Figure 4.

Fgl2 deficiency ameliorated liver steatosis in HFD-induced NASH by regulating lipid metabolism. In MCD-fed (A) or HFD-fed (B) WT and fgl2-/-mice, oil red O staining was performed to detect liver steatosis. The levels of cholesterol and triglycerides in the liver were examined (C, D). Hepatic mRNA levels of genes involved in lipogenesis (Fasn, SREBP-2) (E, F) or lipolysis (PPARα, CPT1A) (G, H) were tested by real-time PCR. For bar graphs, n=8 in each group. The data represent the mean ± SD from at least three independent experiments. Statistical differences were determined by two-way ANOVA. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.