Diabetic Kidney Disease: Challenges, Advances, and Opportunities

Abstract

Background

Diabetic kidney disease (DKD) is the most common cause of the end-stage renal disease (ESRD). Regardless of intensive treatments with hyperglycemic control, blood pressure control, and the use of renin-angiotensin system blockades, the prevalence of DKD remains high. Recent studies suggest that the spectrum of DKD has been changed and many progresses have been made to develop new treatments for DKD. Therefore, it is time to perform a systemic review on the new developments in the field of DKD.

Summary

Although the classic clinical presentation of DKD is characterized by a slow progression from microalbuminuria to macroalbuminuria and by a hyperfiltration at the early stage and progressive decline of renal function at the late stage, recent epidemiological studies suggest that DKD patients have a variety of clinical presentations and progression rates to ESRD. Some DKD patients have a decline in renal function without albuminuria but display prominent vascular and interstitial fibrosis on renal histology. DKD patients are more susceptible to acute kidney injury, which might contribute to the interstitial fibrosis. A large portion of type 2 diabetic patients with albuminuria could have overlapping nondiabetic glomerular disease, and therefore, kidney biopsy is required for differential diagnosis for these patients. Only a small portion of DKD patients eventually progress to end-stage renal failure. However, we do not have sensitive and specific biomarkers to identify these high-risk patients. Genetic factors that have a strong association with DKD progression have not been identified yet. A combination of circulating tumor necrosis factor receptor (TNFR)1, TNFR2, and kidney injury molecular 1 provides predictive value for DKD progression. Artificial intelligence could enhance the predictive values for DKD progression by combining the clinical parameters and biological markers. Sodium-glucose co-transporter-2 inhibitors should be added to the new standard care of DKD patients. Several promising new drugs are in clinical trials.

Key Messages

Over last years, our understanding of DKD has been much improved and new treatments to halt the progression of DKD are coming. However, better diagnostic tools, predictive markers, and treatment options are still urgently needed to help us to better manage these patients with this detrimental disease.

Introduction

Diabetic kidney disease (DKD) remains as the most common cause of end-stage renal disease (ESRD) in US and most countries. In recent years, the spectrum of DKD has been evolved. Our understanding of DKD pathogenesis has been also advanced significantly. Sodium-glucose co-transporter-2 (SGLT2) inhibitors have been approved as new drugs to treat patients with DKD. In addition, several new drugs have been in clinical trials with some success. Therefore, we believe that it is the time to write this review to update the current understanding of clinical manifestations, disease progression, pathological changes, and management of DKD patients in the new era.

Good and Bad News in the DKD Epidemiology

Diabetes affects 30.3 million people of all ages representing 9.4% of US population [1] and about 149 million people and 10.9% of the population in China [2] and 415 million worldwide [3]. DKD is the most common cause of the ESRD in the world, and it is associated with increased morbidity and mortality in diabetic patients [4]. Although the incidence rate of chronic kidney disease (CKD) in diabetic patients has decreased in recent years in the US, the prevalence remains high. In 2018, USRDS annual data reported that diabetes accounted for 36% of CKD in the NHANES population between 2013 and 2016, declining from 44% between 2001 and 2004. However, due to the increased number of diabetic patients, the total number of DKD patients was further increased. The numbers of diabetic adults aged over 18 years who began treatments for ESRD also increased significantly from over 40,000 in 2000 to over 50,000 in 2014 [5]. In China, both the incidence and prevalence of DKD have risen dramatically over the last decade. In China Kidney Disease Network 2015 annual data report, DKD is the primary cause of CKD, accounting for 26.96% [6]. The estimated number of diabetic patients with CKD in China reached 24.3 million [7]. Therefore, DKD is a global public health burden.

