Table 4.
Analysis of Composition of Microbiomes (ANCOM) results showing percentile abundances of taxa at genus level and W-statistics for high-trimethylamine-N-oxide (TMAO) producers (n = 17; those with ≥40% urinary TMAO increase from 0 min study-baseline in response to choline bitartrate) versus low-TMAO producers (n = 17; those with <40% urinary TMAO increase from 0 min study-baseline in response to choline bitartrate).
| High-TMAO Producers | Low-TMAO Producers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Taxa | 0% | 25% | 50% | 75% | 100% | 0% | 25% | 50% | 75% | 100% | W | Reject Null Hypothesis |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Clostridium | 1 | 1 | 1 | 78 | 158 | 1 | 1 | 1 | 1 | 1 | 11 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__Clostridium | 87 | 152 | 263 | 385 | 1138 | 1 | 37 | 96 | 194 | 340 | 8 | TRUE |
| k__Bacteria; p__Bacteroidetes; c__Bacteroidia; o__Bacteroidales; f__S24-7; g__ | 1 | 1 | 1 | 1 | 5 | 1 | 1 | 1 | 49 | 100 | 2 | TRUE |
| k__Bacteria; p__Firmicutes; c__Bacilli; o__Lactobacillales; f__Streptococcaceae; g__Lactococcus | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 68 | 2 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Christensenellaceae; g__ | 1 | 1 | 1 | 1 | 125 | 1 | 1 | 8 | 90 | 420 | 2 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Clostridiaceae; g__ | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 67 | 2 | TRUE |
| k__Bacteria; p__Bacteroidetes; c__Bacteroidia; o__Bacteroidales; f__; g__ | 1 | 1 | 1 | 1 | 9 | 1 | 1 | 1 | 1 | 1876 | 2 | TRUE |
| k__Bacteria; p__Cyanobacteria; c__4C0d-2; o__YS2; f__; g__ | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 126 | 2 | TRUE |
| k__Bacteria; p__Firmicutes; c__Erysipelotrichi; o__Erysipelotrichales; f__Erysipelotrichaceae; g__Catenibacterium | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 83 | 2 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Oscillospira | 1 | 80 | 143 | 354 | 1359 | 53 | 124 | 162 | 264 | 432 | 1 | TRUE |
| k__Bacteria; p__Firmicutes; c__Bacilli; o__Gemellales; f__Gemellaceae; g__Gemella | 1 | 1 | 1 | 1 | 5 | 1 | 1 | 1 | 1 | 10 | 1 | TRUE |
| k__Bacteria; p__Bacteroidetes; c__Bacteroidia; o__Bacteroidales; f__Rikenellaceae; g__Alistipes | 1 | 90 | 167 | 485 | 6008 | 90 | 150 | 154 | 233 | 478 | 1 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Butyricicoccus | 1 | 1 | 1 | 56 | 166 | 1 | 1 | 36 | 85 | 175 | 1 | TRUE |
| k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__ | 1 | 85 | 254 | 661 | 1205 | 1 | 196 | 340 | 653 | 2396 | 1 | TRUE |
Values are abundance of reads for each percentile within the high-TMAO producer versus low-TMAO producer groups. W-statistic indicates the strength of the ANCOM test (all at p < 0.05).