Figure 5.
a) Targeted DNP strategy for bacterial lysates. b) Overall signal enhancement of the 13Clabeled lysate. c) Strategy to determine the selective enhancement of Ub-TTz in the lysate. The microwave (MW) off signal was scaled to the microwave on signal so that the glycerol solvent peaks matched in intensity. The leftover enhancement was denoted as A. Then, the microwave off signal was scaled down to reflect the 5% abundance of Ub-TTz in the sample and the intensity of this rescaled spectrum was denoted as B. The ratio of A to B produced the total enhancement on ubiquitin. The selective enhancement is equal to the total ubiquitin enhancement divided by the background enhancement.