Albuminuria Is Not Necessarily Associated with a Decline in Glomerular Filtration Rate in DKD

Typical clinical manifestations of DKD include albuminuria, which progresses to macroalbuminuria or overt proteinuria over time, microscopic hematuria, which presents only in a small portion of patients, and a low progression rate of renal function. Classically, DKD is divided into 5 stages. The stages 1 and 2 are preclinical stages, characterized by an increase of glomerular filtration rate (GFR), normoalbuminuria (stage 1) or intermittent microalbuminuria (MA; stage 2), and normal blood pressure. Stage 3 is the onset of clinical stage, characterized by persistent MA, mild hypertension, and a normal or slight decline in GFR. Stage 4 is characterized by macroalbuminuria, hypertension, and further decline of GFR. Stage 5 is the end stage of renal disease.

However, recent epidemiological studies suggest that not all patients with DKD follow the above classification, particularly in patients with type 2 diabetes mellitus (DM). Growing evidence suggests that proteinuria does not always occur preceding the loss of renal function in diabetes, which contradicts the abovementioned conventional progression of DKD [8, 9, 10]. In some patients, the progression of DKD to ESRD may occur without a transition from MA to overt proteinuria. Perkins et al. [11] reported that the early decline of GFR occurs in a large proportion of patients with only MA. In addition, the decline of GFR without albuminuria (nonproteinuric DKD) has been increasingly recognized, especially in type 2 diabetes [12, 13, 14, 15, 16]. Kramer et al. [17] reported that renal insufficiency occurs in the absence of albuminuria and retinopathy among adults with type 2 DM. Population-based studies from western countries suggest that about half of individuals with a decline of kidney function did not have preceding proteinuria or never progressed to proteinuria in type 2 diabetes [18]. Compared with proteinuric DKD, nonproteinuric DKD revealed a weaker association with diabetic retinopathy [19]. All the findings may suggest a nonalbuminuric pathway for the progression of DKD. The overview of DKD is seen in Figure 1.

Fig. 1.

Overview of DKD. DKD, diabetic kidney disease, ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin II receptor blocker; SGLT2, sodium-glucose cotransporter 2; eGFR, estimated glomerular filtration rate; BP, blood pressure.

Identification of High-Risk DKD Patients

DKD is a chronic, progressive disease that develops over time. In the 1970s, the median time to ESRD from the development of overt proteinuria in type I diabetes was 7 years [20] and now is 14 years [21]. The incidence of ESRD in type I diabetes from Finland is now 7.8% at 30 years duration [22]. Much evidence has shown that many patients who are well treated may have relatively stable kidney function or progression at a very slow rate. In 3 recent randomized interventional studies, the rate of decline of eGFR ranged from 0 to 4 mL/min per year [23, 24, 25]. A major factor that may contribute to progression to ESRD in patients with diabetes is acute kidney injury (AKI). Thakar et al. [26] demonstrated that, in a cohort of 4,082 patients with diabetes, single or repetitive episodes of AKI significantly increase the risk of developing advanced CKD. Subsequently, a large prospective study further confirmed that AKI itself can also predict major adverse outcomes including doubling of serum creatinine or ESRD in patients with diabetes [27]. Results of the latest research are in line with the above; they found that AKI events were associated with progression to renal replacement rate and also with a greater severity of subsequent AKI [28]. This may explain why some DKD patients have an early decline of GFR with a minimal amount of albuminuria.

