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. 2020 Jul 28;20(4):3336–3342. doi: 10.3892/etm.2020.9059

Identification of a CCDC114 variant in a Han-Chinese patient with situs inversus

Xiangyu Chen 1,*, Sheng Deng 1,2,*, Hong Xia 3, Lamei Yuan 1, Hongbo Xu 1, Shiyu Tang 1, Hao Deng 1,4,✉
PMCID: PMC7444340  PMID: 32855706

Abstract

The function and position of the internal organs within the human body are based on left-right (LR) asymmetry. Human LR asymmetry disorders are characterized by abnormal LR asymmetric arrangement of the internal organs resulting from defective embryonic nodal cilia and nodal signaling pathway. The coiled-coil domain containing 114 gene (CCDC114) is related to the biogenesis of cilia and attachment of the outer dynein arms (ODAs) to the axoneme of cilia. Mutations in the CCDC114 gene are reported to cause a subtype of primary ciliary dyskinesia (PCD) named ciliary dyskinesia, primary, 20 (CILD20). Patients with CCDC114 mutations present with a type of ciliopathy with high clinical heterogeneity. In the present study, a Han-Chinese patient with situs inversus was recruited. Exome sequencing was performed on this patient combined with variant validation by Sanger sequencing. A homozygous variant c.584T>C (p.L195P) in the CCDC114 gene was identified as the likely genetic cause for situs inversus in this patient. The findings of our study extend the mutational spectrum of the CCDC114 gene, and contribute to clarifying the pathogenesis of human ciliopathies and benefit genetic counseling.

Keywords: coiled-coil domain containing 114, situs inversus, primary ciliary dyskinesia, exome sequencing, variant

Introduction

Internal organs of the human and other vertebrates exhibit a highly conserved left-right (LR) asymmetry arrangement which is the base for their function and position (1,2). The normal organ asymmetry arrangement across the LR axis is termed as situs solitus (3,4). LR asymmetry disorders occur as a result of genetic defects in the motile cilia of the embryonic node and nodal signaling pathway (5,6). The cilia-mediated fluid flow plays a critical role in the establishment of LR asymmetry arrangement during early embryonic development (7). Human LR asymmetry disorders with a minimum incidence of 1 per 8,000 newborns, can be divided into two categories, including situs inversus (complete mirror-image reversal of the internal organs) and situs ambiguus (abnormal arrangement of the internal organs, also named heterotaxy) (1,4). Situs inversus is also named situs inversus with dextrocardia for mirror-image anatomical topography of the heart (8,9). Situs inversus occurs in approximately half of patients with primary ciliary dyskinesia (PCD), while situs ambiguus occurs in no less than 12% of PCD patients (8). PCD is a ciliary disorder characterized by LR asymmetry disorder, neonatal respiratory distress, male infertility, and chronic oto-sino-pulmonary disease (10,11). Other rare symptoms including female infertility, hydrocephalus and retinitis pigmentosa have also been observed (6,12). Most of PCD cases are inherited in an autosomal recessive inheritance pattern, and a few have autosomal dominant or X-linked inheritance (8,13). Mutations in the genes associated with the establishment and function of nodal cilia are one of the genetic causes of human LR asymmetry disorders (4,12). No less than 69 genes have been reported to be associated with human LR asymmetry disorders (5,13-22). Mutations in the coiled-coil domain containing 114 gene (CCDC114), encoding a ciliary protein necessary for the attachment of the outer dynein arms (ODAs) to the axoneme of cilia, are reported to cause a subtype of PCD named ciliary dyskinesia, primary, 20 (CILD20) (23).

In the present study, a combination of exome sequencing and Sanger sequencing was used to identify the disease-causing gene and variant for a Han-Chinese patient with situs inversus. As a result, a homozygous variant, c.584T>C (p.L195P), in the CCDC114 gene, was identified in the patient with situs inversus.

Patient and methods

Participators and clinical data

A 62-year-old female patient diagnosed with mirror-image dextrocardia using a 12-lead electrocardiogram, in addition to a 59-year-old unrelated healthy female without any off-target condition, were recruited from the Third Xiangya Hospital, Central South University (Changsha, China) in December, 2018. The medical record of the patient was scrutinized and routine physical and radiological examinations were performed on the patient. The medical history of her deceased parents was also scanned. Written informed consent was obtained from the patient and the healthy individual before venous blood was sampled. The entire study was approved by the Institutional Review Board of the Third Xiangya Hospital, Central South University (Changsha, China) and carried out in accordance with the Declaration of Helsinki as revised in 2013.

