There is substantial evidence indicating the possible involvement of rotavirus in biliary atresia (BA) development, at least in a subset of patients, but concrete proof remains lacking. In a mouse model, it has been well demonstrated that rotavirus can infect the biliary epithelium to cause biliary inflammation and obstruction, representing the pathogenesis of BA in humans. By using recently developed organoids technology, we now have demonstrated that human biliary organoids are susceptible to rotavirus infection, and this provokes active virus-host interactions and causes severe cytopathogenesis. Thus, our model recapitulates some essential aspects of BA development. Furthermore, we have demonstrated that antiviral drugs and neutralizing antibodies are capable of counteracting the infection and BA-like morphological changes, suggesting their potential for mitigating BA in patients.
KEYWORDS: biliary atresia, rotavirus infection, human organoids
ABSTRACT
Biliary atresia (BA) is a neonatal liver disease characterized by progressive fibroinflammatory obliteration of both intrahepatic and extrahepatic bile ducts. The etiologies of BA remain largely unknown, but rotavirus infection has been implicated at least for a subset of patients, and this causal relation has been well demonstrated in mouse models. In this study, we aim to further consolidate this evidence in human biliary organoids. We obtained seven batches of human biliary organoids cultured from fetal liver, adult liver, and bile duct tissues. We found that these organoids are highly susceptible and support the full life cycle of rotavirus infection in three-dimensional culture. The robust infection triggers active virus-host interactions, including interferon-based host defense mechanisms and injury responses. We have observed direct cytopathogenesis in organoids upon rotavirus infection, which may partially recapitulate the development of BA. Importantly, we have demonstrated the efficacy of mycophenolic acid and interferon alpha but not ribavirin in inhibiting rotavirus in biliary organoids. Furthermore, neutralizing antibody targeting rotavirus VP7 protein effectively inhibits infection in organoids. Thus, we have substantiated the causal evidence of rotavirus inducing BA in humans and provided potential strategies to combat the disease.
OBSERVATION
Biliary atresia (BA) is characterized by progressive fibroinflammatory obliteration of the bile ducts, resulting in chronic cholestasis and biliary cirrhosis. It is one of the leading causes for liver transplantation in infants (1, 2). Exposure to rotavirus in mice has demonstrated the infection in biliary epithelium, resulting in BA-like biliary inflammation and obstruction (3). Nevertheless, whether rotavirus is a causal agent for BA in patients remains controversial, also because of a paucity of preclinical models. Organoid technology provides an excellent way forward here. These three-dimensional (3D) cultured organoids are superior in recapitulating the architecture, composition, diversity, organization, and functionality of cell types of the tissue/organ of origin. Human organoids have been increasingly explored to advance research in disease modeling (4, 5). Although it is feasible to culture hepatocyte-like organoids from liver tissue, it remains technically challenging, with requirements for stringent experimental protocols (6). In contrast, organoids resembling the cholangiocyte phenotype are relatively easy to culture from the hepatic and extrahepatic bile duct compartments (7–9). In this study, we explored the feasibility of employing human biliary organoids cultured from fetal liver, adult liver, and bile duct for recapitulating BA development.
The canonical compartment for rotavirus infection is the small intestinal enterocyte. We previously showed that human intestinal organoids (HIOs) sustain rotavirus infection (4), and we confirmed these results (see Fig. S1A to C in the supplemental material). BA is a disorder that typically first manifests itself during mid-gestation, and murine experimentation has demonstrated rotavirus-induced BA development. Hence, we first tested if biliary fetal liver organoids (FLOs) support rotavirus infection. Inoculation of FLOs with rotavirus resulted in an increase of cellular viral RNA by a factor of 103 to 105 at 24 h and 104- to 106-fold at 48 h postinoculation (Fig. 1A) with a concomitant increase in levels of rotavirus VP4 protein (Fig. 1B). Thus, the human fetal biliary epithelium is highly permissive for rotavirus infection comparable to the level in intestinal epithelium (Fig. S1A). In apparent agreement, supernatant harvested from infected FLOs effectively infected and replicated in a Caco2 intestinal epithelial model as shown by reverse transcription-quantitative PCR (qRT-PCR) of viral RNA (Fig. 1C). Next, we performed a 50% tissue culture infective dose (TCID50) assay to compare the levels of infectious viral particles between the baseline of inoculation and five batches of organoids at 48 h postinoculation. We harvested rotavirus from the organoids through repeated freezing and thawing and demonstrated 102- to 103-fold increase of infectious virus titers (Fig. 1D). This was further confirmed by cytopathic effects in Caco2 cells at 48 h postinoculation with rotavirus harvested from these five batches of organoids and the control (see Fig. S2 in the supplemental material). Collectively, these results convincingly showed effective replication and production of infectious viral particles by infected fetal biliary organoids. Similar results were obtained in biliary organoids derived from adult human liver and bile duct (Fig. 1A to C). Thus, the human biliary epithelium is highly susceptible to rotavirus infection and supports its full life cycle.
