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. 2020 Jul 31;9:e58619. doi: 10.7554/eLife.58619

Figure 5. Expression of N-terminal GFP::FASN-1 or C-terminal FASN-1::GFP CRISPR-Cas9 constructs.

Figure 5.

(A) Expression is prominent throughout the hypodermis (small arrows) and excretory duct cell (larger arrow), but not intestine (int). (B) Expression in developing vulva (arrow). (C) Gonadal sheath expression, with absence of apparent germline expression. (D) Earliest expression is seen in the hypodermis of developing embryos, and hypodermal expression continues throughout development. (E) Expression is apparent in all somatic sheath cells (Sh1–5). (F) Anti-GFP antibody staining of dissected gonad and intestine expressing FASN-1::GFP. DAPI staining shows large nuclei (int) of intestine crossing underneath the gonad. Strong expression is detected in the proximal gonad, less so in distal gonad (dis), but no intestinal expression is apparent. (A–E) DIC images in upper panels, live GFP fluorescence in lower panels. Bars, 20 μm.