Exome capture

After being extracted from peripheral blood samples by using the standard method described in a previous study (24), the genomic DNA (gDNA) of the proband was quantified by Qubit dsDNA HS Assay Kit (Invitrogen; Thermo Fisher Scientific, Inc.), and its integrity and purity were qualified on 1% agarose gel. An exome library was constructed with 1 µg gDNA. It was sheared into fragments using Covaris E220 (Covaris, Inc.) and those with sizes of between 150 and 250 bp were selected using Agencourt AMPure XP Kit (Beckman Coulter Inc.). After being subjected to end-repairing, A-tailing reactions and adaptor ligation, the ligated fragments were enriched via amplification, purification and hybridization, using Agilent SureSelect Human All Exon V6 (cat. no. 5190-8872; Agilent Technologies, Inc.) and Agencourt AMPure XP Kit (Beckman Coulter Inc.). Following circularization with T4 DNA Ligase (cat. no. L6030-LC-L; Enzymatics Inc.; Qiagen GmbH), captured enrichment was processed by rolling circle amplification to form DNA nanoballs, which were then loaded onto a sequencing chip with a concentration of 40 ng/µl to be sequenced using MGISEQ-2000RS High-throughput Sequencing Set (FCL PE100; cat. no. 1000012554; BGI group). Paired-end sequencing with read lengths of 100 bp was performed on BGISEQ-500 sequencing platforms (BGI group) using combinatorial probe-anchor synthesis. All of the aforementioned experimental operations were performed in accordance to the manufacturers' protocols.

Read mapping and variant analysis

The raw data was processed to clean data which was mapped to the human reference genome (GRCh37/hg19) using Burrows Wheeler Aligner (BWA; version 0.7.15) (25). Remove of duplicated sequence by Picard (version 2.5.0, https://broadinstitute.github.io/picard/) and realignment by Genome Analysis Toolkit (GATK v.3.3.0, https://gatk.broadinstitute.org/hc/en-us) were performed for accurate variant calling (26). Insertions-deletions (indels) and single nucleotide polymorphisms (SNPs) were called using HaplotypeCaller of GATK. Variants were annotated by SnpEff software (http://snpeff.sourceforge.net/SnpEff_manual.html) (27), and filtered against public databases including the Single Nucleotide Polymorphism database (dbSNP build 141) (28), 1000 Genomes Project (29) and the NHLBI exome sequencing project (ESP) 6500(30), and an in-house exome database of BGI-Shenzhen (BGI group). The causative variants were confirmed by Sanger sequencing with ABI 3500 sequencer (Applied Biosystems; Thermo Fisher Scientific, Inc.) (31). The following are the sequences of primers for potential causative variant in the patient: Forward, 5'-CAGTCCAGCCTCCAGTCATC-3' and reverse, 5'-TTTTCACGCTTCTCCAGGAC-3'.

Bioinformatic analyses

The possible impacts of variants on protein structure or function were predicted by several bioinformatic prediction software programs including MutationTaster (32), Protein Analysis Through Evolutionary Relationships (PANTHER) (33) and Protein Variation Effect Analyzer (PROVEAN) (34,35). A further conservative assessment of the amino acid at the variant position among different species was performed with the National Center for Biotechnology Information-Basic Local Alignment Search Tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi) (36,37). Protean program of DNAStar's Lasergene v7.1.0 (DNASTAR, Inc.) was used to predict whether the candidate variant affected the protein secondary structure (38).

Quantitative PCR (qPCR)

qPCR was performed to check the possible deletion involving candidate variant position using a LightCycler® 480 Instrument II (Roche) with qPCR SYBR Green Master Mix (Vazyme Biotech Co., Ltd.). The primers for the possible deletion were as follows: 5'-CAGTCCAGCCTCCAGTCATC-3' and 5'-ACCTGCGCCTCCATCTCG-3'. The gDNA sample of the healthy individual was used as the reference control. The glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) served as the reference gene and the primers were: 5'-CACTCCTCCACCTTTGACGC-3' and 5'-CCACCACCCTGTTGCTGTAG-3'. Each reaction was conducted in triplicate. A repeated experiment was performed with the reference ATP binding cassette subfamily A member 4 gene (ABCA4) to ensure repeatability, and the primers were as previously published (39): 5'-ACCCAAGTATGGCCCGTCCA-3' and 5'-TCCCATCCATCTGTTGCAGG-3'. The relative copy numbers of the candidate gene were evaluated using the comparative quantification cycle (2-ΔΔCq) method (40). Statistical analysis was performed using the Microsoft Excel 2016 software (Microsoft, Inc.) and GraphPad Prism v8.0.2 (GraphPad Software, Inc.) using a two-tailed unpaired Student's t-test.