Not all DKD patients have identical progression rates. In general, about one-third of patients will progress to MA in 15–20 years after the diagnosis of diabetes and normoalbuminuria. One-third of patients with MA do not progress after treatments, but another one-third of them will deteriorate to macroalbuminuria within 10 years. In the next 10 years, one-third of those with macroalbuminuria will develop to ESRD. Only one-third of the ESRD patients will be still alive in the following 5 years. Since the progression rate is so different among individual patients with DKD, we need to develop new biomarkers that allow us to predict the progression rate of GFR at the early stage of DKD. When patients of all types of diabetes have been included, the incidence rate of ESRD has been 1–9 per 1,000 patient-years [29, 30, 31, 32]. In type I diabetes patients, the cumulative risk of ESRD and the incidence of renal replacement rate have decreased markedly during the past 5 decades [33] but that is not obvious in type 2 diabetes patients [34]. Risk for developing ESRD over 20 years in type 2 diabetes patients is about 2%. Studies suggest that intensive glycemic control did not reduce significantly clinical renal end points, including doubling of serum creatinine, ESRD, and death from renal disease in type 2 DM [35]. Many studies have been undertaken to develop the biomarkers for early detection of these high-risk DKD patients. MA is a dynamic marker at the early stage of DKD and therefore could not be used to predict the progression of DKD [36]. In addition, progression of DKD is not necessarily associated with proteinuria, and thus, proteinuria is also not a sensitive marker for DKD progression. Although intensive glycemic control is able to reduce nephropathy in both type 1 [37] and type 2 diabetes [38], hemoglobin A1c at the early stage of DKD does not predict the late progression of DKD [39]. As for kidney pathology, structural predictors of loss of renal function in American Indians with type 2 diabetes include mean glomerular volume, glomerular basement membrane (GBM) width, mesangial fractional volume per glomerulus, glomerular filtration surface density, nonpodocyte number per glomerulus, foot process width, podocyte detachment, and endothelial fenestration [40]. However, it is unusual to perform kidney biopsy at early DKD. In addition, an autopsy study suggests that histological findings do not necessary correlate with clinical presentation in diabetic patients [41]. Searching for genetic factors is another important approach. Unfortunately, none of the genetic factors have been found to have a strong association with the progression of DKD. Family Investigation of Nephropathy and Diabetes Research Group recently reported that no genome region showed linkage across all ethnic groups. The Genetics of Kidneys in Diabetes (GoKind) study showed no single major gene that contributes to an increased risk of diabetic nephropathy (DN) emerged from the analysis of their data [42]. APOL1 risk allele, which is highly implicated in African-American nondiabetic kidney disease with an odds ratio of >10, is only weakly associated with DKD. Many groups have investigated blood and urine biomarkers for DKD progression based on the pathogenesis of the disease such as cytokines and growth factors. Serum markers include soluble tumor necrosis factor receptor α (TNFα) receptors, TNFα, interleukin 6 (IL-6) neutrophil gelatinase-associated lipocalin, IL-18 and L-type fatty acid-binding protein of tubular cell injury, serum endothelial nitric oxide synthase, von Willebrand factor and vascular endothelial growth factor, and kidney injury molecular 1 (KIM-1). Urine markers include IL-6, IL-18, monocyte chemoattractant protein-1 (MCP-1) of inflammation, transforming growth factor-β (TGF-β), connective tissue growth factor, isoprostanes and 8-hydroxy-2′-deoxyguanosine, podocyte number or expression of podocyte markers, IV collagen of GBM, YKL-40 (also known as chitinase 3-like 1), and MCP-1 [43]. So far, the best circulating biomarkers that could predict the progression of DKD are tumor necrosis factor receptor (TNFR)1, TNFR2, and KIM-1. Gohda et al. [44] found that circulating TNF receptors 1 and 2 predict the progression to stage 3 CKD in type 1 diabetes. Elevated concentrations of circulating TNFRs in patients with type 2 diabetes at baseline are very strong predictors of the subsequent progression to ESRD in subjects with and without proteinuria [45]. TNFR but not hemoglobin A1c predicts that the progression of CKD may imply the possibility that DKD is an inflammatory disease, which could explain why hyperglycemic control is not enough to control the disease progression. Fornoni et al. [97] investigated the role of local and systemic TNF in podocyte injury and implied a nuclear factor of activated T cells 1/ATP-binding cassette transporter A1-dependent mechanism through which local TNF is sufficient to cause free cholesterol-dependent podocyte injury irrespective of TNF, TNFR1, or TNFR2 serum levels [46]. Further researches are in need to clarify how activation of circulating TNF pathway contributes to DKD. In addition, KIM-1 level at baseline was observed strongly predicted rate of eGFR loss and ESRD during 5–15 years of follow-up in a cohort of patients with type 1 diabetes and proteinuria [44]. This suggests that tubular cell injury occurred early at DKD and was associated with the progression. Coca et al. [47] combined those biomarkers and demonstrated 3-plex biomarker panel (plasma TNFR1, TNFR2, and KIM-1) aided risk-stratification for renal end points (sustained 40% decline in eGFR or ESRD) in type 2 diabetics. In the future, artificial intelligence could be applied to combine clinical parameters with the circulating biomarkers to enhance the predictive value for DKD progression. Identification of such a diagnostic tool will help us to better manage the patients with the early stages of DKD. This includes lifestyle modification, resource allocation, and the use of medications. The progression and related biomarkers of DKD are seen in Figure 2.

