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. 2020 Jul 22;12(8):691. doi: 10.3390/pharmaceutics12080691

Figure 2.

Figure 2

Minimum biofilm eradicating concentration (MBEC) assay on PA14 wt biofilm grown in PPGAS medium for 24 h: biofilms were treated for 24 h with (A) free tobramycin (Tob); (B) Tob combined with itaconic acid (IA, molar ratio [Tob]:[IA] of 1:5); (C) free ciprofloxacin (Cipro); (D) Cipro combined with IA (molar [Cipro]:[IA] of 1:5). Cfu/mL values are depicted logarithmically for n = 3 experiments, each with technical triplicates. Untreated PA14 wt biofilms served as controls. The dotted lines indicate the detection limit. * is for p < 0.001 vs. controls; # and & are for p < 0.001 vs. samples treated with Tob or Cipro at the concentration of 6.25 and 12.5 µg/mL, respectively. • represents mean value.