Hepatic Antifibrotic Pharmacotherapy: Are We Approaching Success?

Abstract

The incidence rate and mortality of liver fibrosis caused by various etiologies are high throughout the world. Liver fibrosis, the subsequent cirrhosis and other serious related complications threaten the health of patients and represent a serious medical burden; yet, there is still a lack of approved methods to prevent or reverse liver fibrosis. Therefore, effective hepatic antifibrotic drugs are urgently needed. The activation and proliferation of hepatic stellate cells are still the mechanisms of fibrosis that remain the focus of therapeutic research. In recent years, significant progress has been made in the development and applicability of antifibrosis drugs. In this review, we summarize the effectiveness and safety of available antifibrosis drugs utilizing different targets. In addition, some characteristics of antifibrosis drugs in phase II and III trials are introduced in detail.

Introduction

Liver cells usually regenerate after injury, but when injury and inflammation persist, the liver cannot regenerate normally and fibrosis will occur. Liver fibrosis is a pathological outcome of the repair response to chronic liver injury caused by any etiology, such as hepatitis B or C virus infection (HBV/HCV), nonalcoholic fatty liver disease (NAFLD), alcoholic steatohepatitis, autoimmune hepatitis, or cholestatic liver disease. Tissue remodeling and repair can lead to the production and deposition of a large number of collagens, fibronectin, undulin, laminin, and other extracellular matrixes (ECMs) and eventually to the formation of scar tissue.1 Long-term liver fibrosis will promote the accumulation of a fibrous matrix and destroy the normal function and structure of the liver. If left untreated, it will eventually progress to liver cirrhosis or carcinoma, which are the major causes of death due to chronic liver disease. Therefore, there is a dire need for an antifibrotic drug that can not only inhibit the progression of hepatic fibrosis but also reverse its progression.

However, to date, there is no effective chemical drug in the clinic for the treatment of liver fibrosis. Therefore, research on hepatic antifibrotic drugs is a ‘hot topic’. At present, the main drug treatment strategies for fibrosis include the treatment of primary diseases, control of the inflammation, regulation of ECM synthesis and degradation, improvement in liver parenchyma cell injury, and apoptosis. Although there are no approved pharmacotherapies for fibrosis, sustained effort and remarkable progress have been made in the research on antifibrosis drugs in recent years, particularly for drugs for NAFLD-related fibrosis. The present review will emphasize the progress that has been made in efficacy and safety of potential drugs for the treatment of fibrosis and highlight underlying challenges in the future.

Activated hepatic stellate cells (HSCs) are still the primary effector cell of fibrosis

Myofibroblasts (MFs) are the main cells that produce ECM (e.g., collagens) in the process of chronic liver cell damage. MFs do not exist in normal liver tissue. The major source of MFs is HSCs, although a small part of MFs comes from portal vein fibroblasts,2 hematopoietic stem cell fibroblasts, and bone marrow-derived fibrocytes.3 Interestingly, in the model of cholestatic liver injury, portal vein fibroblasts are the major source of MFs at the onset of injury, but HSCs are still the main source of MFs in the later stages.4 Nevertheless, it is controversial whether MFs originate from hepatocytes or cholangiocytes by the epithelial-to-mesenchymal transition or endothelial mesenchymal transition.5

In the healthy liver, HSCs show a quiescent phenotype. HSCs are located in the space of Disse, accounting for 5-8% of the total cells of the liver.6 There is much evidence that the activation of HSCs plays a critical role in fibrosis. Transforming growth factor (TGF)-β, osteopontin, and platelet-derived growth factor (PDGF) are the most important cytokines that promote the activation of HSCs and the proliferation of ECM. Many other cytokines and intracellular signal transduction pathways are also involved in the activation of HSCs. Therefore, drugs targeting the activation of HSCs will become a therapeutic strategy for hepatic antifibrosis.