Results

Clinical findings

Chest radiograph revealed the mirror-image reversal of the heart and gastric bubble in the patient (Fig. 1), and no other classical PCD symptoms were observed, which agreed with the diagnosis of situs inversus (Table I). The medical history of her deceased parents suggested a negative family history in her family (Fig. 2A).

Figure 1.

Figure 1

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Chest X-ray of the patient with situs inversus.

Table I.

Clinical characteristics and genotypes of patients with CCDC114 variants.

Study (ref.) Patient Sex Age (years) Situs Neo RDS Bxsis Sinusitis Otitis media Other symptoms Variant Zygosity
Onoufriadis Family 1 II:3 M NA SS − + + + − c.742G>A Homozygote
et al, 2013   II:4 M NA DEX NA + + − −    
(23)   II:7 F NA NA NA NA NA NA NA    
    III:1 M NA SS + NA + + CHD    
    III:2 M NA SI − NA + + −    
    III:3 M NA SI − NA − + −    
    III:5 F NA SS − + − + −    
    III:8 M NA SS − NA − + −    
  Family 2 II:2 F NA SS − + + + −    
  Family 3 I:1 F NA SS NA + + + −    
  Family 4 II:1 M NA SS − NA − + −    
    II:2 F NA Abdominal SI − NA − + −    
  Family 5 II:1 F NA SS − NA NA + −    
  Family 6 II:1 M NA SS − NA + + −    
  Family 7 II:1 M NA SI with medial heart position − NA − + CHD    
  Family 8 II:1 F NA SI + NA − − −    
  Family 9 II:1 F NA SI − + + + CHD c.486+1G>A Homozygote
Knowles et al Family 10 III:1 F 39 SS + + + + − c.742G>A Homozygote
2013(55)   III:3 F 33 SS + + + + −    
  Family 11 I:3 F 59 SS + + + + − c.742G>A; Compound
                      c.487−2A>G heterozygote
  Family 12 IV:3 M 3 SS + − − + − c.742G>A; Compound
                      c.1391+5G>A heterozygote
  Family 13 II:1 F 38 SS − NA NA + − c.742G>A; Compound
                      c.939delT heterozygote
    II:2 F 34 SS − + + + −    
Li et al, 2019(52) Family 14 III:1 F 15 SS − + + − Multiple organ dysplasia, renal fibrosis and CHD c.596C>T (p.A199V) Homozygote
Present study Family 15 II:1 F 62 SI − − − − − c.584T>C (p.L195P) Homozygote
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CCDC114, the coiled-coil domain containing 114 gene; M, male; F, female; SS, situs solitus; SI, situs inversus; DEX, dextrocardia; Neo RDS, neonatal respiratory distress; Bxsis, bronchiectasis; CHD, congenital heart disease; +, present; –, absent; NA, not available.

Figure 2.

Figure 2

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(A) Pedigree of a Han-Chinese patient with situs inversus. Square represents male; circles represent females; slashed symbols represent deceased individuals; darkened symbol symbolizes patient with situs inversus; arrow presents the proband. (B) Sequence of the homozygous c.584T>C (p.L195P) variant in the proband. (C) Sequence of the wild-type CCDC114 gene in the healthy individual. CCDC114, the coiled-coil domain containing 114 gene.

Variant screening

A total of 142.67 million effective reads were generated from exome sequencing of the patient. Among them, 99.94% were successful in mapping to the human reference genome. The target sequence covered 99.48% of bases at ≥x10 with an average sequencing depth of x177.41. A total of 97,358 SNPs and 16,875 indels were detected in the patient. A variant filtering strategy as described in recent studies was utilized for confirming the potential causative variant for the patient (39,41): i) Variants documented in dbSNP141, 1000 Genomes Project and NHLBI ESP6500 with a minor allele frequency >0.1% were excluded; ii) among the remaining, variants absent in 1,943 additional Chinese controls without LR asymmetry disorder from an in-house exome database of BGI-Shenzhen were preferred for the next analysis; and iii) variants predicted to be deleterious by in silico tools were reserved. Using the aforementioned criteria, a variant in the CCDC114 gene (reference sequence: NM_144577.4), c.584T>C (p.L195P), was selected as the most possible disease-causing variant for the patient. Sanger sequencing validated the variant in the patient (Fig. 2B) and its absence in the healthy individual (Fig. 2C). The qPCR analysis confirmed no deletion of the CCDC114 gene involving the variant position in the patient (Fig. 3).

