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. Author manuscript; available in PMC: 2020 Aug 21.
Published in final edited form as: Nature. 2019 Nov 27;577(7790):426–431. doi: 10.1038/s41586-019-1820-0

Figure 1: FACT forms two complexes with a partially assembled nucleosome.

Figure 1:

a. Domain structure of the two FACT subunits SPT16 and SSRP1. NTD – N-terminal domain; DD – dimerization domain; MD – middle domain; CTD – C-terminal domain; IDD – intrinsically disordered domain; HMG – HMG box domain. Domains for which no density is visible shown in white.

b. Biochemical characterization of FACT-sub-nucleosome complexes by native- and SDS-PAGE, stained by SYBR Gold and Blazin Blue. This experiment was repeated more than 10 times with similar results.

c. Ribbon diagram of DNA, histones and FACT domains fit into class 1 (top) and class 2 (bottom) electron density maps (80% transparency).

d. Schematic representation of sub-nucleosomal particles and FACT (top row), and of the two complexes in the same orientation as in c).