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Oxford University Press - PMC COVID-19 Collection logoLink to Oxford University Press - PMC COVID-19 Collection
. 2020 Jul 9:ciaa941. doi: 10.1093/cid/ciaa941

A Confirmed Case of SARS-CoV-2 Pneumonia with Routine RT-PCR Negative and Virus Variation in Guangzhou, China

Zhengtu Li 1,2, Yinhu Li 2,2, Lingdan Chen 1,2, Shaoqiang Li 1, Le Yu 2, Airu Zhu 1, Feng Yang 1, Qian Jiang 1, Liyan Chen 1, Jincun Zhao 1, Wenju Lu 1, Nanshan Zhong 1, Feng Ye 1,
PMCID: PMC7454436  PMID: 32642757

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine RT-PCR detection of SARS-CoV-2. However, we report a confirmed case of SARS-CoV-2 pneumonia with routine RT-PCR negative. This case has been finally diagnosed by Nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variation of SARS-CoV-2 were found. This case has highlighted that false negative results could be present in the routine RT-PCR diagnosis, especially with virus variation. At the moment, Nanopore pathogen sequencing and antibody detection have been found effective in clinical diagnosis.

Keywords: SARS-CoV-2, Pneumonia, Routine RT-PCR, Virus variation, Nanopore sequencing


Articles from Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America are provided here courtesy of Oxford University Press

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