Reducing the number of activated HSCs is essential for reversing and treating liver fibrosis. The three main pathways that can help eliminate activated HSCs are the return to quiescent phenotype, apoptosis, and senescence (Fig. 1).7^–9 At present, there is solid evidence that the reversal of HSC activation to the quiescent cell state plays a dominant role.10 Thus, promoting the apoptosis of HSCs may be a potential antifibrotic target. In addition, multiple other cell types and factors play important roles in the process of liver fibrosis, such as immune cells, particularly macrophages,11 liver progenitor cells, autophagy,12 and epigenetics.13^,14 Pathways and signals derived from intrahepatic or extrahepatic events also provide some potential targets for the drug treatment of liver fibrosis.

Fig. 1. Pathogenesis of liver fibrosis.

The schematic summarizes the fate of hepatic stellate cells and their role in liver fibrosis.Abbreviations: HSC, hepatic stellate cell; ECM, extracellular matrix.

Pharmacological therapy strategies for liver fibrosis

Currently, with a better understanding of the pathogenesis of fibrosis, an increasing number of potential drugs that reverse fibrosis are in phase II or III trials. Here, we briefly review the current status of promising antifibrotic drugs in clinical trials (Table 1). The following represent the latest advances in pharmacological therapy strategies for antifibrosis and are outlined in Fig. 2.

Table 1. Promising pharmacological agents for hepatic antifibrosis in clinical trials.

Mechanism of antihepatic fibrosis	Drug	Pharmaceutical company	Indication disease	Current phase	Trial name	Participants	Patient	Major AEs
FXR agonist	OCA	Intercept	NASH	III	REGENERATE	Fibrosis	2370	Pruritus; Drug toxicity
					REVERSE	Compensated cirrhosis	540
CCR2/CCR5 inhibitor	Cenriviroc	Allergan	NASH	III	AURORA	Fibrosis	2000	Fatigue; Diarrhea
					CENTAUR	Fibrosis (S 1-3)	289
ASK-1 inhibitor	Selonsertib	Gilead	NASH	III	STELLAR- 3	Advanced fibrosis	800	Nausea, Headache, Nasopharyngitis, Upper abdominal pain, Sinusitis, Back pain, Fatigue
					STELLAR- 4	Compensated cirrhosis	883
Pyridinone derivative	Pirfenidone	Shionogi	HCV	II	-	Chronic hepatitis C	34	Gastrointestinal, Skin-related AEs
FGF‑21 analog	Pegbelfermin	Bristol Myers Squibb	NASH	IIa	-	Fibrosis stage 1-3	75	Diarrhea, Nausea
FGF19 analog	Aldafermin (NGM282)	NGM Biopharmaceuticals	NASH	IIb	-	Fibrosis (S 1-3)	82	Injection site reactions, Gastrointestinal symptoms
			PSC	II	-	Fibrosis	62
PPAR α/δ agonist	Elafibranor	Genfit	NASH	III	RESOLVE-IT	F2-3	2000	Renal impairment/failure
			PBC	II	-		45
siRNA against HSP47	BMS-986263	Bristol Myers Squibb	HCV	II	-	METAVIR Stage 3- 4	62	Mild to moderate infusion related reactions

Abbreviations: AEs, adverse events; ASK1, apoptosis signal-regulating kinase 1; CCR2/5, C-C chemokine receptor type 2; FGF, fibroblast growth factor; FXR, farnesoid X receptor; HSP, heat shock protein; NASH, non-alcoholic fatty liver disease; OCA, obeticholic acid; PBC, primary biliary cholangitis; PPAR, peroxisome proliferator-activated receptor; PSC, primary sclerosing cholangitis; siRNA, small interfering RNA.

Fig. 2. Pharmacological therapy strategies for hepatic antifibrosis.

Abbreviations: ACE, angiotensin converting enzyme inhibitors; CB1, cannabinoid receptor 1; CCR, CC chemokine receptor; DAA, direct-acting antiviral agents; ECM, extracellular matrix; FGF, fibroblast growth factor; FXR, farnesoid X receptor; HSC, hepatic stellate cell; NA, nucleos(t)ide analog; NK, natural killer; PPARs, peroxisome proliferator-activated receptor; TGF, transforming growth factor; LOXL2, lysyl oxidase-like protein 2; TIMP, tissue inhibitor of metalloproteinase.