Fig. 2.

Progression and biomarkers of DKD. KIM-1, kidney injury molecular 1; TNFR, tumor necrosis factor receptor; GBM, glomerular basement membrane; eGFR, estimated glomerular filtration rate; KW, Kimmelstiel-Wilson; ESRD, end-stage renal disease; MA, microalbuminuria.

New Insights into DKD Pathology

DN is classically characterized by GBM thickening, mesangial expansion, nodular glomerular sclerosis, and tubulointerstitial fibrosis [48]. The traditional paradigm of glomerulus-centered pathophysiology has expanded to the tubule-interstitium and the immune response [49]. In type 1 diabetes patients, Mauer et al. [50, 51, 52] reported that the thickening of GBM is the earliest structural finding occurring in 1.5–2.5 years after the diagnosis of DM and the mesangial expansion with matrix deposition occurred in 5–7 years after diagnosis of DM. In later stages, afferent and efferent arteriolar hyalinosis and Kimmelstiel-Wilson nodules are seen, both of which are considered specific findings of DN. Simultaneously, tubular basement thickening, parallel to GBM thickening, is also observed. As for type 2 diabetes, the pathological changes are more complicated and often classified into 3 categories [53]: class I is the classic diabetic glomerulopathy as described above; class II has predominant vascular and interstitial changes, relatively normal glomerular structure. This group of patients often has an early decline of GFR without significant albuminuria. Clinically, patients have an early decline of GFR without significant proteinuria. Mechanistically, these patients may have unrecognized AKI episodes during the disease progression. Class III is divided into 2 subclasses: IIIa is another glomerular disease superimposed on diabetic glomerular structure (accounting for 19%) and IIIb is non-DKD glomerular disease (with no changes of GBM and mesangial expansion, accounting for 18%). This class accounts for a total of 50% of DKD patients. It is unclear whether diabetes interacts with the primary glomerular disease to accelerate the progression of CKD.

In type 1 diabetes, most patients could have a diagnosis of DKD based on clinical presentation. However, the diagnosis of DKD in type 2 diabetes often requires additional tests including kidney biopsy. Diabetes is a common disease with high coincidence with other nondiabetic CKD. Another interesting observation is that clinical manifestation is not necessarily associated with histological findings in DKD, as suggested by an autopsy study [41]. In that research, 106 of 168 patients had histopathologic changes of DN in the kidney, but 20 of 106 histologically proven DN cases did not present with DN-associated clinical manifestations within their lifetime. At the most clinical practice, patients with atypical clinical features should prompt evaluation for non-DKD by performing kidney biopsy. These atypical presentations are constructed of rapidly decreasing renal function, rapidly increasing proteinuria, active urinary sediment, refractory hypertension, and nephrotic range of proteinuria with normal GFR. Since the generalized vascular disease is common in diabetes, refractory hypertension and/or a severe drop of GFR after renin-angiotensin system (RAS) blockade should prompt consideration of renal artery stenosis. Membranous diseases or minimal change disease should be ruled out from a nephrotic range of proteinuria with normal GFR because these patients could be treated with steroids and immunosuppressive therapy. However, since almost 50% of diabetic patients could have non-DKD glomerular diseases or other glomerular diseases overlapped with DKD, we would suggest that any type 2 DM patients with significant proteinuria should undergo kidney biopsy to rule out these conditions.