Figure 3.

Figure 3

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Relative quantification of the CCDC114 gene in the patient and a healthy individual by genomic quantitative PCR. CCDC114, the coiled-coil domain containing 114 gene.

Bioinformatic analyses

The CCDC114 variant c.584T>C (p.L195P), was predicted to be disease-causing by MutationTaster with a probability score of ~1, possibly damaging by PANTHER with the position in the protein evolutionarily conserved for >200 million years (preservation time, 220 million years), and deleterious by PROVEAN with a score of -5.99. These indicated that the protein structure and function were possibly affected by this variant, which is consistent with the high conservation of the p.L195 residue among nine vertebrates (Fig. 4A). This variant was predicted to change the secondary structure of CCDC114 by Protean based on the Chou-Fasman method (Fig. 4B). An α-helix (residues 181-230) was predicted to be broken into two α-helices (residues 181-193 and residues 196-230) by the c.584T>C variant. Based on the above evidence, the variant c.584T>C in the CCDC114 gene appears to be accountable for the situs inversus in this patient.

Figure 4.

Figure 4

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(A) Conservation analysis of the p.L195 residue in CCDC114 among nine different vertebrates. (B) The α-helix conformations of wild-type and mutant CCDC114 predicted by Protean. An α-helix (residues 181-230) was disrupted at residues 194-195 by the variant. CCDC114, coiled-coil domain containing 114.

Discussion

The normal human body displays an obvious LR asymmetry in the placement and structure of the internal organs (42). The perturbation of human LR asymmetry gives rise to disorders including situs inversus and situs ambiguous (43). The generation of LR asymmetry depends on LR side-specific cascades of gene expression during early embryogenesis. The process can be divided into four distinct phases: i) the bilateral symmetry breaking of the early embryo; ii) asymmetric signals transduced from the node to lateral plate mesoderm (LPM); iii) cascades of LR asymmetric gene expression in the left LPM; and iv) LR asymmetric morphogenesis (5,44).

Abnormal embryonic nodal cilia and perturbation in the nodal signaling pathway are two causes for human LR asymmetry disorders (5). A group of human LR asymmetry disorders resulting from dysmotility or absence of embryonic nodal cilia can be regarded as a type of ciliopathy (45). Cilia and flagella are hair-like organelles in eukaryotic cells. A cilium is composed of a basal body, transition zone, axoneme, ciliary membrane, and the ciliary tip. The axoneme is the main part of the cilium, and the basal body inside the cell plays a role in anchoring the cilium (45-47). The axoneme is assembled by nine peripheral microtubule doublets, surrounding a central pair of microtubules (9+2 pattern) or absence of the central microtubules (9+0 pattern) (48). Each peripheral doublet microtubule possesses an outer dynein arm (ODA) and an inner dynein arm (IDA) (6). The nodal motile cilium with a 9+0 axonemal configuration without central pair existing in the early embryo, has dynein arms and nine peripheral doublets (11). Gene-targeted therapy during early embryogenesis that aimed to normalize the nodal signaling pathway or the function of embryonic nodal cilia may be helpful for rescuing the perturbation of human LR asymmetry.

The CCDC114 gene is located at chromosome 19q13.33 and is composed of 14 exons. The ciliary protein, CCDC114, as a component of ODA docking complex along with CCDC151, armadillo repeat containing protein 4, and tetratricopeptide repeat domain 25, may interact with meiosis specific nuclear structural 1 and dynein axonemal heavy chain 9 to participate in binding ODAs to outer doublet microtubules (17,20,21,23,49). The dynein arms containing ATPase provide a driving force (four-fifths from ODAs) for the sliding of peripheral doublet microtubules which generates ciliary beating (49,50). The genetic deficiency in CCDC114 cause ODA defects, leading to immotile cilia. Impaired motile 9+2 cilia and flagella are responsible for PCD symptoms including neonatal respiratory distress, chronic oto-sino-pulmonary disease, infertility, and hydrocephalus, while human LR asymmetry disorders result from defective embryonic nodal motile (9+0) cilia (6,10). The nodal cilia clockwise rotary generates a leftward flow of extraembryonic fluid, which results in the breakage of the bilateral symmetry (51). Mutations in the CCDC114 gene are known to cause CILD20 (OMIM 615067). Deficiency in CCDC114 not only impairs ODA of motile cilia but also impacts the biogenesis of primary cilia (23,52).

