Figure 4.

Effect of Vau-1/Vau-2 extracts or arbutin on proportion of neutrophils at various maturation states. (A) Gating strategy for neutrophils. The first panel shows the histogram for Ly6G expression and gating of Ly6G⁺ positive cells. The second panel shows the dot-plot histogram with scales for Ly6G⁺ cells vs. SSC-A (the size surface volume), that determines the shape and granularity of the BM-derived neutrophils. The following populations were defined on the basis of SSC-A and Ly6G positivity (staining with fluorescein isothiocyanate (FITC)-labelled antibody against Ly6G). Upper Left (P1 gate)—SSC^hiLy6G^low immature transient cells; Upper right (P2 gate)—SSC^hiLy6G^hi mature cells; Lower left (P3 gate)—SSC^lowLy6G^low immature cells; Lower right (P4 gate)—SSC^lowLy6G^hi mature transient cells; (B) Proportion of neutrophils at various maturation states in BM cells incubated in the presence or absence of Vau-1, Vau-2 or arbutin for 36 h. Data represent mean ± SD of sample from 2 experiments with neutrophils isolated and pooled from 7 mice and assayed in duplicate.