Figure 3.

Effects of a DHA-rich diet on inflammatory mediator release and neutrophil extracellular trap (NET) formation following acute exposure to HDE. (A–H) BALF was collected from mice and screened for various cytokines and chemokines via ELISAs. (I) NET formation was assessed by scoring the BALF cytospins across all three experimental trials. The top line represents the main effect of HDE vs. saline with significance represented by p-values. Tukey post hoc comparisons are provided for DHA- vs. control-diet-treated conditions and are annotated by the # symbol (# p < 0.05). Significant differences between HDE- and saline-treated conditions within diet groups are represented by the * symbol above the HDE bars (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). Any significant interactions between diet and exposure are depicted by the + symbol (+ p < 0.05).