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Effect of inhibitors on crosslinking-induced co-localization of MHC-II and clathrin. BMDCs pre-incubated with (A) control (0.1% DMSO), (B) BAPTA-AM (50 μM), (C) R406 (3 μM), or (D) U73122 (10 μM) for 30 min at 37 °C. The cell surface MHC-II was crosslinked with anti-MHC-II antibody. The co-localization of MHC-II and CHC was analyzed as described in Figure 7. Values are presented as the mean ± SEM (n = 10), * p < 0.05, ** p < 0.01.
