. 2020 Aug 19;6(8):e04602. doi: 10.1016/j.heliyon.2020.e04602

Table 5.

Effect of EaF10 on the enzymatic and non-enzymatic antioxidant in the liver.

Name of the enzymatic/non-enzymatic antioxidant	Level of the enzymatic (activity)/non-enzymatic (content) antioxidant
Name of the enzymatic/non-enzymatic antioxidant	Control	CCl₄	CCl₄ + Sil (100mg/Kg)	CCl₄ + EaF10 (25mg/Kg)	CCl₄ + EaF10 (100mg/Kg)
SOD (Unit/min/mg protein)	72.36 ± 2.47	39.44 ± 1.87^a	63.88 ± 2.31^b	57.12 ± 2.58^b	66.29 ± 3.16^b
CAT (Unit/min/mg protein)	19.28 ± 1.22	6.39 ± 1.53^a	16.83 ± 2.44^b	11.52 ± 2.84^b	17.13 ± 1.59^b
GST (nmol/min/mg protein)	0.77 ± 0.03	0.21 ± 0.01^a	0.69 ± 0.02^b	0.47 ± 0.01^b	0.70 ± 0.02^b
GSH (nmol/mg protein)	25.64 ± 1.41	10.76 ± 2.17 ^a	21.03 ± 1.54 ^b	15.69 ± 2.57 ^b	22.74 ± 2.33^b

Mice in different groups were treated as indicated in the material and methods section. Effect of EaF10 on the level of the enzymatic (activity) and non-enzymatic (content) antioxidants in the liver was determined. Values are expressed as means ± SD, n = 6; ^a values significantly different compared to normal control group (P˂0.05); ^b values significantly different compared to CCl₄-intoxicated group (P˂0.05) using Bonferroni's test. Control: normal control group; CCl₄: CCl₄-intoxicated group alone; CCl₄+Sil (100mg/Kg): CCl₄-intoxicated + silymarin (100mg/Kg)-treated group; CCl₄ + EaF10 (25mg/Kg): CCl₄-intoxicated + EaF10 (25mg/Kg)-treated group; CCl₄ + EaF10 (100mg/Kg): CCl₄-intoxicated + EaF10 (100mg/Kg)-treated group. SOD: Superoxide dismutase; CAT: Catalase; GST: Glutathione-s-transferase; GSH: Reduced glutathione.