To better understand the consequences of rotavirus infection in biliary epithelium, we performed a genome-wide transcriptomic analysis of FLOs upon infection. Volcano plots of the results showed significant downregulation of 103 and upregulation of 512 genes in response to rotavirus compared to that in uninfected organoids (Fig. 1E). Most of the highly upregulated genes, including IFIT2, IFITM3, OASL, DDX58, MX2, IFI35, HERC5, and BST2, are interferon-stimulated genes (ISGs). Other genes, such as CXCL11 and NLRC5 are related to the inflammatory response. Gene ontology (GO) enrichment analysis of these differentially expressed genes confirmed the essential involvement of the “immune system process” (Fig. 1F). Interestingly, “response to stress,” “cell death,” and “extracellular space” were also identified as the top regulated processes, with obvious relations to the development and pathogenesis of BA (Fig. 1E).
This is in line with the observations that naive organoids grow and become hyaline in a spheroidal shape, whereas rotavirus-infected organoids are opaque, shriveled, and disorganized (Fig. 2A, top). Propidium iodide (PI) staining marked the wide spread of dead cells in infected organoids (Fig. 2A, middle). Confocal analysis after immunostaining of viral VP6 protein further visualized the disruption of infected organoid cells (Fig. 2A, bottom). Quantitative analysis demonstrated significant increases of the percentages of deteriorated biliary organoids at 12, 24, and 48 h postinfection of rotavirus (Fig. 2B). Thus, rotavirus infection causes severe cytopathogenesis in human biliary organoids.
Next, we evaluated a monoclonal neutralizing antibody targeting rotavirus VP7 protein (10) by using three representative batches of biliary organoids. It effectively inhibited rotavirus infection in a dose-dependent manner (Fig. 2C). Finally, the effects of the known broad-spectrum antiviral drugs were tested in all batches of organoids. Similarly to that in HIOs (see Fig. S1D in the supplemental material), mycophenolic acid (MPA) and interferon alpha (IFN-α) potently inhibited rotavirus in all batches of biliary organoids (Fig. 2D). Surprisingly, ribavirin was effective in intestinal (Fig. S1D) but not in biliary (Fig. 2D) organoids. Therefore, antiviral drugs and neutralizing antibodies are potential therapeutics to combat rotavirus infection in the human biliary epithelium compartment.
Discussion and conclusions.
Although the etiologies and pathogenesis of BA remain largely unknown, multiple pathogenic mechanisms are likely involved, including genetic mutations (11), exposure to environmental toxins (12), dysregulation of the immune system, and, most intriguingly, viral factors in a particular rotavirus (3, 13–18). Previous studies have attempted to detect rotavirus in liver or biliary tissues and the antibody in sera from BA patients, but results are inconclusive (19). Since the wide implementation of vaccines that have substantially counteracted rotavirus-mediated diarrheal disease, a more direct investigation on the causality of rotavirus infection for BA has become possible. A survey of the national registry system in Taiwan found a decreased incidence of BA from 2004 to 2009, mirroring the increased uptake of rotavirus vaccination (20). A nationwide population-based study in Korea has shown that rotavirus infection in neonates is a risk factor for BA, although vaccination did not impact disease incidence (21).
Unfortunately, detection of rotavirus in tissue is often not feasible, as advanced disease is usually diagnosed in children 4 to 6 weeks old and the virus likely has been cleared by that time. Here we show, using organoid technology, that the human biliary epithelium supports the full life cycle of rotavirus infection and results in cellular and morphological changes consistent with BA development, even in the absence of immune cell components in our model. Furthermore, we identify therapeutic strategies potentially useful for combating rotavirus infection in the biliary epithelium.
Interestingly, a study in mice has demonstrated that maternal vaccination can prevent rotavirus-induced BA in newborn pups (22). This is in line with our findings that neutralizing antibodies inhibit rotavirus infection in organoids. Thus, we have substantiated the causal evidence of rotavirus inducing BA in humans and provided potential strategies to combat the disease.
Materials and methods.
Human fetal liver organoids (FLOs; n = 3 batches) were initiated from 17-week-old human fetal livers collected at abortion, from adult liver (LiOs; n = 2 batches), and from adult bile duct (BDOs; n = 2 batches). Human intestinal organoids (HIOs; n = 1 batch) were cultured to serve as a standard model for rotavirus infection. Detailed methods are described in Text S1.
Data availability.
The complete dataset was deposited at Data Archiving and Networked Services (DANS) (https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:179259; https://doi.org/10.17026/dans-27b-j8k9) (see Text S1 for details).
ACKNOWLEDGMENTS
We declare no competing interests.
This research was supported by a VIDI grant (no. 91719300) from the Netherlands Organization for Scientific Research (NWO) and the KWF Young Investigator grant (no. 10140) from the Dutch Cancer Society to Q. Pan, the China Scholarship Council for funding Ph.D. fellowships (201606760056 to S.C., 201808370170 to P.L., and 201903250082 to Y.W.), and a grant from the Dutch Society for the Replacement of Animal Testing and ZONMW (11 4021 502) to M.P.P.
Footnotes
Citation Chen S, Li P, Wang Y, Yin Y, de Ruiter PE, Verstegen MMA, Peppelenbosch MP, van der Laan LJW, Pan Q. 2020. Rotavirus infection and cytopathogenesis in human biliary organoids potentially recapitulate biliary atresia development. mBio 11:e01968-20. https://doi.org/10.1128/mBio.01968-20.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
The complete dataset was deposited at Data Archiving and Networked Services (DANS) (https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:179259; https://doi.org/10.17026/dans-27b-j8k9) (see Text S1 for details).