Table 2.

Oligonucleotide primers used in this study to amplify and sequence Alexandrium SSU, ITS1, 5.8S, ITS2, and the D1–D3 domain of the LSU.

Primer name	Sequence (5′−3′)	Melting temperature, °C
PCR amplification primers
Alex1600F	GCYTGAGTCATCAGCTTGTGC	64
LSUB	ACGAACGATTTGCACGTCAG	60
Sequencing primers
5.8SF	CATTGTGAATTGCAGAATTCC	58
Alex5.8SR	GCTCACGGAATTCTGCAATTC	62
AlexLSURl	CCACCCACTTTGCATTC	52
D1R	ACCCGCTGAATTTAAGCATA (Scholin et al. 1994)	56
M13F	GTAAAACGACGGCCAG	50
M13R	CAGGAAACAGCTATGAC	50