Figure 4.
ABCC11-inhibitory activities for each fraction of soybean extract from the first separation step with preparative medium-pressure liquid chromatography (MPLC). (a) A preparative MPLC chromatogram for separating the water extract of soybeans. The chromatogram was recorded at 265 nm. Red line indicates linear gradients of solvent B (0.2% formic acid in acetonitrile). (b) ABCC11-inhibitory activity profile of each fraction (100 ppm) obtained from the first separation process. The effects on ABCC11-mediated [1,2,6,7-3H(N)]-DHEA-S transport activity were investigated by the vesicle transport assay; 1% methanol was used for the vehicle control. Data are expressed as % of vehicle and the mean ± SD; n = 3. **, p < 0.01 vs. control (Dunnett’s test).