Curing or controlling the primary disease

There is no doubt that the control or cure of primary liver disease is an efficient and effective way to reverse the progression of fibrosis. Many studies have proven that if the underlying etiology is effectively controlled or eliminated, liver fibrosis can be reversed, the structure and function of the liver can be restored to normal, and the risk of developing cirrhosis and tumors can be decreased.15

The most complete clinical evidence comes from chronic viral hepatitis. Clearance of HCV or long-term effective inhibition of HBV with potent nucleos(t)ide analogs (NAs) can effectively reduce and even reverse the progression of fibrosis and cirrhosis.16^,17 It is worth noting that if there has been liver cirrhosis with significant portal hypertension, even after virologic cure, there may still be signs of clinical disease progression in a short period of time, including recurrent complications. In nonalcoholic steatohepatitis (NASH), a loss of up to 10% of total body weight can improve the fibrosis stage.18 It was observed that, despite sustained virologic response, 8-12% of patients with HCV still showed progress in the degree of fibrosis or cirrhosis17^,19 and still retained a 5% risk of liver cancer.20 The possible cause of fibrosis progression or liver primary cancer is that antiviral therapy starts too late and is more likely to be associated with other underlying liver diseases, most of which are NAFLDs.

In recent years, liver fibrosis and cirrhosis caused by NAFLD have attracted increasing attention, as these will become the major etiologies of liver transplantation or hepatocellular carcinoma in the near future.21 Insulin resistance, oxidative stress, and metabolic disorders are the main pathological bases for the occurrence of NAFLD and the progression of fibrosis. Many therapeutic strategies and new research drugs for NAFLD fibrosis mainly target reducing insulin resistance or abnormal metabolism to reduce the production of free fatty acids, lipotoxicity, excessive accumulation of triglycerides in hepatocytes, mitochondrial dysfunction, and endoplasmic reticulum stress.22 Many agonists of receptors for the NAFLD metabolic pathway have been found to be effective in inhibiting fibrosis, such as farnesoid X receptor (FXR) antagonist, peroxisome proliferator-activated receptors (PPARs), and glucagon-like peptide-1 (GLP-1). On the one hand, FXR plays a central role in glucose and lipid metabolism. On the other hand, FXR can also down-regulate the adipogenesis inducer SREPB-1c to induce fibroblast growth factor (FGF) 19 and reduce the production of endogenous bile acids.23 Obeticholic acid (OCA), a strong FXR agonist, has been demonstrated to improve biomarkers of inflammation and reduce the degree of fibrosis stage in patients with type 2 diabetes and NAFLD.24^,25 Of course, other nonsteroidal FXR ligands, including AKN-083 (Allergan, Dublin, Ireland), troifexor (Novartis, Basel, Switzerland), LMB763 (Novartis), and GS-9674 (Gilead Sciences, Foster City, CA, USA), are also in vigorous experiments and studies, and are expected to become prospects for antifibrosis drugs. In addition to the FXR agonist, PPARs (PPARα, PPARβ/δ, and PPARγ) have been widely tested in NAFLD. Although pioglitazone (PPARγ agonist) has been found to reduce liver fibrosis in patients with NASH without type 2 diabetes,26 the limitations of patients with heart failure and drug-related weight gain may limit its clinical application for liver fibrosis.

