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. 2020 Aug 8;9(8):1859. doi: 10.3390/cells9081859

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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BC200 knockdown inhibited cell migration, invasion, colony formation, and sphere formation in GB cells in vitro. (A) The level of BC200 transfection with shBC200 was analyzed using RT-qPCR. (B) Wound-healing assay or migration assay showed that shBC200 resulted in delayed healing of the scratch wound. (C) Transwell invasion assay was used and results were expressed as the number of invaded cells per field. (D) The levels of N-cadherin, vimentin, and slug following shBC200 in GB cells were determined through western blot. (E) Colony formation assays showed that BC200 knockdown inhibited GB cell survival. (F) CCK-8 assay showed that BC200 knockdown had no effect on GB cell proliferation. (G) Neurosphere formation assays showed that BC200 knockdown inhibited GB cell stemness. (H) The levels of SOX2, Oct4, and KLF4 following shBC200 transfection in GB cells were determined through western blot analysis. * p < 0.05, ** p < 0.01, and *** p < 0.001.