hyperproliferation of p-ERK-positive epidermal cells and the production of inflammatory cells and cytokines, including IL-1β, IL-4, and IL-33. Treatment with U0126, an MEK inhibitor, in human keratinocytes, has also been found to restore the reduced expression levels of claudin-1 and filaggrin, and increase ERK activation through excess IL-3333,47. Indeed, reduced claudin-1 expression was improved in Dfb-treated HrasG12S/+ mice after PD0325901 treatment. Recently, hypertrophic cardiomyopathy in Noonan syndrome patients were treated by MEK inhibitor48. So, MEK inhibitors could be effective in patients with RASopathies. The most common side effect of trametinib (MEK inhibitor) in human patients is skin rush, and common toxicity associated with vemurafenib (BRAF inhibitor) is cutaneous abnormalities such as keratoacanthoma and squamous cell carcinoma by the mechanism of paradoxical MAPK pathway activation49. Therefore, the balance of RAS/MAPK signaling plays an important role in the emersion of skin abnormalities. Adjusting dosage of MEK inhibitors may be effective on skin lesions of patients with Costello syndrome.

Here, Dfb-treated HrasG12S/+ mice exhibited increased IL-33 expression through hyperproliferation of p-ERK-positive epidermal cells. Additionally, we show that PD0325901 treatment ameliorated the AD-like skin lesions in HrasG12S/+ mice under conditions of exposure to Dfb. Thus, it will be interesting to investigate whether treatment with IL-33 antibody reduces the AD-like skin lesions in HrasG12S/+ mice. Our findings provide additional perspective that HrasG12S/+ mice will serve as a valuable model to study pathophysiology and potential therapeutic approaches in AD.

Materials and methods

Mice

HrasG12S/+ mice on a C57BL/6J background have been described previously13. Male mice were analyzed in this study.

Genotyping

The genomic DNA was extracted from the tail tissue using a Maxwell 16 Mouse Tail DNA Purification Kit (Promega, Madison, WI, USA) or the alkaline lysis method. For the alkaline lysis method, a small piece of each tail (2 mm) was incubated in 50 mM NaOH for 20 min at 95 °C. After the addition of 1 M Tris-HCl (pH 8.0), the extracts were used for PCR. Genotyping of Hras+/+ and HrasG12S/+ mice was performed by PCR using KOD FX Neo (TOYOBO, Osaka, Japan). The primers used for PCR have been described previously13,50.

Induction of dermatitis

Atopic dermatitis-like skin lesions were induced in male mice at 9 weeks of age, according to the manufacturer’s instructions. The mice were anesthetized with isoflurane and their dorsal hair was removed using an electric clipper (2000AD; Natsume Seisakusho, Tokyo, Japan). For sensitization, 100 mg of Biostir AD (Biostir Inc, Kobe, Japan), an ointment of Dfb extract, was applied to the shaved dorsal skin and ears. Three or four days later, hair growth was removed with an electric clipper, and 150 μl of 4% SDS (Sigma-Aldrich, St. Louis, MO, USA) was applied to the shaved dorsal skin and ears to disrupt the skin barrier. After 2 h, 100 mg of Biostir AD was applied to their shaved dorsal skin and ears to induce AD-like skin lesions. These procedures were repeated twice a week for 25 days. The mice were sacrificed on day 32 to collect skin and ear samples (Supplementary Fig. 2a). To assess the effect of Dfb ointment to Hras+/+ and HrasG12S/+ mice, they were randomly divided into four groups (4% SDS-treated Hras+/+, 4% SDS-treated HrasG12S/+, Dfb-treated Hras+/+, and Dfb-treated HrasG12S/+) using single blinding test.

Evaluation of dermatitis and ear thickness

The dermatitis scores were evaluated twice a week according to the development of four symptoms: erythema/hemorrhage of dorsal skin, scarring/dryness of dorsal skin, edema of ear, and excoriation/erosion of ear51. The total dermatitis score (maximum score: 12) was defined as the sum of individual scores (none: 0, mild: 1, moderate: 2, severe: 3) for each symptom (Supplementary Table 1). Ear thickness was measured with a digimatic micrometer (CLM1-15QM; Mitutoyo, Kanagawa, Japan).

Measurement of scratching behavior

On day 28, scratching behavior was monitored by video (GZ-HM890; JVC, Kanagawa, Japan) for 30 min. The number of scratching bouts was assessed by replaying the video. Each incidence of scratching behavior was defined as rubbing of ears and dorsal skin with forepaws, hind paws, and mouth.