As reviewed above, PPARs can not only improve lipid metabolism and insulin sensitivity but can also reduce liver inflammation. In the phase II trial of 276 patients with NASH without fibrosis treated with elafibranor (PPAR α/δ agonist) for 1 year, the degree of fibrosis did not progress after receiving elafibranor (120 mg/d) versus the placebo group (19% vs. 12%; p=0.045).27 Although the reported elafibranor was well tolerated, renal impairment (increase in serum creatinine, p<0.001) or renal failure needs to be vigilantly monitored and further observed. The phase III trial of elafibranor for patients with NASH with fibrosis (F2- F3) is ongoing. We look forward to the further results of effectiveness and safety. In addition, a phase II trial (NCT03124108) of the efficacy of elafibranor in patients with primary biliary cholangitis has recently begun. These patients with primary biliary cholangitis are under-responsive to ursodeoxycholic acid. Whether this can reflect the antifibrotic effect is also awaited.

The other most promising categories of antifibrotics for NASH are FGF19 analog or FGF21 analog. FGF19 is a hormone that potently regulates CYP7A1-mediated bile acid homeostasis, inhibits fatty acid synthesis and increases fatty acid oxidation to maintain glucose homeostasis. Aldafermin (formerly NGM282), a FGF19 analog, treatment induced histological improvement in patients with NASH who received subcutaneous 3 mg of aldafermin once daily for 12 weeks (-0.5 fibrosis score, p=0.035). Significant reductions in fibrosis scores and neoepitope-specific N-terminal propeptide of type III collagen are evident in 12 weeks (-22% and -33% in the 1 mg and 3 mg groups, respectively).28 At least one adverse event (AE) occurred in 93% of patients in the phase II trial for the treatment of NASH. Injection site reactions (34%), diarrhea (33%), abdominal pain (18%), and nausea (17%) were the most common AEs.29 Some results were also found in patients with primary sclerosing cholangitis after treatment with NGM282. Enhanced liver fibrosis scores were improved in 12 weeks (−0.29, p=0.028, in the 1 mg group; −0.37, p=0.009, in the 3 mg group); however, gastrointestinal symptoms were more frequent in the phase II trial.30 Also, pegbelfermin, an FGF21 analog, can regulate energy metabolism. In a phase IIa trial, 75 patients with NASH with fibrosis stages 1-3 were treated with pegbelfermin 10 mg or 20 mg once per day. Data from 16 weeks of treatment showed that pegbelfermin led to a decrease in liver stiffness (-6.8%, p=0.0004, in the 10 mg group; -5.2%, p=0.0008, in the 20 mg group). However, some common AEs occurred (16% diarrhea, 14% nausea).31 Although these side effects are mild, further observation is needed in future trials.

Although liver injury caused by different etiologies determines the initial mode of the liver fibrosis response, the pathological mechanism of fibrosis caused by different injury factors in the late stage of fibrosis is relatively consistent, such as bridging fibers between portal vein regions and cirrhosis.32 In any case, removal of the causative factor, such as weight loss in NAFLD or suppression of viral replication in hepatitis B/C, is the basic treatment strategy to stimulate regression or reverse fibrosis. It should be noted that fibrosis may continue to progress in some patients in whom we are able to control or cure the primary disease,17^,19 although the control of the primary disease is very effective in the treatment of fibrosis. Therefore, the mechanism of liver fibrosis still needs to be studied, while other strategies for the treatment of liver fibrosis still need to be carried out.

Control of the inflammation

The inflammatory response activates a variety of inflammatory cells and releases inflammatory cytokines, which makes HSCs change from a static state to an activated and proliferative state. Furthermore, it can lead to the deposition of ECM, and at the same time, it can also cause the disorder of liver immune function and further aggravate the injury of hepatocytes. Therefore, the inhibition of inflammation and the immune response are also important links in the treatment of fibrosis. Targeting inflammatory mediators or inhibiting the infiltration of inflammatory monocytes can reduce the formation of fibrosis.