Various cases with mutations in ciliary protein and displaying partial PCD symptoms but imperfect for the standard clinical diagnostic criteria for PCD have also been reported (14,17). In the present study, a patient presented with situs inversus but no other PCD symptoms were reported, and a missense variant c.584T>C (p.L195P) located in the coiled-coil domain of the CCDC114 protein was identified in her. The qPCR analysis confirmed no deletion of the CCDC114 gene involving the variant position, validating the homozygosity of the c.584T>C variant. Although consanguineous marriage was denied in this family, the parents resided within the same village which may imply a possibility of founder effect (53). In silico tools including MutationTaster, PANTHER and PROVEAN predicted that the c.584T>C variant had deleterious effects on protein structure and function. This variant was absent in dbSNP141, 1000 Genomes Project, NHLBI ESP6500 and an in-house exome database of BGI-Shenzhen. It may change the secondary structure of CCDC114 by breaking an α-helix (residues 181-230) at residues 194-195 based on Protean prediction. Further functional studies, as well as co-segregation analyses in more families with situs inversus, are necessary for validating accurate clinical classification of this variant. Defective motile cilia in different tissues and organs underlie different PCD symptoms (54). The patient carrying homozygous CCDC114 variant c.584T>C presented with situs inversus, suggesting that this missense variant mainly affects embryonic nodal cilia. Published articles accessed in the PubMed database reported 6 CCDC114 mutations associated with PCD. Biallelic splice-site mutations in the CCDC114 gene including homozygous mutations (c.742G>A and c.486+1G>A) and compound heterozygous mutations (c.742G>A and c.487-2A>G, c.742G>A and c.1391+5G>A, and c.742G>A and c.939delT) were reported to cause classical PCD phenotype, while a homozygous c.596C>T (p.A199V) mutation was reported to cause PCD symptoms accompanied with other additional symptoms linked with defective primary cilia (Table I; Fig. 5) (23,52,55). No obvious phenotype-genotype correlation in CCDC114 is observed. The phenotypic variation of CCDC114-associated ciliopathies suggested that one or more types of cilia may be involved by a single CCDC114 mutation (52), which may be a random chance confounded by other genetic, epigenetic and environmental factors.

Figure 5.

Figure 5

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Schematic representation of the CCDC114 gene and protein. Numbered boxes indicate exons, and unnumbered boxes indicate introns or intergenic regions. Regions indicated by dashed lines represent out-of-proportion exons or introns. All mutations were described following the CCDC114 isoform (NM_144577.4, NP_653178.3). Three coiled-coil domains (residues 9-155, 183-224, and 302-381), a phosphorylation site (p.S517) and a serine-rich region (residues 590-668) were shown in the protein. CCDC114, the coiled-coil domain containing 114 gene. aa, amino acids.

In summary, using exome sequencing and a significant filtration strategy, it was demonstrated that a CCDC114 variant, c.584T>C (p.L195P), may be responsible for situs inversus in a Han-Chinese patient. To the best of our knowledge, the homozygous c.584T>C variant in the CCDC114 gene is first reported as the disease-causing variant for situs inversus in the present study. Findings from the present study broaden the mutational spectrum of the CCDC114 gene and assist the genetic counseling of human LR asymmetry disorders. In the future, more confirmation and functional studies of CCDC114 mutations, and the establishment of deficient animal models with CCDC114-associated ciliopathies will facilitate an in-depth comprehension of molecular and cellular mechanisms of human ciliopathies.

Acknowledgements

Not applicable.

Funding

This study was supported by the National Natural Science Foundation of China (grant nos. 81670216, 81800219 and 81873686), the Natural Science Foundation of Hunan Province (grant no. 2018JJ2660), the Science and Technology Program of Hunan Province (grant no. 2017SK50131), the Scientific Research Project of Health and Family Planning Commission of Hunan Province, China (grant nos. B20180760 and B20180834), the Lotus Scholars Program of Hunan Province (to HD), the Hunan Provincial Innovation Foundation For Postgraduate (grant no. CX20190252) and the Undergraduate Innovative Training Plan Program of Central South University, China (grant no. XCX20190629).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

XC, SD and HD conceived and designed this study. HXi, ST and HD collected the patient samples and clinical data. XC, SD and HXu performed the experiments; XC, LY and HD analyzed the data. XC, SD and HD wrote the manuscript. All authors read and approved the manuscript and agreed to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work was appropriately investigated and resolved.

Ethics approval and consent to participate

The study was conducted in compliance with the Declaration of Helsinki Principles and with the consent of the Institutional Review Board of the Third Xiangya Hospital of Central South University in Changsha, Hunan, China (approval no. 2018-S400). Written informed consent was obtained from the participants prior to being included in the study.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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