Figure 4.

Deletion of PDK1 enhances anti-tumor immunity and enhances LPS-induced acute inflammation by enhancing NK cell activation.

(a) Representative flow cytometry plots of NK cells (CD45⁺CD3⁻NKp46⁺) in breast tumor tissues of the specified genotypes at 20 weeks of age (upper). Analysis of IFN-γ production by NK cells after restimulation with PMA/ionomycin (below). Each symbol represents an individual mouse. (b) Similar to A. Representative flow cytometry plots of CD8⁺ T cell (CD45⁺CD3⁺CD8⁺), and analysis of IFN-γ production by CD8⁺ T cells. Each symbol represents an individual mouse. (c-d) Representative plots showing IFN-γ and TNF-α expression in CD45⁺CD3⁻NKp46⁺ NK cells (C) and CD45⁺CD3⁺CD8⁺ T cells (d) in B16-GMCSF tumor from the specified genotypes after re-stimulation with PMA/ionomycin. Each symbol represents an individual mouse. Data shown represent at least three independent experiments. *P < .05, **P < .01, ***P < .001. The data were analyzed using the Unpaired Student’s t-tests (two-tailed) (a-d).