Chemokines released by stress hepatocytes, Kupffer cells, endothelial cells and HSCs can regulate the recruitment of inflammatory cells (monocytes, neutrophils, lymphocytes) in the liver.33 The chemokine receptor CC chemokine receptor 2 (CCR2) is one of the core drivers of hepatic inflammation and fibrosis.34^,35 It has been observed that the degree of hepatic fibrosis can be inhibited by targeting CCR2 in patients with NASH.36 The chemokine receptor CCR5 also contributes to fibrosis.35

Cenicriviroc, a dual inhibitor of CCR2/CCR5, has been tested in patients with fibrotic NASH, producing exciting results. In phase IIb trials (CENTAUR), a total of 126 patients with NASH with bridging fibrosis and/or NAS ≥5 treated with cenicriviroc 150 mg were observed to yield a reduction in fibrosis. Even though antifibrotic effects have been reported, the safety of cenicriviroc should be considered carefully, as 2.8% of patients experienced fatigue and 2.1% of patients experienced diarrhea in 289 patients at year 1.37 The antifibrotic effect of cenicriviroc was also shown in the final data at year 2.38 In addition, a phase III trial (AURORA, NCT03028740) is ongoing, and reports of the side effects of cenicriviroc are worthy of continuous follow-up. In the same way, cenicriviroc can also inhibit inflammation and reduce fibrosis by inhibiting hepatocyte death,39 balancing metabolism,40 or regulating the “gut-liver axis”,41 which are also promising treatment strategies.

Other drugs aimed at blocking the recruitment of inflammatory cells such as macrophages, antioxidants, and hepatoprotectants are also in full-swing preclinical trials and may enter clinical development in the near future.

Inhibition of cellular signaling pathways and cytokines to interfere with or block the activation of HSCs

Many experiments have demonstrated the biological efficacy of fibrogenic cytokines that act in an autocrine or paracrine manner. In particular, TGF-β is a master profibrogenic cytokine. The TGF-β proteins comprise 3 isoforms: TGF-β1, 2, and 3. Mechanistically, TGF-β1 is the predominant isoform in the pathogenesis of liver fibrosis.42 With activated canonical TGF-β signaling, targeted HSCs are transdifferentiated to MFs, inducing ECM production. In fact, inhibiting the overexpression and activity of TGF-β has become a promising target of antifibrosis therapy.43^,44 However, inhibition of TGF-β almost acts ubiquitously in all organisms, which may induce inflammation or tumors. Thus, limiting TGF-β to directed fibrotic organs has become a challenge.45 These emerging cellular and signaling pathway mechanisms of liver fibrosis or cirrhosis provide the basis for research on antifibrotic strategies.

In addition, TGF-β’s ligand-receptor binding and its signal transduction pathway may become potential targets for antifibrosis therapy. Research on such is in full-swing, and many experiments and clinical trials have already demonstrated that fibrosis can be slowed or reversed by inhibiting the activation of HSCs and regulating the signal-related pathway. Among them, cannabinoid receptor 1 (CB1) antagonist, angiotensin-converting enzyme inhibitor or angiotensin II receptor 1 blocker, endothelin 1 receptor antagonist, tyrosine kinase inhibitor, FXR antagonist, PPAR agonists, vitamin D receptor,46 and adiponectin have shown potential for antifibrotic therapies. In particular, OCA (an FXR agonist) has demonstrated clinical benefit among patients with NASH in phase III clinical trial,47 pioglitazone (a PPARγ antagonist),48 rimonabant (CB1 antagonist) and other drugs are also undergoing trials.49

OCA, as described above, might be the most promising drug candidate that reduces fibrosis in patients with NAFLD. Although treatment of patients with NASH with OCA given orally at 25 mg daily for 72 weeks was found to be safe in a phase II (FLINT) trial; of note, 33 (23%) of 141 patients in phase II developed pruritus. Moreover, pruritus in the REGENERATE trial (phase III, NCT02548351) in 1968 patients with NASH with stage F1-F3 fibrosis accumulated (p=0.0002). Moderate to severe pruritus occurred in 336 (51%) patients in the OCA 25 mg group.47 Other AEs caused by OCA are elevated total cholesterol or low-density lipoprotein. The study of the efficacy and safety of OCA in patients with NASH with compensated cirrhosis in the phase III trial (REVERSE, NCT03439254) is ongoing. Thus, long-term efficacy and safety treatment with OCA need to be further considered.

