Figure 2: AOCS molecular subtypes can be distinguished by stromal and tumor gene expression.
Analysis of gene expression profiles of microdissected epithelial tumor cells and stroma from four C1 tumors identified a 688-gene ovarian tumor-stroma gene signature (FDR-adjusted p<0.01). The signature contained 461 genes that were over-expressed in microdissected stroma and 227 genes with increased expression in epithelial tumor cells, and these distinguished ovarian tumor and stroma in an independent MGH dataset (Figure S2). The heatmap shows the tumor and stromal gene expression profiles in the AOCS’s HGSOC tumors and microdissected C1 stroma and tumor samples. C1 microdissected stroma samples clustered with C1 subtype tumors, while the C1 microdissected tumor samples clustered with C4 subtype tumors. All of these analyses were done using unsupervised hierarchical cluster analysis. Colors of the heatmap were scaled (z-score values across rows).
