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. 2020 Aug 11;8(8):283. doi: 10.3390/biomedicines8080283

Figure 5.

Figure 5

Effects of hypoxia preconditioned blood-derived secretomes obtained with or without oral anticoagulation (OA) on microvessel formations in human umbilical vein endothelial cell (HUVEC) cultures in vitro. (A) Panel showing representative images of the tube formation assay (12 h) carried out in the presence of blood-derived secretomes (HPP, HPS, normal plasma, and normal serum) obtained from subjects that did not receive oral anticoagulation (OA) or were treated with acetylsalicylic acid (ASA), ASA + clopidogrel, or nonvitamin K antagonist oral anticoagulants (NOACs) (Bars = 100 μm). (B) Plots showing the mean number of tubes and (C) nodes formed in HUVEC cultures that were incubated for 12 h with blood-derived secretomes. Capital letter pairs over plots indicate statistical comparisons of the corresponding data points. For all pair comparisons, p < 0.05, unless otherwise indicated. Error bars represent s.d.; number of subjects tested: no OA, n = 5; ASA, n = 8; ASA + clopidogrel, n = 10; NOACs, n = 7; normal plasma (no OA), n = 5; normal serum (no OA), n = 5; phosphate-buffered saline (PBS), n = 3; and VEGF, n = 3. Three samples were tested per subject per condition.