Challenges and Opportunities in Developing New Therapies for DKD

Conventional therapy of DKD includes better hyperglycemic control, RAS blockers, and other managements such as lipid-lowering therapy and so on. In humans, RAS inhibition has proved to be the single most effective therapy for slowing the progression of DN [54]. However, 3 randomized, placebo-controlled trials of 256–3,326 patients with type 1 diabetes and normoalbuminuria (RASS [55], EUCLID [56], AND DIRECT [57]) suggest that early therapy in patients, type 1 diabetes is ineffective in preventing the development of MA. In addition, the combination therapy with an angiotensin-converting enzyme inhibitor plus an angiotensin II receptor blocker does not prevent renal disease progression or death, and it increases the rate of serious adverse events such as AKI, hyperkalemia, and hypotension, as shown by the Veterans Affairs Nephropathy in Diabetes study (VA NEPHRON-D) [58]. The continued increase in diabetic ESRD urgently requires additional innovative therapies. A better understanding of the pathogenesis of DKD could help us to identify new drug targets for DKD.

Newly Approved Drugs for DKD Treatment

The recent success of SGLT2 inhibitors as a new therapy for DKD patients is exciting and encouraging news for the nephrologists. SGLT2 normally accounts for approximately 90% of glucose reabsorption in the renal proximal tubule [59], and its inhibitors are used in type 2 DM to treat hyperglycemia by increasing glucose excretion in the urine. SGLT2 inhibitors have been shown to lower body weight, blood pressure, serum uric acid, and glomerular hyperfiltration through increased urinary excretion of glucose and sodium, osmotic diuresis, and improved tubule-glomerular feedback mechanism [60]. In Cherney's study (NCT01392560), they found that short-term treatment with empagliflozin, an SGLT2 inhibitor, attenuated renal hyperfiltration in subjects with type 1 diabetes [61]. In patients with type 2 DM and high cardiovascular risks, empagliflozin is associated with slower progression of kidney disease and lower rates of clinically relevant renal events than placebo when added to standard care [62, 63]. Canagliflozin, another SGLT2 inhibitor, was shown in patients with type 2 diabetes and kidney disease (CREDENCE); the risk of kidney failure was significantly lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years [64]. Compared with glimepiride, canagliflozin 100 or 300 mg/day slowed the progression of renal disease over 2 years in patients with type 2 diabetes, and it may confer renoprotective effects independently of its glycemic effects [65]. In Dekkers research, they demonstrated that 6 weeks of dapagliflozin decreased albuminuria by 43.9% and eGFR by 5.1 (2.0–8.1) mL/min/1.73 m² compared to placebo [66]. In another study (NCT02413398), in patients with type 2 diabetes and CKD 3A, decreases from baseline in eGFR were greater with dapagliflozin than placebo at Week 24 (−2.49 mL/min/1.73 m² [–4.96 to −0.02]); however, eGFR returned to baseline levels at Week 27 [67]. Based on these studies, SGLT2 inhibitors have been approved to treat DKD patients with eGFR >45 mL/min, and therefore, SGLT2 inhibitors should be included as part of standard therapy for patients with DKD. However, attentions should be paid to side effects such as hypoglycemia, urinary and genital infections, elevated liver enzymes, bone fractures, or amputations.

