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. 2020 Aug 5;107(3):509–521.e7. doi: 10.1016/j.neuron.2020.05.013
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Tamoxifen Sigma-Aldrich Cat # T5648-1G
Corn Oil Sigma-Aldrich Cat # C8267-500 ml
NaCl VWR (Merck) Cat # 1.06404.1000
Sucrose Sigma-Aldrich Cat # 16104
NaHCO3 VWR (Merck) Cat # 1.06329.1000
D-glucose VWR (Merck) Cat # 1.08342.1000
KCl VWR (Merck) Cat # 26764.232
Na2HPO4 VWR (Merck) Cat#1.06580.0500
NaH2PO4 VWR (Merck) Cat # 1.06346.0500
CaCl2 VWR (Merck) Cat # 1.02382.0250
MgCl2 Honeywell Cat # M9272-1KG
HEPES Sigma-Aldrich Cat # M3375-100G
EGTA Sigma-Aldrich Cat # EO396-100G
Na2ATP Sigma-Aldrich Cat # A3377-100G
Polyvinylpyrrolidone (PVP) Sigma-Aldrich Cat # PVP10-100G
Sodium azide Sigma-Aldrich Cat # S8032-25G
Osmium tetroxide Science services (EMS) Cat # 19130
Durcupan ACM Resin Single component A Sigma-Aldrich Cat # 44611-100 ml
Durcupan ACM Resin Single component B Sigma-Aldrich Cat # 44612-100 ml
Durcupan ACM Resin Single component C Sigma-Aldrich Cat # 44613-100 ml
Durcupan ACM Resin Single component D Sigma-Aldrich Cat # 44614-100 ml
Uranyl acetate 2 H2O Serva Cat # 77870.02
Lead (II) nitrate Sigma-Aldrich Cat # 228621-100G
Acetone, HPLC grade ChemLab Cat # CL00.0172.2500
Ketamine Intervet Z.Nr. 8-00335 100 mg/ml
Xylazine Graeub Z.Nr. 8-00178 20 mg/ml
Lidocaine Sigma L-1026-1VL
Dexpanthenol ointment Bayer Cat # PZN 0829388
Cyanoacrylate superglue Uhu Cat #45570 3 g
Metacam Boehringer 2 mg/ml
Silicone elastomer Kwik-cast, World Precision Instruments N/A
Potassium D-Gluconate Sigma-Aldrich Cat # G4500-100 g
NaGTP Sigma-Aldrich Cat # G8877-250 mg
Biocytin Molecular probes Cat # B1592
Paraformaldehyde TAAB Cat # FO 17/1
Glutaraldehyde Carl Roth Cat # 4157.1
Saturated picric acid solution Sigma-Aldrich Cat # P6744-1GA
Triton X-100 Sigma-Aldrich Cat # X100-100ml
Avidin-biotinylated horseradish peroxidase complex ABC, Vector Laboratories Cat # PK6100
3,3′-Diaminobenzidine tetrahydrochloride Sigma-Aldrich Cat # D5637-5 g
Cobalt Sigma-Aldrich Cat # C8661-25 g
Nickel (II) chloride hexahydrate Sigma-Aldrich Cat # 223387-25 g
Mowiol 4-88 Carl Roth Cat # 713.2
Cytochalasin D Tocris Cat # 1233
Latrunculin B Tocris Cat # 3974/1
H-89 dihydrochloride Tocris Cat # 2910/10
Tetrodotoxin citrate Tocris Cat # 1069/1
Tetraethylammonium chloride Sigma-Aldrich Cat # T2265
Cesium chloride Sigma-Aldrich Cat # C3011-25 g
H2O2 Sigma-Aldrich Cat # 95321-100 ml
Tris(hydroxymethyl)aminomethane Sigma-Aldrich Cat #252859-100 g
Glycerol Sigma-Aldrich Cat # G9012-500 ml
HCl Merck 1.09057.1000
Experimental Models: Organisms/Strains
Prox1-CreERT2 mouse line Bazigou et al., 2011 PMID: 21765212
C57BL6/J wild-type mice Charles River Germany (from The Jackson Laboratory) RRID:IMSR_JAX:000664
B6;129S-Gt(ROSA)26Sortm32(CAG-COPA∗H134R/EYFP)Hze/J mouse line The Jackson Laboratory RRID:IMSR_JAX:012569
Wistar Rats Preclinical facility, IST Austria
Janvier Labs		 RRID:RGD_13508588
Software and Algorithms
Multiclamp (version 1.3.0.05) Axon Instruments/Molecular Devices https://www.moleculardevices.com/
Igor Pro (version 6.3.4.1) WaveMetrics https://www.wavemetrics.com/
Radius Software EMSIS https://www.emsis.eu/products/software/radius/
Stimfit (version 0.14.14) Guzman et al., 2014 PMID: 24600389
AxoGraph (version 1.7.2) AxoGraph https://axograph.com/
Fiji (ImageJ version 2.0.0-rc-69/1.52n) NIH, Open source https://fiji.sc/
Graphpad Prism (version 8) Graphpad https://www.graphpad.com/
Adobe Illustrator (version 23.0.4) Adobe https://www.adobe.com/products/illustrator.html
HEKA Patchmaster acquisition software (2x90.1) HEKA https://www.heka.com/
Coreldraw X8 Coreldraw https://www.coreldraw.com/en/
MATLAB 2016, 2018 MathWorks https://www.mathworks.com/
Coreldraw 2018 Coreldraw https://www.coreldraw.com/en
Origin 2018 OriginLab https://www.originlab.com/
Python 2.7 Python https://www.python.org/
Mathematica 12.0.0.0 Wolfram Research https://www.wolfram.com/mathematica/
Other
Borosilicate glass (2 mm outside/ 1 mm inside) Hilgenberg Cat# 1907542