Figure 5.

Drug Treatments Induced Neuronal Activity Changes in the 3D Cortical Culture

(A) Representative traces of Ca⁺⁺ transients from individual ROIs in 3-weeks-old 3D cortical culture samples at baseline condition and post-treatments of GABA_A receptor antagonist bicuculline (BIC, 10 μM), GABA_A and GABAρ receptors antagonist picrotoxin (PTX, 50 μM), NMDA receptor antagonist AP5 (50 μM), or AMPA receptor antagonist NBQX (5 μM). Fluorescence changes were normalized to the basal fluorescence intensity (dF/F₀).

(B) Percentage of ROIs in each culture sample containing at least one Ca⁺⁺ event at baseline and post-treatments.

(C) Average Ca⁺⁺ events of all ROIs per sample.

(D) Global synchronization index of Ca⁺⁺ transients across all ROIs in a sample. For (B–D), gray data points and lines show baseline and post-treatment measurements of the same 3D tissue culture samples, and color lines show mean ± SEM. n = 9–12 per group from three to four independent experiments. Paired t tests were used for statistical significance comparing baseline and post-treatment of the same sample. ∗p < 0.05, ∗∗p < 0.001. See also Figure S3, Table S1, and Videos S2, S3, S4, and S5.