Promising Drugs in Phase III Clinical Trials for DKD Treatment

Glucagon-like peptide-1 (GLP-1) is one of the incretins released from the intestine in response to food intake and it can stimulate insulin secretion. Its level is decreased and its analogs (e.g. liraglutide) have been used in type 2 DM. According to the Liraglutide Effect and Action in Diabetes: Evaluation of Cardiovascular Outcomes Results double-blind trial with 9,340 patients with type 2 DM and high cardiovascular risk, patients with liraglutide had lower rate of new onset of persistent macroalbuminuria and progression of DKD than placebo [68, 69]. In another randomized study of 3,297 patients with type 2 diabetes, patients using semaglutide (another GLP-1 analog) had lower rates of new or deteriorating nephropathy than those with placebo [70]. Dipeptidyl peptidase 4 inhibitors linagliptin and saxagliptin can reduce the amount of albuminuria, but the evidence is less clear than GLP-1 analog [71, 72]. Peroxisome proliferator activated receptor-γ agonist, rosiglitazone, can significantly decrease urinary albumin to creatinine ratio in type 2 diabetes patients after 3 months of therapy [73]. In Agarwal's research, though pioglitazone group had a reduction of 7.2% proteinuria, it was not statistically significant [74]. Avosentan, an endothelin-1 receptor A antagonist, can reduce urinary albumin excretion, but the study was terminated early because of excessive cardiovascular events during the treatment course due to the fluid overload [75]. A recent study in 211 patients showed that atrasentan, a more selective endothelin receptor A antagonist, had a proteinuria-lowering effect without significant accumulation of body fluid [76]. However, this study was terminated early due to the recruitment issue. Future studies are required to confirm whether atrasentan has additional renal protective effects on top of the RAS blockades and SGLT2 inhibitors, the new standard care for DKD patients. Mineralocorticoid receptor antagonists (MRA) exert an antifibrotic and anti-inflammatory effect on the kidneys and other target organs like the heart and vessels [77]. Due to significant side effects associated with the first- (spironolactone) and second- (eplerenone) generation MRA, new MRA such as apararenone (also called MT−3995), esaxerenone, and finerenone have a relatively low risk of hyperkalemia. A clinical trial of MT−3995 in patients with DKD (NCT02676401) is ongoing in Japan (clinicaltrials.gov). Esaxerenone is also being studied in phase II and III randomized clinical trial (RCT), but no results have been published yet [78]. Finerenone has been investigated in several phase II RCTs. In the MRA tolerability study-heart failure (ARTS-HF), finerenone reduced albuminuria in patients with CKD and heart failure and had a lower risk of hyperkalemia than spironolactone [79]. In the more recent ARTS-DN study in patients with type 2 diabetes, macroalbuminuria, and eGFR = or <60 mL/min per 1.73 m², finerenone reduced albuminuria in a dose-dependent manner. The risk of hyperkalemia leading to discontinuation was 2.1, 3.2, and 1.7% in the finerenone 7.5, 15, and 20 mg/day groups, respectively [80]. Two double-blind placebo-controlled phase III RCTs, FIDELIO (NCT02540993) and FIGARO (NCT 02545049) are currently ongoing to examine the effects of finerenone on DKD and cardiovascular outcomes [81, 82]. Pirfenidone, an oral nonspecific antifibrotic agent, was shown to have a mean increase of eGFR after 1 year of therapy in 1,200 mg/day group in a randomized, double-blind, placebo-controlled study with 77 DKD subjects [24]. Another phase III RCT (NCT02689778) to determine the effect of pirfenidone on GFR and albuminuria in patients with DKD is still ongoing. Pentoxifylline, another nonspecific antifibrotic agent, was shown to reduce albuminuria and slow progression of renal disease in patients with type 2 diabetes with stages 3–4 CKD on top of RAS blockade [83]. Two more clinical trials (NCT03625648 and NCT03664414) are ongoing.

