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. 2020 Aug 8;9(8):1859. doi: 10.3390/cells9081859

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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BC200 RNA overexpression promotes cell migration, invasion, colony formation, and sphere formation on GB cells in vitro. (A) The level of BC200 transfection with OEBC200, an expression vector, was analyzed using RT-qPCR. (B) Migration assay showed that OEBC200 resulted in fast scratch wound healing. (C) Transwell invasion assay was performed, and the results were expressed as the number of invaded cells per field. (D) Following OEBC200 treatment, the levels of N-cadherin, vimentin, and slug in GB cells were determined through western blot. (E) Colony formation assays showed that BC200 RNA overexpression enhances GB cell survival. (F) CCK-8 assay showed that OEBC200 had no effect on GB cell proliferation. (G) Neurosphere formation assays showed that a high expression of BC200 promotes GB cell stemness. (H) Following OEBC200 treatment, the levels of SOX2, Oct4, and KLF4 in GB cells were determined through western blot. * p < 0.05, ** p < 0.01, and *** p < 0.001.