Although studies of fibrosis pay close attention to intrahepatic cells and signaling pathways, it is important to realize that hepatic fibrosis is also greatly affected by extrahepatic events, including signals from the gut, fat, and muscles. All targeted therapies are effective in preclinical studies. The reason may be that the target is clear, but the actual clinical requirements for drug side effects are also very high and there will be a compensatory mechanism when a single target is blocked. Therefore, a very effective target drug has not been found and commercialized as antifibrotic therapy. In the future, in addition to further intervention with effective targets, combination therapy may also be a possible direction.

Clearance of activated HSCs

Promoting the apoptosis of activated HSCs, deactivation or direct reduction in the number of MFs may prevent the progression of liver fibrosis. The increased expression of nuclear factor-κB (NF-κB) and the antiapoptotic protein Bcl-2 can lead to the continuous activation of HSCs.50 Drug-induced apoptosis of HSCs by inhibiting NF-κB, including fraxetin (7,8-dihydroxy-6-methoxy coumarin)51 and 4-hydroxy-2(3H)-benzoxazolone,52 has been identified in many animal experiments. Although these drugs may be potential antifibrotic agents, clinical trials have not yet begun. Therefore, it may be a long time before it can be used in the clinic. The clearance of HSCs or MFs by apoptosis can be controlled therapeutically. Recently, a novel molecular therapy that modulates Bcl-x alternative splicing by an antisense oligonucleotide to induce HSC apoptosis may become a potential antifibrosis treatment strategy.53

In research, apoptosis signal-regulating kinase 1 (ASK1) can regulate the key apoptosis pathway of HSCs and hepatocytes, as well as the inflammatory signal.54 Selonsertib can reduce the activation of HSCs, collagen production, activation of inflammatory cytokine pathways and oxidative stress by inhibiting ASK-1.55 There is heartening evidence that 24 weeks treatment with selonsertib (6 or 18 mg, orally once daily) leads to improvement in fibrosis in patients with NASH with stage 2 or 3 fibrosis in a phase II trial. The proportion of patients with a ≥1 stage improvement in fibrosis was 43% (13/30 patients) in the 18 mg selonsertib group and 30% (8/27 patients) in the 6 mg selonsertib group.54 Worryingly, many patients experienced at least one or more AEs. The most common AEs were nausea, headache, nasopharyngitis, upper abdominal pain, sinusitis, back pain, and fatigue. In fact, 6.9% of patients experienced serious AEs (5/72), and 4.2% of patients discontinued treatment because of AEs (3/72).54 However, the phase III study of selonsertib (STELLAR-3 and STELLAR-4) did not meet the primary endpoint that fibrosis stage improvement without the progression of NASH. Selonsertib also did not reduce fibrosis in NASH patients with bridging fibrosis (F3) or compensated cirrhosis (F4) versus placebo at week 48. In STELLAR-3, the primary endpoint was achieved in 12% (p=0.93) of NASH patients with F3 in the selonsertib 6 mg group and 10% of patients (p=0.49) in the selonsertib 18 mg group. In STELLAR-4, the proportion was 14% (p=0.56) and 13% (p=0.93) in patients with F4, respectively.56 The present data showed no effect on reversing advanced fibrosis, while the serious AEs may also not be conducive to the promotion of drugs.

As an important part of innate immunity, natural killer (NK) cells can kill activated HSCs to enhance the immune surveillance ability of NK cells and activate their scavenging and killing effects, which could be an approach to scavenge activated HSCs.57 Therefore, the expansion of NK cells may be a new method for the treatment of liver fibrosis. However, hyperactivated NK cells can also lead to the progression of fibrosis by enhancing inflammation in the liver.58 Thus, understanding the balance of NK cells in regulating HSCs in patients with chronic liver disease can help us design novel antifibrotic therapies. The production of interferon-γ is a marker of NK cell activation and a potent antifibrogenic cytokine contributing to inhibiting fibrogenesis via NK cells. Although the systemic use of interferon-γ has no positive results and interferon-γ-related side effects are inevitable, engineered targeted interferon-γ offers new hope as it can inhibit the activation of HSCs in carbon tetrachloride-induced fibrosis in a mouse model but does not induce related side effects.59

