Table A3.
Recovery (n = 10) of vitamin D metabolites in plasma, breastmilk, and infant formula at inter-day.
| Samples | Analytes | ||
|---|---|---|---|
| Plasma (n = 10) | 25(OH)D2 | 25(OH)D3 | 3-Epi25(OH)D3 |
| Low | |||
| Added (nmol/L) | 26.47 | 46.36 | 27.96 |
| Measured (nmol/L) | 30.22 ± 2.66 | 42.93 ± 3.41 | 34.50 ± 2.18 |
| Recovery (%) | 114 | 93 | 128 |
| Medium | |||
| Added (nmol/L) | 74.94 | 96.28 | 51.92 |
| Measured (nmol/L) | 71.19 ± 4.19 | 102.63 ± 5.59 | 48.18 ± 3.59 |
| Recovery (%) | 95 | 107 | 93 |
| High | |||
| Added (nmol/L) | 121.17 | 146.20 | 101.84 |
| Measured (nmol/L) | 115.33 ± 8.95 | 162.75 ± 8.99 | 99.94 ± 5.59 |
| Recovery (%) | 95 | 111 | 98 |
| Breastmilk (n = 10) | 25(OH)D2 | 25(OH)D3 | 3-Epi25(OH)D3 |
| Low | |||
| Added (nmol/L) | <LOQ | 15.18 | 8.07 |
| Measured (nmol/L) | 12.04 ± 2.04 | 5.83 ± 1.73 | |
| Recovery (%) | 79 | 72 | |
| Medium | |||
| Added (nmol/L) | 48.74 | 65.10 | 57.99 |
| Measured (nmol/L) | 39.89 ± 2.75 | 65.01 ± 5.19 | 45.74 ± 2.64 |
| Recovery (%) | 82 | 100 | 79 |
| High | |||
| Added (nmol/L) | 97.20 | 111.50 | 107.91 |
| Measured (nmol/L) | 97.88 ± 4.63 | 114.80 ± 7.98 | 91.98 ± 8.98 |
| Recovery (%) | 101 | 103 | 85 |
| Infant formula (n = 10) | 25(OH)D2 | 25(OH)D3 | 3-Epi25(OH)D3 |
| Low | |||
| Added (nmol/L) | <LOQ | <LOQ | <LOQ |
| Measured (nmol/L) | |||
| Recovery (%) | |||
| Medium | |||
| Added (nmol/L) | 26.48 | 51.53 | 24.96 |
| Measured (nmol/L) | 21.37 ± 2.35 | 66.63 ± 2.71 | 32.22 ± 1.80 |
| Recovery (%) | 81 | 129 | 129 |
| High | |||
| Added (nmol/L) | 99.18 | 101.45 | 99.84 |
| Measured (nmol/L) | 93.17 ± 8.67 | 112.08 ± 4.15 | 107.10 ± 3.61 |
| Recovery (%) | 94 | 110 | 107 |
Recovery (%) = 100 × measured concentration/amount added.