Potential Drugs Required Further Validations

Hyperglycemia-associated generation of advanced glycation end products (AGE) and engagement of the receptor for AGE with its ligands can induce oxidative stress and renal inflammation. Pyridoxamine, a member of family of vitamin B6, can remove free radicals and carbonyl products and block synthesis of AGEs. Clinical studies showed that pyridoxamine did not provide a significant renal protection in DKD patients [84], but a significant beneficial effect was observed in a subgroup of DKD population. Therefore, more clinical studies are required to further confirm this finding. Systems biology approach reveals that both NF-kB and Janus kinase (JAK)/signal transducer and activator of transcription pathway are heavily involved in the pathogenesis of DKD. Not only in immune cells, but JAK-signal transducer and activator of transcription also play critical roles in renal cells, including mesangial cells, podocytes, and tubular epithelial cells [85]. This pathway is activated by reactive oxygen species induced by the hyperglycemic state [86]. A phase II clinical trial using Baricitinib, a selective JAK-1 and JAK-2 inhibitor, showed a reduction of proteinuria and expression of several inflammatory markers in patients with DKD [85]. Nuclear factor-2 erythroid related factor (Nrf2)-keep 1 pathway has been also shown to play a major role in the progression of DKD [87]. Pharmacological activation of Nrf2 decreases cytokine production, M1 macrophage accumulation, and the formation of an atherosclerotic plaque lipid core in the experimental model of streptozotocin-induced diabetic mice on an apolipoprotein E-deficient background [88]. In addition, its activation improves the pathological changes in the glomerulus of streptozotocin-induced diabetic mice through a reduction in oxidative stress, TGF-β expression, and extracellular matrix proteins [89]. Bardoxolone methyl reduces the generation of oxidative stress by the activation of Nrf2 and inhibition of NF-kB pathway. The short-term study revealed that the use of bardoxolone methyl can increase the GFR in patients with type 2 DM and impaired renal function but have no influence of albuminuria [23]. However, the large phase III clinical study was terminated early because of more cardiovascular events [90]. Oxidative stress plays a critical role in the pathogenesis of DN [91]. NADPH oxidase (NOX) enzyme isoforms are involved in the production of reactive oxygen species that cause kidney cell injury in DKD [92]. GKT137831, a NOX1/4 inhibitor, has been shown to have a beneficial effect in murine models of DN [92]. However, the phase II trial in patients with T2DM and albuminuria failed to show any significant renal protection [93]. APX-115, a pan-NOX inhibitor, has been shown to have a renal protective effect in an experimental animal model of diabetes [94], but human studies have not been done yet. The proinflammatory chemokine ligand 2, also known as MCP-1, has been implicated in the pathogenesis of DN and has become a novel treatment target. Mouse models treated with NOX-E36, a chemokine ligand 2 inhibitor, exhibited a reduction in albuminuria [95]. A phase II clinical trial demonstrated that treatment of NOX-E36 reduced albuminuria in patients with T2DM and DN [96]. Long-term effects of these medications on renal outcomes and mortality are still in need. All drugs for DKD treatment are shown in Table 1.

Table 1.

