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. 2020 Jun 25;69(9):1948–1960. doi: 10.2337/db20-0248

Figure 6.

Figure 6

Transplantation of PVPON/TA-encapsulated islets abrogate T-cell infiltration and effector responses. C57BL/6 islet allografts were excised from STZ-treated NOD mice at day 5 posttransplantation for quantitative RT-PCR analysis of T-cell markers Cd8a (A), Grzmb (B), and Prf1 (C) normalized to Gapdh. Flow cytometry at day 7 posttransplantation of islet-infiltrating CD8 T-cell numbers (D), frequency (E) and cell number (F) of perforin+ CD8 T cells, frequency (G) and cell number (H) of CTLA-4+ CD8 T cells, frequency (I) and cell number (J) of CTLA-4+ CD25+ CD8 T cells, frequency (K) and cell number (L) of CTLA-4+ CD44+ CD8 T cells, and frequency (M) and cell number (N) of CTLA-4+ PD-1+ CD8 T cells (n = 5–10). Analyzed by two-way ANOVA with multiple comparisons. Kidney islet allografts were excised 40 days posttransplant, sectioned, and then stained with immunofluorescence to detect CD4 or CD8 T cells (red), insulin (green), or DAPI in nonencapsulated and PVPON/TA-encapsulated islets (O). Data represent three independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0005.