FIGURE 1.

Identification of the fragments of radiolabeled 15S rRNA coprecipitating with Dmr1p. (A) Slot-blot macro array hybridization of the RNA fragments from the unbound and bound fraction with 27 80-nt oligonucleotide probes (numbers to the left of respective slots, probe #1 corresponds to the 3′ end of the molecule, and probe #27 to the 5′ end) covering the entire length of the 15S rRNA sequence. N is an unrelated negative control probe. A representative result of three replicates is shown. Details of the procedure are described in the text. (B) Ratios of bound to unbound signal for each probe. Values from three replicates with Dmr1p-MBP-His₆ fusion protein are shown as black circles. Open circles represent results obtained using the MBP-His₆ tag as negative control. (C) Sequences of 15S rRNA fragments complementary to probe #15 and probe #20 showing the highest bound to unbound ratio. AUA trinucleotide repeats unique to these two fragments are underlined.