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. 2020 Aug 6;25(16):3578. doi: 10.3390/molecules25163578

Figure 4.

Figure 4

Dihydroethidium (DHE) histofluorescence (A) and 4-HNE immunohistochemistry (B) of the vehicle/sham (A(a),C(a)), CGA/sham (A(d),C(d)), vehicle/TFI (A(b,c),C(c,d)) and CGA/TFI (A(e,f),C(e,f)) groups at 1 and 5 days after TFI. DHE fluorescence and 4-HNE immunoreactivity in the vehicle/sham group are shown in CA1 pyramidal cells located in the SP. DHE fluorescence and 4-HNE immunoreactivity in CA1 pyramidal cells of the vehicle/TFI group are markedly enhanced after TFI, but CGA treatment apparently reduces their levels after TFI. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 40 μm. C and D: relative fluorescence of DHE (C) and relative immunoreactivity (RI) of 4-HNE (D) in the CA1 SP. The bars indicate the means ± SEM (n = 7 at each point in time, * p < 0.05 vs. the vehicle/sham group, † p < 0.05 vs. the corresponding time point vehicle group, # p < 0.05 vs. the pretime point vehicle/TFI group).