Inhibition of the production of ECM and promotion of degradation

Emerging antifibrosis therapy aims to inhibit the production of ECM and/or prevent the deposition of ECM protein. ECM is a critical determinant of cell and tissue function in fibrosis. Matrix metalloproteinases (MMPs) are a family of more than 24 zinc-dependent endopeptidases that can degrade any component of the ECM.60 According to their ECM substrate specificity, MMPs have been divided into five categories: gelatinases, collagenases, membrane-type, matrilysins, and stromelysins.61 MMPs can not only degrade ECM proteins but also act on non-ECM substrates, such as chemokines and cytokines, which can modulate cell inflammation.62 In the liver fibrosis rat model, carbon tetrachloride and bile duct ligation confirmed that suppressed tissue inhibitor of metalloproteinase (TIMP)-1 expression can inhibit the formation of liver fibrosis by promoting ECM degradation.63

TIMP is a family of at least four physiological inhibitors (TIMPs 1-4) that can regulate proteolytic activity in tissues. Chronic inflammation and repeated repair processes lead to excessive accumulation of ECM components, such as collagen, fibronectin and proteoglycans, which are major participants in the formation of scar tissue. Both MMPs and TIMPs are considered to play central roles in the development of liver fibrosis at different time periods. Basic studies have shown that the balance between MMPs and TIMPs plays an important role in the homeostasis of ECM content. In addition, the expression and activity of MMPs and TIMPs are necessary to ensure fibrinolysis during the regression of fibrosis. These are expected to become therapeutic targets for new drugs.

Similarly, lysyl oxidase-like protein 2 (LOXL2) can promote the cross-linking and stabilization of type I collagen, which is the key to the progression or regeneration of fibrosis. Some experiments have shown that it is effective to use the inhibitory monoclonal LOXL2 antibody AB0023 for early treatment in a mouse model of mild liver fibrosis.64 Although these studies have shown that targeted LOXL2 inhibition is one of the treatments for the prevention or regression of liver fibrosis, it still needs to be tested in clinical trials. Simtuzumab (formerly GS-6624), a monoclonal antibody directed against the LOXL2 enzyme produced by Gilead Sciences, has completed a clinical trial in human immunodeficiency virus- and/or HCV-infected adults with liver fibrosis. However, there was no significant improvement the Ishak fibrosis stage after simtuzumab treatment for 96 weeks (p=0.12 vs. placebo, in the 75 mg Arm; p=0.13 vs. placebo, in the 125 mg Arm).65

Studies have shown that pirfenidone can effectively reduce the expression of heat shock protein-47 (HSP-47) and reduce the abnormal accumulation of collagen I and collagen III and down-regulate the expression of collagen II, TIMP-1 and MMP2 by regulating the activity of the TGF-β signaling pathway, effectively reducing collagen deposition by 70%, inhibiting HSC proliferation and serum transaminase levels, and preventing balloon degeneration of hepatocytes. Pirfenidone treatment reduces liver inflammation and fibrosis in patients with HCV. The Ishak fibrosis stage improved two points in 67% (p<0.05) of patients with chronic hepatitis C after receipt of study drugs at the 24-month point.66 Despite the encouraging results, there are still concerns about the potential AEs associated with pirfenidone.67 In reports on the treatment of pulmonary fibrosis with pirfenidone, the median time to develop an AE after the use of pirfenidone was 15 days.68 Gastrointestinal symptoms (nausea, vomiting, and diarrhea) and skin-related AEs (rash and photosensitivity) are the most common AEs caused by pirfenidone. Liver function AEs and fatigue associated with the treatment of pirfenidone also need to be monitored.69 Thus, further clinical trials are needed to confirm the safety in patients with fibrosis. But more worrying is that pirfenidone exhibited less of an antifibrotic effect in advanced liver fibrosis.70^,71

HSP-47 plays a conclusive role in the secretion and maturation of collagen and other ECM. BMS-986263 is a targeted lipid nanoparticle delivering HSP-47 small interference RNA. Recently, the efficacy of BMS-986263 was announced at the 2019 meeting of the American Association for the Study of Liver Diseases. Although with a limited number of participants in this trial, the Ishak fibrosis stage was improved in the patients with advanced fibrosis after cure of HCV at week 12 (NCT03420768).

