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. 2020 Jul 24;12(8):2206. doi: 10.3390/nu12082206

Table 2.

Concentration of volatile fatty acids (VFA) in ascending colon contents and feces 1.

Diet Pooled SEM p-Value
Item Control Test
Ascending Colon
DM, % 23.77 23.95 0.677 0.849
SCFA absolute, μmol/g DM
Acetate 47.34 56.40 6.043 0.304
Propionate 23.72 23.05 2.831 0.869
Butyrate 9.82 10.35 1.442 0.798
Total SCFA 80.88 89.80 9.800 0.528
SCFA relative, % of total
Acetate 59.12 63.00 2.334 0.067
Propionate 28.92 25.75 1.660 0.018
Butyrate 11.95 11.27 0.747 0.479
BCFA absolute, μmol/g DM
Isovalerate 5.61 4.32 0.703 0.203
Valerate 2.48 2.47 0.298 0.976
Isobutyrate 3.76 2.80 0.444 0.136
Total BCFA 12.30 9.58 1.553 0.222
BCFA relative, % of total
Isovalerate 45.96 44.96 1.808 0.319
Valerate 23.12 26.18 2.547 0.046
Isobutyrate 30.90 28.86 0.846 0.037
Feces
DM, % 30.15 32.00 1.922 0.437
SCFA absolute, μmol/g DM
Acetate 43.09 30.33 4.953 0.080
Propionate 15.59 9.80 2.912 0.111
Butyrate 5.91 3.83 1.586 0.154
Total SCFA 73.39 43.95 11.30 0.051
SCFA relative, % of total
Acetate 68.82 70.57 3.516 0.606
Propionate 21.40 21.43 1.362 0.986
Butyrate 9.86 7.99 2.309 0.294
BCFA absolute, μmol/g DM
Isovalerate 5.07 3.67 0.825 0.248
Valerate 2.31 1.48 0.466 0.116
Isobutyrate 3.20 2.33 0.485 0.224
Total BCFA 10.61 7.49 1.671 0.195
BCFA relative, % of total
Isovalerate 48.00 48.81 0.927 0.545
Valerate 21.56 19.79 1.349 0.305
Isobutyrate 30.45 31.40 0.815 0.279

1 Pigs received diets containing 0 (Control) or 250 (Test) mg of bovine milk OPN per L of mixed milk replacer during a 30-d feeding study (n = 11 male pigs for control group and 10 male pigs for test group). Bolded p-values denote differences between treatment means (p < 0.05). Abbreviations: BCFA, branched-chain fatty acids; DM, dry matter; OPN, osteopontin; SCFA, short-chain fatty acids; SEM, standard error of the mean; VFA, volatile fatty acids.