Histology and immunohistochemistry

The dorsal skins were fixed in 10% neutral buffered formalin for paraffin-embedded specimen and 4% paraformaldehyde for frozen specimen. Embedded tissues were sectioned at 3 μm (paraffin-embedded specimens) or 8 μm (frozen specimens). Paraffin-embedded specimens were stained with hematoxylin and eosin (H&E) and toluidine blue (TB). Epidermal thickness was measured in five randomly selected areas (900 × 700 μm) of each H&E-stained sample. Mast cells were counted in ten randomly selected areas (450 × 350 μm) of each TB-stained sample. For immunohistochemistry, the paraffin-embedded sections were deparaffinized using xylene and rehydrated with ethanol. Frozen specimens were dried sufficiently with a dryer. Antigens were activated using a Histofine simple stain kit (Nichirei Bio Sciences, Tokyo, Japan). The antibodies used are described in Supplementary Table 2. Signals were visualized with a DAB Substrate Kit (Nichirei Bio Sciences). Sections were counterstained with hematoxylin. p-ERK immunostained area/epidermis (%) was measured in five randomly selected areas (900 × 700 μm) of each p-ERK-stained sample.

Quantitative reverse transcription-PCR

Total RNA extraction and purification of keratinocytes was performed according to the standard procedures using an RNeasy Mini Kit (Qiagen, Hilden, Germany) and QIAshredder (Qiagen). The extraction and purification of the total RNA from the dorsal skin and cDNA synthesis were performed as previously described26. Quantitative real-time PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO) in a StepOnePlus (Thermo Fisher Scientific, Waltham, MA, USA). The amplification primers are described in Supplementary Table 3. Each sample was run in duplicate.

Western blotting

Skin tissue was homogenized in lysis buffer (10 mM Tris-HCl pH 7.5 and 1% SDS) containing phosphatase and protease inhibitor (P5726 and P8340; Sigma-Aldrich). Supernatants were collected after centrifugation and the protein concentration was determined using a Bio-Rad Protein Assay (Bio-Rad Laboratories, Hercules, CA). Western blot analyses were performed as previously described26. Briefly, the proteins were transferred onto nitrocellulose membranes and blocked with 5% non-fat milk in Tris-buffered saline with Tween-20 (10 mmol/L Tris-HCl, pH 8.0, 150 mmol/L NaCl, and 0.1% Tween-20) for 1 h at room temperature. The membranes were incubated overnight at 4 °C with the antibodies described in Supplementary Table 2. All membranes were visualized using the Western Lightning ECL Plus Kit (PerkinElmer, Waltham, MA, USA). The band intensities were quantified using NIH ImageJ software.

ELISA

Serum IgE was determined using LBIS Mouse IgE ELISA Kit (FUJIFILM Wako Shibayagi, Gunma, Japan), according to the manufacturers’ instructions.

Skin and ear immune cell preparation and flow cytometry

The hair on the dorsal skin was shaved off before removing the subcutaneous fat and connective tissue. The skin and ear were cut into pieces and incubated in RPMI 1640 (FUJIFILM Wako, Osaka, Japan) containing 10% fetal calf serum (FCS), Collagenase (C5138; Sigma-Aldrich), Dispase (17105-04; Gibco BRL, Palo Alto, CA), DNase I, and Hyaluronidase (Sigma-Aldrich) for 90 min to isolate single-cell suspension52. After incubation in 2.4G2 (130-092-575; Miltenyi Biotec, Auburn, CA) as an Fc-blocking reagent, cells were stained with the antibodies described in Supplementary Table 2. Flow cytometry data were acquired by Spectral Analyzer (SP6800; SONY, Tokyo, Japan) and analyzed with FlowJo (Becton, Dickinson and Company, USA).

Spleen and LN immune cell preparation and flow cytometry

The spleen and inguinal LN from mice were cut into pieces and incubated in ACK lysis buffer (150 mM NH4Cl, 10 mM KHCO3, 0.1 mM ethylenediaminetetraacetic acid 2Na, pH 7.2) for 2 min. After incubation in 2.4G2 (Miltenyi Biotec) as an Fc-blocking reagent, the cells were stained with the antibodies described in Supplementary Table 2. The flow cytometry data were acquired by BD FACSCANTO II (BD Bioscience, Franklin Lakes, New Jersey) and analyzed with FlowJo (Becton).