It is critical to recognize that preventing the inhibition of ECM and promoting its degradation can help the treatment of patients with advanced fibrosis or cirrhosis. Continued experimental advances are flourishing, yet most of these studies have not been carried out in humans. Therefore, there may be a long way to go to develop effective antifibrotic drugs by halting the progression or inducing the regression of ECM proteins.

Summary and perspective

Pharmacotherapy for hepatic antifibrotic continues to represent major unmet medical needs. We have summarized the major targets for the most promising pharmacological agents in clinical trials in Fig. 3. Although with different shortcomings, a number of drugs have been investigated in phase III clinical trials and provide great hope for antifibrosis therapy in the future. The research and development of newly emerging pharmaceutics targeting different signaling pathways and targets will be helpful to reduce the burden of chronic liver disease and will reduce the number of hepatic decompensations or HCC. In addition, some traditional Chinese medicines, such as Fuzheng Huayu,72 Biejia Ruangan73 and Ganshuang granules,74 have gratifying antifibrosis effects in China. The effect of these traditional Chinese medicines on reducing liver fibrosis has also been confirmed.75 However, the treatment of advanced fibrosis and liver cirrhosis may still take longer to complete reversal, and drug research for patients with irreversible liver cirrhosis is also a challenge.

Fig. 3. Major targets for the most promising pharmacological agents in clinical trials.

Abbreviations: CCR, CC chemokine receptor; ECM, extracellular matrix; ER, endoplasmic reticulum; FGF, fibroblast growth factor; FFA, free fatty acid; FXR, farnesoid X receptor; HSC, hepatic stellate cell; HSP, heat shock protein; JNK, c-Jun N-terminal kinase; PPARs, peroxisome proliferator-activated receptor; ROS, reactive oxygen species; SREPB1, sterol regulatory element binding protein 1.

The emergence and development of fibrosis is a multifactor, multistep complex process, so it may be difficult to make a breakthrough in the treatment of a single target, a pathway, or a single link. Thus, it is an ideal option to develop a combination therapeutic strategy on multiple pathways. The combination of drugs should involve therapy strategies for curing or controlling the primary disease along with direct as well as indirect antifibrotic approaches. Anyhow, it can be expected that research on antifibrotic drugs will continue to be popular for a long time in the future. We will hopefully witness the success of the strategy of hepatic antifibrotic therapy for further improving the effectiveness and safety of treatment to improve outcomes in the near future.

Abbreviations

AE

adverse event

ASK1

apoptosis signal-regulating kinase 1

CB1

cannabinoid receptor 1

CCR

CC chemokine receptor

ECM

extracellular matrix

FGF

fibroblast growth factor

FXR

farnesoid X receptor

GLP-1

glucagon-like peptide-1

HBV/HCV

hepatitis B or C virus infection

HSC

hepatic stellate cell

HSP-47

heat shock protein-47

LOXL2

lysyl oxidase-like protein 2

MF

myofibroblast

MMP

matrix metalloproteinase

NA

nucleos(t)ide analog

NAFLD

nonalcoholic fatty liver disease

NASH

nonalcoholic steatohepatitis

NF-κB

nuclear factor-κB

NK

natural killer

OCA

obeticholic acid

PDGF

platelet-derived growth factor

PPAR

peroxisome proliferator-activated receptor

TGF

transforming growth factor

TIMP

tissue inhibitor of metalloproteinase