Skip to main content
. 2020 Aug 14;25(16):3707. doi: 10.3390/molecules25163707

Figure 4.

Figure 4

PGN derivatives incorporated into the lipid bilayer further augment immune responses as observed on the mRNA and protein level. (a) 1:5 PGN derivatives/PC mixtures were sonicated and DLS was measured. PGN derivatives (200 μM PGN derivatives/1mM PC mixtures) were preincubated for 1 h and then stimulated with LPS (1 ng/mL) for 4 h for qPCR and for 16h for the ELISA assay. RNA was isolated and qPCRs was performed; (b) for Il6 and (c) for Rantes. ELISA assay was performed for Il-6 (c) with 5 ng/mL LPS and for RANTES (d). Statistics: Student’s t-Test * p < 0.05, ** p < 0.01, *** p < 0.001. Statistics: Student’s t-Test * p < 0.05, ** p < 0.01, *** p < 0.001, n.s.—not significant. As control unstimulated cells were used. Representative experiments from 3 independent experiments are shown.