Spleen immune cell preparation and naive CD4+ T-cell sorting assays

Mouse naive CD4+ T cells were isolated from the spleen by magnetic sorting according to standard procedures using a naive CD4+ T-cell isolation kit (Miltenyi Biotec). CFSE (C1157; Invitrogen, San Diego, CA)-stained cells were suspended in RPMI 1640 medium containing 10% FCS. The cell suspensions were cultured and stimulated with plate-bound mouse anti-CD3 and anti-CD28 monoclonal antibodies, recombinant mouse IL-2, IL-4, and anti-IFNγ antibodies for 5 days in a 37 °C, 5% CO2 incubator. The cell suspensions were re-stimulated with phorbol 12-myristate 13-acetate (Sigma-Aldrich), ionomycin (Sigma-Aldrich), and GolgiPlug (BD Bioscience) for 3 h. Cells were fixed and permeabilized with fixation/permeabilization solution (BD Bioscience) and stained with IL-5 and IL-13 antibodies. After 3 days of culturing the purified naive T cells, the proliferation index was determined by flow cytometry. The flow cytometry data were acquired by Spectral Analyzer (SONY) and analyzed with FlowJo (Becton).

Cell culture and stimulation

Primary epidermal keratinocytes were isolated from newborn mice (0- or 1-day-old) and cultured according to recommended protocol using a CnT-PR (CELLnTEC, Bern, Switzerland), CnT-GAB10 (CELLnTEC), Dispase II (Gibco BRL), and TrypLE Express Enzyme (Gibco BRL). Briefly, the skin was peeled from the neonates and incubated in CnT-PR with CnT-GAB10 (10 μg/mL gentamycin and 0.5 μg/mL amphotericin B) and 12.5 U/mL dispase for 16 h at 4 °C. The epidermis was separated from the dermis and incubated in TrypLE Express Enzyme for 20–30 min at room temperature. The cells were separated from the epidermal sheet, seeded at a density of 4 × 104 cells/cm2 in CnT-PR medium, and cultured in a 37 °C, 5% CO2 incubator. Medium change was performed after 24 h and 4 days after seeding. After 5 days of culture, 100 ng/mL of mite dermatophagoides farina crude extract (LSL, Tokyo, Japan) in phosphate buffered saline (PBS) was added to the culture medium. After 6 h of incubation, the cells were collected for quantitative reverse transcription-PCR analysis.

Mouse treatment

PD0325901 (Sigma-Aldrich) was dissolved in ethanol at a concentration of 5 mg/mL and prepared in saline at a concentration of 0.125 mg/mL. PD0325901 (1.0 mg/kg/body weight) was intraperitoneal injected into anesthetized mice daily from days 12 to 21 after the start of Dfb application (Supplementary Fig. 5a).

Statistical analysis

All data are presented as mean ± standard deviation (SD). Statistical analyses were performed using Student’s t test for comparisons between two groups. Comparisons among four groups were performed using one-way ANOVA and the Tukey−Kramer method. All data were analyzed using JMP Pro 14 software (SAS, Cary, NC). P value below 0.05 was considered statistically significant.

Study approval

All animal studies were approved by the Animal Care and Use Committees of Tohoku University.

Supplementary information

Supplementary figure legend (33.7KB, docx)
Supplementary table (40.4KB, docx)
Supplementary Figure 1 (1MB, png)
Supplementary Figure 2 (1.3MB, png)
Supplementary Figure 3 (203.3KB, png)
Supplementary Figure 4 (895.4KB, png)
Supplementary Figure 5 (391.6KB, png)

Acknowledgements

We wish to thank Riyo Takahashi, Kumi Kato, and Yoko Tateda for technical assistance. We would also like to acknowledge the support of the Biomedical Research Core of the Tohoku University Graduate School of Medicine. This work was supported by Japan Agency for Medical Research and Development under Grant to Y. A. (JP18ek0109241), by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 17H04223 to Y. A., and by JSPS KAKENHI Grant Number 18K07811 to S.-i.I.

Data availability

The authors declare that all other data of this study are available from the corresponding author upon reasonable request.

Conflict of interest

The authors declare that they have no conflict of interest.

Footnotes

Edited by E. Candi

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Information accompanies this paper at (10.1038/s41419-020-02845-8).

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary figure legend (33.7KB, docx)
Supplementary table (40.4KB, docx)
Supplementary Figure 1 (1MB, png)
Supplementary Figure 2 (1.3MB, png)
Supplementary Figure 3 (203.3KB, png)
Supplementary Figure 4 (895.4KB, png)
Supplementary Figure 5 (391.6KB, png)

Data Availability Statement

The authors declare that all other data of this study are available from the corresponding author upon reasonable request.

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