Review of drugs for DKD treatments

Categories	Drugs	Studies	Outcomes/status
Newly approved drugs
SGLT2 inhibitor	Empagliflozin	NCT01392560 (clinicaltrials.gov) Wanner et al. [63], 2016	Attenuate renal hyperfiltration in subjects with type 1 diabetes Slower progression of kidney disease
	Canagliflozin	Perkovic et al. [64], 2019 Heerspink et al. [65], 2017	Significantly lower risk of kidney failure Slower the progression of renal disease over 2 years in type 2 diabetes
	Dapagliflozin	Dekkers et al. [66], 2018 NCT02413398 (clinicaltrials.gov)	6 weeks of dapagliflozin decrease albuminuria and eGFR Decrease from baseline in eGFR is greater with dapagliflozin than placebo at week 24 but eGFR return to baseline levels at week 27
Promising drugs in phase III clinical trials
GLP-1 analog	Liraglutide	Marso et al. [68], 2016 Mann et al. [69], 2017	Lower rate of new onset of persistent macroalbuminuria and progression of DKD
	Semaglutide	Marso et al. [70], 2016	Lower rates of new or deteriorating nephropathy
Endothelin-1 receptor A antagonist	Avosentan	Mann et al. [75], 2010	Reduce urinary albumin excretion Terminated early because of excessive cardiovascular events
	Atrasentan	de Zeeuw et al. [76], 2014	Had a proteinuria-lowering effect Terminated early due to the recruitment issue
MRA	Apararenone (MT−3995)	NCT02676401 (clinicaltrials.gov)	Ongoing in Japan
	Esaxerenone	Kolkhof et al. [78], 2017	In phase II and III randomized clinical trial
	Finerenone	Pitt et al. [79], 2013 Bakris et al. [80], 2015 NCT02540993 (clinicaltrials.gov) NCT 02545049 (clinicaltrials.gov)	Reduce albuminuria Reduce albuminuria in a dose-dependent manner Ongoing Ongoing
Antifibrotic therapy	Pirfenidone	Sharma et al. [24], 2011 NCT02689778 (clinicaltrials.gov)	Have an mean increase of eGFR after 1 year of therapy in 1,200 mg/d Ongoing
	Pentoxifylline	Navarro-Gonzalez et al. [83], 2015 NCT03625648 NCT03664414 (clinicaltrials.gov)	Reduce albuminuria, slow progression of renal disease in patients with type 2 diabetes and stages 3-4 CKD Ongoing Ongoing
Potential drugs required further validations
Anti-AGE drugs	Pyridoxamine	Williams et al. [84], 2007	Not provide a significant renal protection in DKD patients
JAK-STAT inhibitor	Baricitinib	Tuttle et al. [85], 2018	A phase II clinical trial showed a reduction of proteinuria
Nrf2 activator	Bardoxolone methyl	Pergola et al. [23], 2011 de Zeeuw et al. [90], 2013	Have no influence of albuminuria Increase the GFR in patients with type 2 DM Phase III clinical study was terminated early because of more cardiovascular events
Nox1/4 inhibitor	GKT137831 APX-115	Gorin et al. [92], 2015 Cha et al. [94], 2017	A beneficial effect in murine models of DN A renal protective effect in an experimental animal model of diabetes
Inhibitor of chemokines cytokines	NOX-E36	Boels et al. [95], 2017 Menne et al. [96], 2017	A reduction in albuminuria in mouse models A phase II clinical trial demonstrated a reduced albuminuria in patients with T2DM and DN

SGLT2, sodium-glucose co-transporter-2; GLP-1, glucagon-like peptide-1; MRA, mineralocorticoid receptor antagonists; AGE, advanced glycation end products; JAK-STAT, janus kinase/signal transducer and activator of transcription; Nrf2, nuclear factor-2; Nox1/4, NADPH oxidase; DKD, diabetic kidney disease; GFR, glomerular filtration rate; CKD, chronic kidney disease; DN, diabetic nephropathy; T2DM, type 2 diabetes mellitus.

Conclusion

DKD remains the most common cause of ESRD in the world including the US and China. DM patients with CKD could have a variety of clinical presentations with or without albuminuria. We need to perform more kidney biopsy for DKD patients with albuminuria because a significant number of DM patients could have non-DKD glomerular disease. The clinical presentation does not necessarily correlate with histological changes in DKD patients. Identification of biomarkers for early detection of DKD patients at high risk for progression could help us to better manage DKD patients and prevent their progression. Identification of SGLT2 inhibitors as a new therapy for DKD has a huge impact, however, is not sufficient to halt the progression of DKD. Therefore, there is an urgent need to better understand the pathogenesis of DKD and develop more drugs to treat these patients.

Disclosure Statement

The authors have no conflicts of interest to declare.

Funding Sources

J.C.H. is supported by VA Merit Award, NIH 1R01DK078897, NIH 1R01DK088541, NIH P01-DK-56492; K.L. is supported by NIH R01 DK117913.

Author Contributions

Y.C. and J.C.H. wrote the manuscript; K.L. and Z.N. revised manuscript.